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Article: The prevalence and characterization of verotoxin-producing Eschericha coli isolated from cattle and pigs in an abattoir in Hong Kong

TitleThe prevalence and characterization of verotoxin-producing Eschericha coli isolated from cattle and pigs in an abattoir in Hong Kong
Authors
Issue Date2001
PublisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=HYG
Citation
Epidemiology And Infection, 2001, v. 126 n. 2, p. 173-179 How to Cite?
AbstractThe aim of the study was to define the prevalence of verotoxin-producing Escherichia coli (VTEC) in cattle and pigs in a Hong Kong abattoir. Faecal and carcass samples collected from 986 cattle and 487 pigs from an abattoir were tested for verotoxin (VT) by PCR and cytotoxicity assays. VTEC was isolated from 41.5 and 1.8% of cattle faecal and carcass samples and from 2.1 and 0.2% of porcine faecal and carcass samples, respectively. Amongst 409 VTEC isolates from cattle, 9 were serotype O157:H7 and eaeA+. The most prevalent vt genotype among bovine VTEC was vt1+ vt2+ (73.8%) and in porcine VTEC was vt2e+ (30%). None of the porcine VTEC isolates and 9.3% of the bovine VTEC isolates was eaeA+. The non-O157 serogroup VTEC isolates carrying eaeA and EHEC-hlyA belonged to serogroups O172, O15, O84, O91, O110 and O121. The local dietary preference for pork or chicken (rather than beef), the low VTEC carriage in pigs, the rarity of additional virulence factors (eaeA) in VTEC isolated from cattle may explain the apparently low incidence of human diarrhoeal disease associated with VTEC in Hong Kong hitherto. However, the presence of non-O157 VTEC strains carrying the eaeA virulence marker in cattle highlights the fact that sole reliance on sorbitol-MacConkey agar for screening human VTEC isolates may underestimate the human disease burden. The changing dietary habits of the population in Hong Kong reinforce the need for continued vigilance.
Persistent Identifierhttp://hdl.handle.net/10722/43131
ISSN
2015 Impact Factor: 2.515
2015 SCImago Journal Rankings: 1.320
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, PHMen_HK
dc.contributor.authorYam, WCen_HK
dc.contributor.authorNg, WWSen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.date.accessioned2007-03-23T04:39:34Z-
dc.date.available2007-03-23T04:39:34Z-
dc.date.issued2001en_HK
dc.identifier.citationEpidemiology And Infection, 2001, v. 126 n. 2, p. 173-179en_HK
dc.identifier.issn0950-2688en_HK
dc.identifier.urihttp://hdl.handle.net/10722/43131-
dc.description.abstractThe aim of the study was to define the prevalence of verotoxin-producing Escherichia coli (VTEC) in cattle and pigs in a Hong Kong abattoir. Faecal and carcass samples collected from 986 cattle and 487 pigs from an abattoir were tested for verotoxin (VT) by PCR and cytotoxicity assays. VTEC was isolated from 41.5 and 1.8% of cattle faecal and carcass samples and from 2.1 and 0.2% of porcine faecal and carcass samples, respectively. Amongst 409 VTEC isolates from cattle, 9 were serotype O157:H7 and eaeA+. The most prevalent vt genotype among bovine VTEC was vt1+ vt2+ (73.8%) and in porcine VTEC was vt2e+ (30%). None of the porcine VTEC isolates and 9.3% of the bovine VTEC isolates was eaeA+. The non-O157 serogroup VTEC isolates carrying eaeA and EHEC-hlyA belonged to serogroups O172, O15, O84, O91, O110 and O121. The local dietary preference for pork or chicken (rather than beef), the low VTEC carriage in pigs, the rarity of additional virulence factors (eaeA) in VTEC isolated from cattle may explain the apparently low incidence of human diarrhoeal disease associated with VTEC in Hong Kong hitherto. However, the presence of non-O157 VTEC strains carrying the eaeA virulence marker in cattle highlights the fact that sole reliance on sorbitol-MacConkey agar for screening human VTEC isolates may underestimate the human disease burden. The changing dietary habits of the population in Hong Kong reinforce the need for continued vigilance.en_HK
dc.format.extent196192 bytes-
dc.format.extent3548 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=HYGen_HK
dc.relation.ispartofEpidemiology and Infectionen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsEpidemiology and Infection. Copyright © Cambridge University Press.en_HK
dc.subject.meshAbattoirsen_HK
dc.subject.meshCattle - microbiologyen_HK
dc.subject.meshEscherichia coli o157 - isolation & purification - metabolismen_HK
dc.subject.meshShiga toxins - genetics - isolation & purificationen_HK
dc.subject.meshSwine - microbiologyen_HK
dc.titleThe prevalence and characterization of verotoxin-producing Eschericha coli isolated from cattle and pigs in an abattoir in Hong Kongen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0950-2688&volume=126&issue=2&spage=173&epage=179&date=2001&atitle=The+prevalence+and+characterization+of+verotoxin-producing+Escherichia+coli+isolated+from+cattle+and+pigs+in+an+abattoir+in+Hong+Kongen_HK
dc.identifier.emailYam, WC: wcyam@hkucc.hku.hken_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.authorityYam, WC=rp00313en_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1017/S0950268801005210en_HK
dc.identifier.pmid11349966en_HK
dc.identifier.pmcidPMC2869680-
dc.identifier.scopuseid_2-s2.0-0035023078en_HK
dc.identifier.hkuros60544-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035023078&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume126en_HK
dc.identifier.issue2en_HK
dc.identifier.spage173en_HK
dc.identifier.epage179en_HK
dc.identifier.isiWOS:000168695100002-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLeung, PHM=7401747852en_HK
dc.identifier.scopusauthoridYam, WC=7004281720en_HK
dc.identifier.scopusauthoridNg, WWS=36787042600en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK

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