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Article: IS6110 based amplityping assay and RFLP fingerprinting of clinical isolates of Mycobacterium tuberculosis

TitleIS6110 based amplityping assay and RFLP fingerprinting of clinical isolates of Mycobacterium tuberculosis
Authors
KeywordsAmplityping
IS6110
Mycobacterium tuberculosis
PCR
RFLP analysis
Issue Date1995
PublisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/
Citation
Journal Of Clinical Pathology, 1995, v. 48 n. 10, p. 924-928 How to Cite?
AbstractAims. To evaluate the usefulness of two IS6110 based typing methods, an amplityping assay and restriction fragment length polymorphism (RFLP) analysis, for fingerprinting respiratory isolates of Mycobacterium tuberculosis. Methods. For amplityping, a pair of primers which amplify the intervening sequence between the repetitive insertion sequence IS6110 was used to generate a banding pattern which was confirmed by hybridisation. This assay was compared with conventional chromosomal DNA RFLP typing in the evaluation of 110 epidemiologically diverse isolates. Results. Polymerase chain reaction (PCR) amplityping generated a single pattern in Hong Kong Chinese strains, but two and four diverse patterns in Filipino and Vietnamese strains, respectively, and could be completed within four days. When compared with chromosomal DNA RFLP typing, which took three weeks to complete, four different RFLP patterns could be seen among the Chinese strains, while seven patterns were found in the Filipino and Vietnamese strains. No change in amplityping or RFLP patterns was found in 36 sequential isolates from the same patients after anti-tuberculosis treatment for up to 12 months, despite the emergence of resistance in three of these strains. No specific amplityping or RFLP pattern could be related to different patterns of drug susceptibility. Conclusion. PCR amplityping could be used initially as a rapid typing method to distinguish strains originating from different localities. This could be important for investigation of outbreaks of tuberculosis for example, in refugee camps.
Persistent Identifierhttp://hdl.handle.net/10722/43119
ISSN
2015 Impact Factor: 2.912
2015 SCImago Journal Rankings: 1.260
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYuen, KYen_HK
dc.contributor.authorChan, CMen_HK
dc.contributor.authorChan, KSen_HK
dc.contributor.authorYam, WCen_HK
dc.contributor.authorHo, PLen_HK
dc.contributor.authorChau, PYen_HK
dc.date.accessioned2007-03-23T04:39:19Z-
dc.date.available2007-03-23T04:39:19Z-
dc.date.issued1995en_HK
dc.identifier.citationJournal Of Clinical Pathology, 1995, v. 48 n. 10, p. 924-928en_HK
dc.identifier.issn0021-9746en_HK
dc.identifier.urihttp://hdl.handle.net/10722/43119-
dc.description.abstractAims. To evaluate the usefulness of two IS6110 based typing methods, an amplityping assay and restriction fragment length polymorphism (RFLP) analysis, for fingerprinting respiratory isolates of Mycobacterium tuberculosis. Methods. For amplityping, a pair of primers which amplify the intervening sequence between the repetitive insertion sequence IS6110 was used to generate a banding pattern which was confirmed by hybridisation. This assay was compared with conventional chromosomal DNA RFLP typing in the evaluation of 110 epidemiologically diverse isolates. Results. Polymerase chain reaction (PCR) amplityping generated a single pattern in Hong Kong Chinese strains, but two and four diverse patterns in Filipino and Vietnamese strains, respectively, and could be completed within four days. When compared with chromosomal DNA RFLP typing, which took three weeks to complete, four different RFLP patterns could be seen among the Chinese strains, while seven patterns were found in the Filipino and Vietnamese strains. No change in amplityping or RFLP patterns was found in 36 sequential isolates from the same patients after anti-tuberculosis treatment for up to 12 months, despite the emergence of resistance in three of these strains. No specific amplityping or RFLP pattern could be related to different patterns of drug susceptibility. Conclusion. PCR amplityping could be used initially as a rapid typing method to distinguish strains originating from different localities. This could be important for investigation of outbreaks of tuberculosis for example, in refugee camps.en_HK
dc.format.extent1230943 bytes-
dc.format.extent30720 bytes-
dc.format.extent3548 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/en_HK
dc.relation.ispartofJournal of Clinical Pathologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Clinical Pathology. Copyright © B M J Publishing Group.en_HK
dc.subjectAmplitypingen_HK
dc.subjectIS6110en_HK
dc.subjectMycobacterium tuberculosisen_HK
dc.subjectPCRen_HK
dc.subjectRFLP analysisen_HK
dc.subject.meshBacterial typing techniquesen_HK
dc.subject.meshMolecular sequence dataen_HK
dc.subject.meshDna, bacterial - analysisen_HK
dc.subject.meshMycobacterium tuberculosisen_HK
dc.subject.meshPolymorphism, restriction fragment lengthen_HK
dc.titleIS6110 based amplityping assay and RFLP fingerprinting of clinical isolates of Mycobacterium tuberculosisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9746&volume=48&issue=10&spage=924&epage=928&date=1995&atitle=IS6110+based+amplityping+assay+and+RFLP+fingerprinting+of+clinical+isolates+of+Mycobacterium+tuberculosisen_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.emailYam, WC:wcyam@hkucc.hku.hken_HK
dc.identifier.emailHo, PL:plho@hkucc.hku.hken_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.identifier.authorityYam, WC=rp00313en_HK
dc.identifier.authorityHo, PL=rp00406en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid8537491-
dc.identifier.pmcidPMC502948-
dc.identifier.scopuseid_2-s2.0-0028839739en_HK
dc.identifier.hkuros14577-
dc.identifier.volume48en_HK
dc.identifier.issue10en_HK
dc.identifier.spage924en_HK
dc.identifier.epage928en_HK
dc.identifier.isiWOS:A1995RZ57900010-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.scopusauthoridChan, CM=7404814453en_HK
dc.identifier.scopusauthoridChan, KS=36448818400en_HK
dc.identifier.scopusauthoridYam, WC=7004281720en_HK
dc.identifier.scopusauthoridHo, PL=7402211363en_HK
dc.identifier.scopusauthoridChau, PY=29267442500en_HK

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