File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Identification by 16s ribosomal RNA gene sequencing of an Enterobacteriaceae species from a bone marrow transplant recipient

TitleIdentification by 16s ribosomal RNA gene sequencing of an Enterobacteriaceae species from a bone marrow transplant recipient
Authors
Keywords16s ribosomal rna sequencing
Bone marrow transplantation
Issue Date2000
PublisherB M J Publishing Group. The Journal's web site is located at http://mp.bmjjournals.com/
Citation
Journal Of Clinical Pathology - Molecular Pathology, 2000, v. 53 n. 4, p. 211-215 How to Cite?
AbstractAims - To ascertain the clinical relevance of a strain of Enterobacteriaceae isolated from the stool of a bone marrow transplant recipient with diarrhoea. The isolate could not be identified to the genus level by conventional phenotypic methods and required 16S ribosomal RNA (rRNA) gene sequencing for full identification. Methods - The isolate was investigated phenotypically by standard biochemical methods using conventional biochemical tests and two commercially available systems, the Vitek (GNI+) and API (20E) systems. Genotypically, the 16S bacterial rRNA gene was amplified by the polymerase chain reaction (PCR) and sequenced. The sequence of the PCR product was compared with known 16S rRNA gene sequences in the GenBank database by multiple sequence alignment. Results - Conventional biochemical tests did not reveal a pattern resembling any known member of the Enterobacteriaceae family. The isolate was identified as Salmonella arizonae (73%) and Escherichia coli (76%) by the Vitek (GNI+) and API (20E) systems, respectively. 16S rRNA sequencing showed that there was only one base difference between the isolate and E coli K-12, but 48 and 47 base differences between the isolate and S typhimurium (NCTC 8391) and S typhi (St111), respectively, showing that it was an E coli strain. The patient did not require any specific treatment and the diarrhoea subsided spontaneously. Conclusions - 16S rRNA gene sequencing was useful in ascertaining the clinical relevance of the strain of Enterobacteriaceae isolated from the stool of the bone marrow transplant recipient with diarrhoea.
Persistent Identifierhttp://hdl.handle.net/10722/43129
ISSN
2005 Impact Factor: 2.576
2005 SCImago Journal Rankings: 0.122
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorLeung, PKLen_HK
dc.contributor.authorLeung, KWen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2007-03-23T04:39:32Z-
dc.date.available2007-03-23T04:39:32Z-
dc.date.issued2000en_HK
dc.identifier.citationJournal Of Clinical Pathology - Molecular Pathology, 2000, v. 53 n. 4, p. 211-215en_HK
dc.identifier.issn1366-8714en_HK
dc.identifier.urihttp://hdl.handle.net/10722/43129-
dc.description.abstractAims - To ascertain the clinical relevance of a strain of Enterobacteriaceae isolated from the stool of a bone marrow transplant recipient with diarrhoea. The isolate could not be identified to the genus level by conventional phenotypic methods and required 16S ribosomal RNA (rRNA) gene sequencing for full identification. Methods - The isolate was investigated phenotypically by standard biochemical methods using conventional biochemical tests and two commercially available systems, the Vitek (GNI+) and API (20E) systems. Genotypically, the 16S bacterial rRNA gene was amplified by the polymerase chain reaction (PCR) and sequenced. The sequence of the PCR product was compared with known 16S rRNA gene sequences in the GenBank database by multiple sequence alignment. Results - Conventional biochemical tests did not reveal a pattern resembling any known member of the Enterobacteriaceae family. The isolate was identified as Salmonella arizonae (73%) and Escherichia coli (76%) by the Vitek (GNI+) and API (20E) systems, respectively. 16S rRNA sequencing showed that there was only one base difference between the isolate and E coli K-12, but 48 and 47 base differences between the isolate and S typhimurium (NCTC 8391) and S typhi (St111), respectively, showing that it was an E coli strain. The patient did not require any specific treatment and the diarrhoea subsided spontaneously. Conclusions - 16S rRNA gene sequencing was useful in ascertaining the clinical relevance of the strain of Enterobacteriaceae isolated from the stool of the bone marrow transplant recipient with diarrhoea.en_HK
dc.format.extent1262641 bytes-
dc.format.extent30720 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.languageengen_HK
dc.publisherB M J Publishing Group. The Journal's web site is located at http://mp.bmjjournals.com/en_HK
dc.relation.ispartofJournal of Clinical Pathology - Molecular Pathologyen_HK
dc.rightsJournal of Clinical Pathology. Copyright © B M J Publishing Group.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject16s ribosomal rna sequencingen_HK
dc.subjectBone marrow transplantationen_HK
dc.subject.meshAdulten_HK
dc.subject.meshBacterial Typing Techniquesen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshBone Marrow Transplantationen_HK
dc.subject.meshDiarrhea - immunology - microbiologyen_HK
dc.subject.meshEnterobacteriaceae - classification - genetics - isolation & purificationen_HK
dc.subject.meshEnterobacteriaceae Infections - diagnosis - immunologyen_HK
dc.subject.meshFeces - microbiologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunocompromised Hosten_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.subject.meshRNA, Bacterial - geneticsen_HK
dc.subject.meshRNA, Ribosomal, 16S - geneticsen_HK
dc.titleIdentification by 16s ribosomal RNA gene sequencing of an Enterobacteriaceae species from a bone marrow transplant recipienten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9746&volume=53&issue=4&spage=211&epage=215&date=2000&atitle=Identification+by+16S+ribosomal+RNA+gene+sequencing+of+an+Enterobacteriaceae+species+from+a+bone+marrow+transplant+recipienten_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1136/mp.53.4.211en_HK
dc.identifier.pmid11040945-
dc.identifier.scopuseid_2-s2.0-0033863914en_HK
dc.identifier.hkuros54425-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033863914&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume53en_HK
dc.identifier.issue4en_HK
dc.identifier.spage211en_HK
dc.identifier.epage215en_HK
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridLeung, PKL=55085129100en_HK
dc.identifier.scopusauthoridLeung, KW=7401860831en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats