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Article: Tracking down the migration of mouse neural crest cells
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TitleTracking down the migration of mouse neural crest cells
 
AuthorsChan, WY3
Tam, WY3
Yung, KM3
Cheung, CS3
Sham, MH1
Copp, AJ2
 
KeywordsCell markers
DiI
Electroporation
Green fluorescent protein
Hoxb2-lacZ
Microinjection
Mouse embryo
Wheat germ agglutinin-gold conjugate
Whole embryo culture
 
Issue Date2003
 
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/nba
 
CitationNeuroembryology, 2003, v. 2 n. 1, p. 9-17 [How to Cite?]
DOI: http://dx.doi.org/10.1159/000068497
 
AbstractDuring early embryonic development, cell migration is one of the most important morphogenetic processes. Neural crest cells arise from the dorsal part of the neural tube and migrate along different pathways to numerous locations where they differentiate into a variety of tissues. In the mouse, studies of neural crest cell migration have been difficult partly because of the absence of specific markers which can label neural crest cells throughout their migration from their origin to the site of differentiation. Nevertheless, the use of different experimental strategies involving extrinsic, intrinsic or genetic cell markers has already led to a good understanding of this migration. In our studies, extrinsic markers such as wheat germ agglutinin-gold conjugates and DiI and genetic markers including Hoxb2-lacZ and green fluorescent protein have been employed in tracing migrating neural crest cells. The labelling procedures and the strength and weaknesses of the tracing methods are reviewed herein. Copyright © 2003 S. Karger AG, Basel.
 
ISSN1424-8522
 
DOIhttp://dx.doi.org/10.1159/000068497
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorChan, WY
 
dc.contributor.authorTam, WY
 
dc.contributor.authorYung, KM
 
dc.contributor.authorCheung, CS
 
dc.contributor.authorSham, MH
 
dc.contributor.authorCopp, AJ
 
dc.date.accessioned2007-03-23T04:27:57Z
 
dc.date.available2007-03-23T04:27:57Z
 
dc.date.issued2003
 
dc.description.abstractDuring early embryonic development, cell migration is one of the most important morphogenetic processes. Neural crest cells arise from the dorsal part of the neural tube and migrate along different pathways to numerous locations where they differentiate into a variety of tissues. In the mouse, studies of neural crest cell migration have been difficult partly because of the absence of specific markers which can label neural crest cells throughout their migration from their origin to the site of differentiation. Nevertheless, the use of different experimental strategies involving extrinsic, intrinsic or genetic cell markers has already led to a good understanding of this migration. In our studies, extrinsic markers such as wheat germ agglutinin-gold conjugates and DiI and genetic markers including Hoxb2-lacZ and green fluorescent protein have been employed in tracing migrating neural crest cells. The labelling procedures and the strength and weaknesses of the tracing methods are reviewed herein. Copyright © 2003 S. Karger AG, Basel.
 
dc.description.naturepublished_or_final_version
 
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dc.format.extent25600 bytes
 
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dc.identifier.citationNeuroembryology, 2003, v. 2 n. 1, p. 9-17 [How to Cite?]
DOI: http://dx.doi.org/10.1159/000068497
 
dc.identifier.doihttp://dx.doi.org/10.1159/000068497
 
dc.identifier.epage17
 
dc.identifier.hkuros84265
 
dc.identifier.issn1424-8522
 
dc.identifier.issue1
 
dc.identifier.openurl
 
dc.identifier.scopuseid_2-s2.0-0344011111
 
dc.identifier.spage9
 
dc.identifier.urihttp://hdl.handle.net/10722/42617
 
dc.identifier.volume2
 
dc.languageeng
 
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/nba
 
dc.publisher.placeSwitzerland
 
dc.relation.ispartofNeuroembryology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subjectCell markers
 
dc.subjectDiI
 
dc.subjectElectroporation
 
dc.subjectGreen fluorescent protein
 
dc.subjectHoxb2-lacZ
 
dc.subjectMicroinjection
 
dc.subjectMouse embryo
 
dc.subjectWheat germ agglutinin-gold conjugate
 
dc.subjectWhole embryo culture
 
dc.titleTracking down the migration of mouse neural crest cells
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. UCL Institute of Child Health
  3. Chinese University of Hong Kong