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Article: Dichlorodiphenyltrichloroethane specifically depletes dopaminergic neurons in primary cell culture

TitleDichlorodiphenyltrichloroethane specifically depletes dopaminergic neurons in primary cell culture
Authors
KeywordsDichlorodiphenyltrichloroethane
Insecticide
Neuronal cell death
Neurotoxicity
Parkinson's disease
Pesticide
Issue Date2003
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/nba
Citation
Neuroembryology, 2003, v. 2 n. 3, p. 95-102 How to Cite?
AbstractToxicity of dichlorodiphenyltrichloroethane (DDT) to dopaminergic neurons in primary cell culture was investigated in the present study. Developing neurons from the substantia nigra of neonatal rats were cultured. After treatments with different concentrations of DDT (5-12.5 μM), specific cell death of tyrosine-hydroxylase-immunoreactive dopaminergic neurons was observed in the culture by flow cytometric analysis. More than 60% of dopaminergic neurons were depleted after treatments with 10 and 12.5 μM of DDT. In addition, significant reductions of intensity levels of tyrosine hydroxylase immunofluorescence were observed in dopaminergic neurons after DDT treatments even at low concentrations of DDT. The present findings indicate that dopaminergic neurons are more susceptible to DDT toxicity than other types of neurons in the primary cell culture. Moreover, it is shown that the synthesis of dopamine in dopaminergic neurons is also depressed. Previous studies have demonstrated that perinatal exposure of DDT causes neurons to be more susceptible to neurotoxic damages in later adult life. The present findings thus provide evidence that dopaminergic neurons that are undergoing growth and development are targets of DDT neurotoxic effects. Exposure to DDT from contaminated environments is therefore a potential risk of onset of Parkinson's disease. Copyright © 2003 S. Karger AG, Basel.
Persistent Identifierhttp://hdl.handle.net/10722/43481
ISSN
2006 SCImago Journal Rankings: 0.179
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, KWen_HK
dc.contributor.authorChan, YSen_HK
dc.contributor.authorYung, KKLen_HK
dc.date.accessioned2007-03-23T04:46:35Z-
dc.date.available2007-03-23T04:46:35Z-
dc.date.issued2003en_HK
dc.identifier.citationNeuroembryology, 2003, v. 2 n. 3, p. 95-102en_HK
dc.identifier.issn1424-8522en_HK
dc.identifier.urihttp://hdl.handle.net/10722/43481-
dc.description.abstractToxicity of dichlorodiphenyltrichloroethane (DDT) to dopaminergic neurons in primary cell culture was investigated in the present study. Developing neurons from the substantia nigra of neonatal rats were cultured. After treatments with different concentrations of DDT (5-12.5 μM), specific cell death of tyrosine-hydroxylase-immunoreactive dopaminergic neurons was observed in the culture by flow cytometric analysis. More than 60% of dopaminergic neurons were depleted after treatments with 10 and 12.5 μM of DDT. In addition, significant reductions of intensity levels of tyrosine hydroxylase immunofluorescence were observed in dopaminergic neurons after DDT treatments even at low concentrations of DDT. The present findings indicate that dopaminergic neurons are more susceptible to DDT toxicity than other types of neurons in the primary cell culture. Moreover, it is shown that the synthesis of dopamine in dopaminergic neurons is also depressed. Previous studies have demonstrated that perinatal exposure of DDT causes neurons to be more susceptible to neurotoxic damages in later adult life. The present findings thus provide evidence that dopaminergic neurons that are undergoing growth and development are targets of DDT neurotoxic effects. Exposure to DDT from contaminated environments is therefore a potential risk of onset of Parkinson's disease. Copyright © 2003 S. Karger AG, Basel.en_HK
dc.format.extent430062 bytes-
dc.format.extent27136 bytes-
dc.format.extent75310 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.format.mimetypeapplication/pdf-
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/nbaen_HK
dc.relation.ispartofNeuroembryologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectDichlorodiphenyltrichloroethaneen_HK
dc.subjectInsecticideen_HK
dc.subjectNeuronal cell deathen_HK
dc.subjectNeurotoxicityen_HK
dc.subjectParkinson's diseaseen_HK
dc.subjectPesticideen_HK
dc.titleDichlorodiphenyltrichloroethane specifically depletes dopaminergic neurons in primary cell cultureen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1424-8522&volume=2&issue=3&spage=95&epage=102&date=2003&atitle=Dichlorodiphenyltrichloroethane+Specifically+Depletes+Dopaminergic+Neurons+in+Primary+Cell+Cultureen_HK
dc.identifier.emailLeung, KW: kwleung1@hku.hken_HK
dc.identifier.emailChan, YS: yschan@hku.hken_HK
dc.identifier.authorityLeung, KW=rp01674en_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1159/000074188en_HK
dc.identifier.scopuseid_2-s2.0-0345305402en_HK
dc.identifier.hkuros92146-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0345305402&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume2en_HK
dc.identifier.issue3en_HK
dc.identifier.spage95en_HK
dc.identifier.epage102en_HK
dc.publisher.placeSwitzerlanden_HK
dc.identifier.scopusauthoridLeung, KW=13106059300en_HK
dc.identifier.scopusauthoridChan, YS=7403676627en_HK
dc.identifier.scopusauthoridYung, KKL=13605496000en_HK

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