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Article: Detection of CMV DNA in bone marrow transplant recipients: Plasma versus leucocyte polymerase chain reaction

TitleDetection of CMV DNA in bone marrow transplant recipients: Plasma versus leucocyte polymerase chain reaction
Authors
Issue Date1997
PublisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/
Citation
Journal Of Clinical Pathology, 1997, v. 50 n. 3, p. 231-235 How to Cite?
AbstractAims - To compare the nested polymerase chain reaction (PCR) assay for the detection of cytomegalovirus (CMV) DNA in peripheral blood leucocytes and plasma obtained from heparinised blood; to determine the efficiency of various DNA extraction methods to minimise inhibition of plasma PCR and their effect on the sensitivity of plasma PCR; to determine the inhibitory effect of heparin, dextran, and EDTA on the CMV PCR assay. Methods - 217 heparinised blood specimens from 58 bone marrow transplant patients were processed and the sensitivities and specificities of the PCR assays using peripheral blood leucocytes and plasma (with simple, Instagene, and Geneclean extraction methods) were compared to those of conventional CMV culture. In a separate experiment, dilutions of heparin, dextran, and EDTA were included in PCR assays. Results - The detection of CMV DNA using peripheral blood leucocytes for PCR assay was significantly more sensitive (100%) than when using plasma (60%). Instagene and Geneclean extraction removed inherent inhibition but did not improve the sensitivity of the plasma PCR reaction. Heparin had an inhibitory effect on PCR. Conclusions - PCR assay using peripheral blood leucocytes is better than plasma for guiding the prescription of ganciclovir to bone marrow transplant patients. Heparin is inhibitory to the plasma PCR reaction.
Persistent Identifierhttp://hdl.handle.net/10722/43084
ISSN
2015 Impact Factor: 2.912
2015 SCImago Journal Rankings: 1.260
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorLo, SKFen_HK
dc.contributor.authorYuen, KYen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.contributor.authorSiau, Hen_HK
dc.contributor.authorChiu, EKWen_HK
dc.contributor.authorLiang, RHSen_HK
dc.contributor.authorChan, TKen_HK
dc.date.accessioned2007-03-23T04:38:31Z-
dc.date.available2007-03-23T04:38:31Z-
dc.date.issued1997en_HK
dc.identifier.citationJournal Of Clinical Pathology, 1997, v. 50 n. 3, p. 231-235en_HK
dc.identifier.issn0021-9746en_HK
dc.identifier.urihttp://hdl.handle.net/10722/43084-
dc.description.abstractAims - To compare the nested polymerase chain reaction (PCR) assay for the detection of cytomegalovirus (CMV) DNA in peripheral blood leucocytes and plasma obtained from heparinised blood; to determine the efficiency of various DNA extraction methods to minimise inhibition of plasma PCR and their effect on the sensitivity of plasma PCR; to determine the inhibitory effect of heparin, dextran, and EDTA on the CMV PCR assay. Methods - 217 heparinised blood specimens from 58 bone marrow transplant patients were processed and the sensitivities and specificities of the PCR assays using peripheral blood leucocytes and plasma (with simple, Instagene, and Geneclean extraction methods) were compared to those of conventional CMV culture. In a separate experiment, dilutions of heparin, dextran, and EDTA were included in PCR assays. Results - The detection of CMV DNA using peripheral blood leucocytes for PCR assay was significantly more sensitive (100%) than when using plasma (60%). Instagene and Geneclean extraction removed inherent inhibition but did not improve the sensitivity of the plasma PCR reaction. Heparin had an inhibitory effect on PCR. Conclusions - PCR assay using peripheral blood leucocytes is better than plasma for guiding the prescription of ganciclovir to bone marrow transplant patients. Heparin is inhibitory to the plasma PCR reaction.en_HK
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dc.format.mimetypeapplication/pdf-
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dc.languageengen_HK
dc.publisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/en_HK
dc.relation.ispartofJournal of Clinical Pathologyen_HK
dc.rightsJournal of Clinical Pathology. Copyright © B M J Publishing Group.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshBone marrow transplantationen_HK
dc.subject.meshCytomegalovirus - genetics - isolation & purificationen_HK
dc.subject.meshLeukocytes - virologyen_HK
dc.subject.meshVirology - methods - standardsen_HK
dc.subject.meshSensitivity and specificityen_HK
dc.titleDetection of CMV DNA in bone marrow transplant recipients: Plasma versus leucocyte polymerase chain reactionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9746&volume=50&issue=3&spage=231&epage=235&date=1997&atitle=Detection+of+CMV+DNA+in+bone+marrow+transplant+recipients:+plasma+versus+leucocyte+polymerase+chain+reactionen_HK
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.emailLiang, RHS: rliang@hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.identifier.authorityLiang, RHS=rp00345en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid9155674-
dc.identifier.pmcidPMC499818-
dc.identifier.scopuseid_2-s2.0-0030959836en_HK
dc.identifier.hkuros36222-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030959836&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume50en_HK
dc.identifier.issue3en_HK
dc.identifier.spage231en_HK
dc.identifier.epage235en_HK
dc.identifier.isiWOS:A1997WQ72400011-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridLo, SKF=7401542391en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.scopusauthoridSiau, H=16751930400en_HK
dc.identifier.scopusauthoridChiu, EKW=24827833600en_HK
dc.identifier.scopusauthoridLiang, RHS=26643224900en_HK
dc.identifier.scopusauthoridChan, TK=37076590700en_HK

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