Article: Tracking down the migration of mouse neural crest cells

File Download Links for fulltext
(May Require Subscription)
Supplementary
  • Basic View
  • Metadata View
  • XML View
TitleTracking down the migration of mouse neural crest cells
AuthorsChan, WY3
Tam, WY3
Yung, KM3
Cheung, CS3
Sham, MH1
Copp, AJ2
KeywordsCell markers
DiI
Electroporation
Green fluorescent protein
Hoxb2-lacZ
Microinjection
Mouse embryo
Wheat germ agglutinin-gold conjugate
Whole embryo culture
Issue Date2003
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/nba
CitationNeuroembryology, 2003, v. 2 n. 1, p. 9-17 [How to Cite?]
DOI: http://dx.doi.org/10.1159/000068497
AbstractDuring early embryonic development, cell migration is one of the most important morphogenetic processes. Neural crest cells arise from the dorsal part of the neural tube and migrate along different pathways to numerous locations where they differentiate into a variety of tissues. In the mouse, studies of neural crest cell migration have been difficult partly because of the absence of specific markers which can label neural crest cells throughout their migration from their origin to the site of differentiation. Nevertheless, the use of different experimental strategies involving extrinsic, intrinsic or genetic cell markers has already led to a good understanding of this migration. In our studies, extrinsic markers such as wheat germ agglutinin-gold conjugates and DiI and genetic markers including Hoxb2-lacZ and green fluorescent protein have been employed in tracing migrating neural crest cells. The labelling procedures and the strength and weaknesses of the tracing methods are reviewed herein. Copyright © 2003 S. Karger AG, Basel.
ISSN1424-8522
DOIhttp://dx.doi.org/10.1159/000068497
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorChan, WY
dc.contributor.authorTam, WY
dc.contributor.authorYung, KM
dc.contributor.authorCheung, CS
dc.contributor.authorSham, MH
dc.contributor.authorCopp, AJ
dc.date.accessioned2007-03-23T04:27:57Z
dc.date.available2007-03-23T04:27:57Z
dc.date.issued2003
dc.description.abstractDuring early embryonic development, cell migration is one of the most important morphogenetic processes. Neural crest cells arise from the dorsal part of the neural tube and migrate along different pathways to numerous locations where they differentiate into a variety of tissues. In the mouse, studies of neural crest cell migration have been difficult partly because of the absence of specific markers which can label neural crest cells throughout their migration from their origin to the site of differentiation. Nevertheless, the use of different experimental strategies involving extrinsic, intrinsic or genetic cell markers has already led to a good understanding of this migration. In our studies, extrinsic markers such as wheat germ agglutinin-gold conjugates and DiI and genetic markers including Hoxb2-lacZ and green fluorescent protein have been employed in tracing migrating neural crest cells. The labelling procedures and the strength and weaknesses of the tracing methods are reviewed herein. Copyright © 2003 S. Karger AG, Basel.
dc.description.naturepublished_or_final_version
dc.format.extent177927 bytes
dc.format.extent25600 bytes
dc.format.extent1791017 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/msword
dc.format.mimetypeapplication/pdf
dc.identifier.citationNeuroembryology, 2003, v. 2 n. 1, p. 9-17 [How to Cite?]
DOI: http://dx.doi.org/10.1159/000068497
dc.identifier.doihttp://dx.doi.org/10.1159/000068497
dc.identifier.epage17
dc.identifier.hkuros84265
dc.identifier.issn1424-8522
dc.identifier.issue1
dc.identifier.openurl
dc.identifier.scopuseid_2-s2.0-0344011111
dc.identifier.spage9
dc.identifier.urihttp://hdl.handle.net/10722/42617
dc.identifier.volume2
dc.languageeng
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/nba
dc.publisher.placeSwitzerland
dc.relation.ispartofNeuroembryology
dc.relation.referencesReferences in Scopus
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
dc.subjectCell markers
dc.subjectDiI
dc.subjectElectroporation
dc.subjectGreen fluorescent protein
dc.subjectHoxb2-lacZ
dc.subjectMicroinjection
dc.subjectMouse embryo
dc.subjectWheat germ agglutinin-gold conjugate
dc.subjectWhole embryo culture
dc.titleTracking down the migration of mouse neural crest cells
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. UCL Institute of Child Health
  3. Chinese University of Hong Kong