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Article: Identification of slide coagulase positive, tube coagulase negative Staphylococcus aureus by 16S ribosomal RNA gene sequencing

TitleIdentification of slide coagulase positive, tube coagulase negative Staphylococcus aureus by 16S ribosomal RNA gene sequencing
Authors
Keywords16S
Ribosomal
RNA
Sequencing
Issue Date2001
PublisherB M J Publishing Group. The Journal's web site is located at http://mp.bmjjournals.com/
Citation
Journal Of Clinical Pathology - Molecular Pathology, 2001, v. 54 n. 4, p. 244-247 How to Cite?
AbstractAims - To ascertain the clinical importance of a strain of slide coagulase positive but tube coagulase negative staphylococcus species isolated from the blood culture of a 43 year old patient with refractory anaemia with excessive blasts in transformation who had neutropenic fever. Methods - The isolate was investigated phenotypically by standard biochemical methods using conventional biochemical tests and two commercially available systems, the Vitek (GPI) and API (Staph) systems. Genotypically, the 16S ribosomal RNA (rRNA) gene of the bacteria was amplified by the polymerase chain reaction (PCR) and sequenced. The sequence of the PCR product was compared with known 16S rRNA gene sequences in the GenBank by multiple sequence alignment. Results - Conventional biochemical tests did not reveal a pattern resembling a known staphylococcus species. The Vitek system (GPI) showed that it was 94% S simulans and 3% S haemolyticus, whereas the API system (Staph) showed that it was 86.8% S aureus and 5.1% S warneri. 16S rRNA gene sequencing showed that there was a 0 base difference between the isolate and S aureus, 28 base difference between the isolate and S lugdunensis, 39 base difference between the isolate and S schleiferi, 21 base difference between the isolate and S haemolyticus, 41 base difference between the isolate and S simulans, and 23 base difference between the isolate and S voarneri, indicating that the isolate was a strain of S aureus. Vancomycin was subsequently prescribed and blood cultures taken four days after the start of treatment were negative. Conclusions - 16S rRNA gene sequencing was useful in ascertaining the clinical importance of the strain of slide coagulase positive but tube coagulase negative staphylococcus species isolated from blood culture and allowing appropriate management.
Persistent Identifierhttp://hdl.handle.net/10722/43133
ISSN
2005 Impact Factor: 2.576
2005 SCImago Journal Rankings: 0.122
PubMed Central ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorLeung, ASPen_HK
dc.contributor.authorLeung, KWen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2007-03-23T04:39:37Z-
dc.date.available2007-03-23T04:39:37Z-
dc.date.issued2001en_HK
dc.identifier.citationJournal Of Clinical Pathology - Molecular Pathology, 2001, v. 54 n. 4, p. 244-247en_HK
dc.identifier.issn1366-8714en_HK
dc.identifier.urihttp://hdl.handle.net/10722/43133-
dc.description.abstractAims - To ascertain the clinical importance of a strain of slide coagulase positive but tube coagulase negative staphylococcus species isolated from the blood culture of a 43 year old patient with refractory anaemia with excessive blasts in transformation who had neutropenic fever. Methods - The isolate was investigated phenotypically by standard biochemical methods using conventional biochemical tests and two commercially available systems, the Vitek (GPI) and API (Staph) systems. Genotypically, the 16S ribosomal RNA (rRNA) gene of the bacteria was amplified by the polymerase chain reaction (PCR) and sequenced. The sequence of the PCR product was compared with known 16S rRNA gene sequences in the GenBank by multiple sequence alignment. Results - Conventional biochemical tests did not reveal a pattern resembling a known staphylococcus species. The Vitek system (GPI) showed that it was 94% S simulans and 3% S haemolyticus, whereas the API system (Staph) showed that it was 86.8% S aureus and 5.1% S warneri. 16S rRNA gene sequencing showed that there was a 0 base difference between the isolate and S aureus, 28 base difference between the isolate and S lugdunensis, 39 base difference between the isolate and S schleiferi, 21 base difference between the isolate and S haemolyticus, 41 base difference between the isolate and S simulans, and 23 base difference between the isolate and S voarneri, indicating that the isolate was a strain of S aureus. Vancomycin was subsequently prescribed and blood cultures taken four days after the start of treatment were negative. Conclusions - 16S rRNA gene sequencing was useful in ascertaining the clinical importance of the strain of slide coagulase positive but tube coagulase negative staphylococcus species isolated from blood culture and allowing appropriate management.en_HK
dc.format.extent749378 bytes-
dc.format.extent30720 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.languageengen_HK
dc.publisherB M J Publishing Group. The Journal's web site is located at http://mp.bmjjournals.com/en_HK
dc.relation.ispartofJournal of Clinical Pathology - Molecular Pathologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsMolecular Pathology. Copyright © B M J Publishing Group.en_HK
dc.subject16Sen_HK
dc.subjectRibosomalen_HK
dc.subjectRNAen_HK
dc.subjectSequencingen_HK
dc.subject.meshAnemia, refractory, with excess of blasts - microbiologyen_HK
dc.subject.meshAnti-bacterial agents - therapeutic useen_HK
dc.subject.meshRna, bacterial - geneticsen_HK
dc.subject.meshRna, ribosomal, 16s - geneticsen_HK
dc.subject.meshSequence analysis, rna - methodsen_HK
dc.titleIdentification of slide coagulase positive, tube coagulase negative Staphylococcus aureus by 16S ribosomal RNA gene sequencingen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1366-8714&volume=54&issue=4&spage=244&epage=247&date=2001&atitle=Identification+of+slide+coagulase+positive,+tube+coagulase+negative+Staphylococcus+aureus+by+16S+ribosomal+RNA+gene+sequencingen_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1136/mp.54.4.244en_HK
dc.identifier.pmid11477139-
dc.identifier.pmcidPMC1187075-
dc.identifier.scopuseid_2-s2.0-0034902989en_HK
dc.identifier.hkuros74456-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034902989&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume54en_HK
dc.identifier.issue4en_HK
dc.identifier.spage244en_HK
dc.identifier.epage247en_HK
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridLeung, ASP=7403012546en_HK
dc.identifier.scopusauthoridLeung, KW=7401860831en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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