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Article: Clostridium bacteraemia characterised by 16S ribosomal RNA gene sequencing

TitleClostridium bacteraemia characterised by 16S ribosomal RNA gene sequencing
Authors
Issue Date2005
PublisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/
Citation
Journal Of Clinical Pathology, 2005, v. 58 n. 3, p. 301-307 How to Cite?
AbstractBackground: Owing to problems in accurate species identification of the diverse genus clostridium, the epidemiology and pathogenicity of many species are not fully understood. Moreover, previous studies on clostridium bacteraemia have been limited and relied only on phenotypic species identification. Aims: To characterise the epidemiology, disease spectrum, and outcome of clostridium bacteraemia using 16S ribosomal RNA (rRNA) gene sequencing. Method: During a four year period (1998-2001), all cases of clostridium bacteraemia were prospectively studied and all "non-perfringens" clostridium isolates identified to the species level by 16S rRNA gene sequencing. Results: Fifty one blood culture isolates were identified as Clostridium perfringens and 17 belonged to 11 other clostridium species. The first case of C disporicum infection and two cases of clostridium bacteraemia in children with intussusception were also described. Of the 68 clostridium isolates from 68 different patients, 38 were associated with clinically relevant bacteraemia. The gastrointestinal and hepatobiliary tracts were common sites of both underlying disease and portal of entry in these patients. Clostridium perfringens accounted for 79% of all clinically relevant bacteraemia, with the remainder caused by a diversity of species. The attributable mortality rate of clinically relevant clostridium bacteraemia was 299%. Younger age and underlying gastrointestinal/hepatobiliary tract disease were associated with mortality (p < 0.05). Conclusions: Patients with clinically relevant clostridium bacteraemia should be investigated for the presence of underlying disease processes in the gastrointestinal or hepatobiliary tracts. 16S rRNA gene analysis will continue to be useful in further understanding the pathogenicity of various clostridium species.
Persistent Identifierhttp://hdl.handle.net/10722/43115
ISSN
2014 Impact Factor: 2.915
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorChan, KMen_HK
dc.contributor.authorFung, AMYen_HK
dc.contributor.authorTang, BSFen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2007-03-23T04:39:13Z-
dc.date.available2007-03-23T04:39:13Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Clinical Pathology, 2005, v. 58 n. 3, p. 301-307en_HK
dc.identifier.issn0021-9746en_HK
dc.identifier.urihttp://hdl.handle.net/10722/43115-
dc.description.abstractBackground: Owing to problems in accurate species identification of the diverse genus clostridium, the epidemiology and pathogenicity of many species are not fully understood. Moreover, previous studies on clostridium bacteraemia have been limited and relied only on phenotypic species identification. Aims: To characterise the epidemiology, disease spectrum, and outcome of clostridium bacteraemia using 16S ribosomal RNA (rRNA) gene sequencing. Method: During a four year period (1998-2001), all cases of clostridium bacteraemia were prospectively studied and all "non-perfringens" clostridium isolates identified to the species level by 16S rRNA gene sequencing. Results: Fifty one blood culture isolates were identified as Clostridium perfringens and 17 belonged to 11 other clostridium species. The first case of C disporicum infection and two cases of clostridium bacteraemia in children with intussusception were also described. Of the 68 clostridium isolates from 68 different patients, 38 were associated with clinically relevant bacteraemia. The gastrointestinal and hepatobiliary tracts were common sites of both underlying disease and portal of entry in these patients. Clostridium perfringens accounted for 79% of all clinically relevant bacteraemia, with the remainder caused by a diversity of species. The attributable mortality rate of clinically relevant clostridium bacteraemia was 299%. Younger age and underlying gastrointestinal/hepatobiliary tract disease were associated with mortality (p < 0.05). Conclusions: Patients with clinically relevant clostridium bacteraemia should be investigated for the presence of underlying disease processes in the gastrointestinal or hepatobiliary tracts. 16S rRNA gene analysis will continue to be useful in further understanding the pathogenicity of various clostridium species.en_HK
dc.format.extent108009 bytes-
dc.format.extent30720 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.languageengen_HK
dc.publisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/en_HK
dc.relation.ispartofJournal of Clinical Pathologyen_HK
dc.rightsJournal of clinical pathology. Copyright © B M J Publishing Group.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshBacteremia - diagnosis - epidemiology - microbiologyen_HK
dc.subject.meshClostridium - classification - isolation & purification - pathogenicityen_HK
dc.subject.meshClostridium infections - diagnosis - epidemiology - microbiologyen_HK
dc.subject.meshRna, bacterial - geneticsen_HK
dc.subject.meshRna, ribosomal, 16s - geneticsen_HK
dc.titleClostridium bacteraemia characterised by 16S ribosomal RNA gene sequencingen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9746&volume=58&issue=3&spage=301&epage=307&date=2005&atitle=Clostridium+bacteraemia+characterised+by+16S+ribosomal+RNA+gene+sequencingen_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1136/jcp.2004.022830en_HK
dc.identifier.pmid15735165-
dc.identifier.pmcidPMC1770585-
dc.identifier.scopuseid_2-s2.0-15044349638en_HK
dc.identifier.hkuros100305-
dc.identifier.hkuros114665-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-15044349638&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume58en_HK
dc.identifier.issue3en_HK
dc.identifier.spage301en_HK
dc.identifier.epage307en_HK
dc.identifier.isiWOS:000227691100015-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridChan, KM=7406034671en_HK
dc.identifier.scopusauthoridFung, AMY=7101926801en_HK
dc.identifier.scopusauthoridTang, BSF=8908243000en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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