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Article: Pseudobacteraemia in a patient with neutropenic fever caused by a novel paenibacillus species: Paenibacillus hongkongensis sp. nov.

TitlePseudobacteraemia in a patient with neutropenic fever caused by a novel paenibacillus species: Paenibacillus hongkongensis sp. nov.
Authors
Issue Date2003
PublisherB M J Publishing Group. The Journal's web site is located at http://mp.bmjjournals.com/
Citation
Journal Of Clinical Pathology - Molecular Pathology, 2003, v. 56 n. 1, p. 29-35 How to Cite?
AbstractAims: To characterise a strain of Gram negative aerobic straight or slightly curved rods (HKU3) isolated from the blood culture of a 9 year old Chinese boy with neutropenic fever and pseudobacteraemia. Methods: The isolate was phenotypically investigated by standard biochemical methods using conventional biochemical tests, scanning electron microscopy, and transmission electron microscopy. Genotypically, the 16S rRNA gene of the bacterium was amplified by the polymerase chain reaction (PCR) and sequenced. The sequence of the PCR product was compared with known 16S rRNA gene sequences in the Genbank by multiple sequence alignment. The G + C content was determined by thermal denaturation. A phylogenetic tree was constructed by the PileUp method. Results: The cells of the bacterial strain were aerobic, sporulating, Gram negative straight or slight curved rods. The bacterium grew on horse blood agar as non-haemolytic, grey colonies of 1 mm in diameter after 24 hours of incubation at 37°C in ambient air. No enhancement of growth was seen in 5% CO 2. It grew at 50°C as pinpoint colonies after 72 hours of incubation, but did not grow at 65°C or on MacConkey agar. It was non-motile. It produced catalase (weakly positive) and cytochrome oxidase. It reduced nitrate, produced β galactosidase, hydrolysed esculin, and utilised sodium acetate. A scanning electron micrograph of the bacterium showed straight or slightly curved rods. A transmission electron micrograph of the cell wall of the bacterium revealed multiple electron dense layers, including the outer membrane, middle murein layer, and inner cytoplasmic membrane, compatible with its Gram smear appearance. 16S rRNA gene sequencing showed that there were 7.7%, 8.0%, 8.2%, and 8.6% differences between the 16S rRNA gene sequence of the bacterium and those of Paenibacillus macerans, Paenibacillus borealis, Bacillus ehimensis, and Paenibacillus amylolyticus, respectively. The mean (SD) G + C content of the bacterium was 47.6 (2.1) mol%. Phylogenetically, it belongs to the genus paenibacillus (previously called group 3 bacillus). Conclusions: A bacterium that exhibited phenatypic and genotypic characteristics that are very different from closely related members of paenibacillus was the cause of pseudobacteraemia in a patient with neutropenic fever. A new species, Paenibacillus hongkongensis sp. nov. is proposed, for which HKU3 is the type strain.
Persistent Identifierhttp://hdl.handle.net/10722/43118
ISSN
2005 Impact Factor: 2.576
2005 SCImago Journal Rankings: 0.122
PubMed Central ID
References

 

DC FieldValueLanguage
dc.contributor.authorTeng, JLLen_HK
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorLeung, KWen_HK
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorWong, MKMen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2007-03-23T04:39:18Z-
dc.date.available2007-03-23T04:39:18Z-
dc.date.issued2003en_HK
dc.identifier.citationJournal Of Clinical Pathology - Molecular Pathology, 2003, v. 56 n. 1, p. 29-35en_HK
dc.identifier.issn1366-8714en_HK
dc.identifier.urihttp://hdl.handle.net/10722/43118-
dc.description.abstractAims: To characterise a strain of Gram negative aerobic straight or slightly curved rods (HKU3) isolated from the blood culture of a 9 year old Chinese boy with neutropenic fever and pseudobacteraemia. Methods: The isolate was phenotypically investigated by standard biochemical methods using conventional biochemical tests, scanning electron microscopy, and transmission electron microscopy. Genotypically, the 16S rRNA gene of the bacterium was amplified by the polymerase chain reaction (PCR) and sequenced. The sequence of the PCR product was compared with known 16S rRNA gene sequences in the Genbank by multiple sequence alignment. The G + C content was determined by thermal denaturation. A phylogenetic tree was constructed by the PileUp method. Results: The cells of the bacterial strain were aerobic, sporulating, Gram negative straight or slight curved rods. The bacterium grew on horse blood agar as non-haemolytic, grey colonies of 1 mm in diameter after 24 hours of incubation at 37°C in ambient air. No enhancement of growth was seen in 5% CO 2. It grew at 50°C as pinpoint colonies after 72 hours of incubation, but did not grow at 65°C or on MacConkey agar. It was non-motile. It produced catalase (weakly positive) and cytochrome oxidase. It reduced nitrate, produced β galactosidase, hydrolysed esculin, and utilised sodium acetate. A scanning electron micrograph of the bacterium showed straight or slightly curved rods. A transmission electron micrograph of the cell wall of the bacterium revealed multiple electron dense layers, including the outer membrane, middle murein layer, and inner cytoplasmic membrane, compatible with its Gram smear appearance. 16S rRNA gene sequencing showed that there were 7.7%, 8.0%, 8.2%, and 8.6% differences between the 16S rRNA gene sequence of the bacterium and those of Paenibacillus macerans, Paenibacillus borealis, Bacillus ehimensis, and Paenibacillus amylolyticus, respectively. The mean (SD) G + C content of the bacterium was 47.6 (2.1) mol%. Phylogenetically, it belongs to the genus paenibacillus (previously called group 3 bacillus). Conclusions: A bacterium that exhibited phenatypic and genotypic characteristics that are very different from closely related members of paenibacillus was the cause of pseudobacteraemia in a patient with neutropenic fever. A new species, Paenibacillus hongkongensis sp. nov. is proposed, for which HKU3 is the type strain.en_HK
dc.format.extent549538 bytes-
dc.format.extent30720 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.languageengen_HK
dc.publisherB M J Publishing Group. The Journal's web site is located at http://mp.bmjjournals.com/en_HK
dc.relation.ispartofJournal of Clinical Pathology - Molecular Pathologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsMolecular Pathology . Copyright © B M J Publishing Group.en_HK
dc.subject.meshBacillaceae - genetics - isolation & purification - ultrastructureen_HK
dc.subject.meshBacteremia - microbiologyen_HK
dc.subject.meshFever - microbiologyen_HK
dc.subject.meshNeutropenia - microbiologyen_HK
dc.subject.meshPolymerase chain reactionen_HK
dc.titlePseudobacteraemia in a patient with neutropenic fever caused by a novel paenibacillus species: Paenibacillus hongkongensis sp. nov.en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1366-8714&volume=56&issue=1&spage=29&epage=35&date=2003&atitle=Pseudobacteraemia+in+a+patient+with+neutropenic+fever+caused+by+a+novel+paenibacillus+species:+Paenibacillus+hongkongensis+sp.+nov.en_HK
dc.identifier.emailTeng, JLL:llteng@hku.hken_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityTeng, JLL=rp00277en_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1136/mp.56.1.29en_HK
dc.identifier.pmid12560460-
dc.identifier.pmcidPMC1187286-
dc.identifier.scopuseid_2-s2.0-0037312594en_HK
dc.identifier.hkuros116819-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037312594&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume56en_HK
dc.identifier.issue1en_HK
dc.identifier.spage29en_HK
dc.identifier.epage35en_HK
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridTeng, JLL=7202560229en_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridLeung, KW=7401860831en_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridWong, MKM=35123119500en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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