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Article: Rapid in vivo Taxotere quantitative chemosensitivity response by 4.23 Tesla sodium MRI and histo-immunostaining features in N-Methyl-N-Nitrosourea induced breast tumors in rats

TitleRapid in vivo Taxotere quantitative chemosensitivity response by 4.23 Tesla sodium MRI and histo-immunostaining features in N-Methyl-N-Nitrosourea induced breast tumors in rats
Authors
KeywordsAntineoplastics
Apoptosis
Non-invasive
Taxotere
Therapeutic efficacy
Issue Date2005
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.cancerci.com
Citation
Cancer Cell International, 2005, v. 5 How to Cite?
AbstractBackground: Sodium weighted images can indicate sodium signal intensities from different features in the tumor before and 24 hours following administration of Taxotere. Aim: To evaluate the association of in vivo intracellular sodium magnetic resonance image intensities with immuno-biomarkers and histopathological features to monitor the early tumor response to Taxotere chemotherapy in Methyl-Nitroso-Urea induced rat xenograft breast tumors. Methods and Materials: Methyl-Nitroso-Urea (MNU) induced rat xenograft breast tumors were imaged for sodium MRI and compared with tumor histology, immunostaining after 24 hours chemotherapy. Results: Sodium MRI signal intensities represented sodium concentrations. Excised tumor histological sections showed different in vitro histological end points i.e. single strand DNA content of cell nuclei during cell cycle (G1/S-G2/M), distinct S or M histograms (Feulgen labeling to nuclear DNA content by CAS 200), mitotic figures and apoptosis at different locations of breast tumors. Necrosis and cystic fluid appeared gray on intracellular (IC) sodium images while apoptosis rich regions appeared brighter on IC sodium images. After 24 hours Taxotere-treated tumors showed lower 'IC/EC ratio' of viable cells (65-76%) with higher mitotic index; apoptotic tumor cells at high risk due to cytotoxicity (>70% with high apoptotic index); reduced proliferation index (270 vs 120 per high power field) associated with enhanced IC sodium in vivo MR image intensities and decreased tumor size (3%; p < 0.001; n = 16) than that of pre-treated tumors. IC-Na MR signal intensities possibly indicated Taxotere chemosensitivity response in vivo associated with apoptosis and different pre-malignant features within 24 hours of exposure of cancer cells to anti-neoplastic Taxotere drug. Conclusion: Sodium MRI imaging may be used as in vivo rapid drug monitoring method to evaluate Taxotere chemosensitivity response associated with neoplasia, apoptosis and tumor histology features. © 2005 Sharma et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/42520
ISSN
2015 Impact Factor: 2.884
2015 SCImago Journal Rankings: 1.050
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSharma, Ren_HK
dc.contributor.authorKline, RPen_HK
dc.contributor.authorWu, EXen_HK
dc.contributor.authorKatz, JKen_HK
dc.date.accessioned2007-01-29T08:51:58Z-
dc.date.available2007-01-29T08:51:58Z-
dc.date.issued2005en_HK
dc.identifier.citationCancer Cell International, 2005, v. 5en_HK
dc.identifier.issn1475-2867en_HK
dc.identifier.urihttp://hdl.handle.net/10722/42520-
dc.description.abstractBackground: Sodium weighted images can indicate sodium signal intensities from different features in the tumor before and 24 hours following administration of Taxotere. Aim: To evaluate the association of in vivo intracellular sodium magnetic resonance image intensities with immuno-biomarkers and histopathological features to monitor the early tumor response to Taxotere chemotherapy in Methyl-Nitroso-Urea induced rat xenograft breast tumors. Methods and Materials: Methyl-Nitroso-Urea (MNU) induced rat xenograft breast tumors were imaged for sodium MRI and compared with tumor histology, immunostaining after 24 hours chemotherapy. Results: Sodium MRI signal intensities represented sodium concentrations. Excised tumor histological sections showed different in vitro histological end points i.e. single strand DNA content of cell nuclei during cell cycle (G1/S-G2/M), distinct S or M histograms (Feulgen labeling to nuclear DNA content by CAS 200), mitotic figures and apoptosis at different locations of breast tumors. Necrosis and cystic fluid appeared gray on intracellular (IC) sodium images while apoptosis rich regions appeared brighter on IC sodium images. After 24 hours Taxotere-treated tumors showed lower 'IC/EC ratio' of viable cells (65-76%) with higher mitotic index; apoptotic tumor cells at high risk due to cytotoxicity (>70% with high apoptotic index); reduced proliferation index (270 vs 120 per high power field) associated with enhanced IC sodium in vivo MR image intensities and decreased tumor size (3%; p < 0.001; n = 16) than that of pre-treated tumors. IC-Na MR signal intensities possibly indicated Taxotere chemosensitivity response in vivo associated with apoptosis and different pre-malignant features within 24 hours of exposure of cancer cells to anti-neoplastic Taxotere drug. Conclusion: Sodium MRI imaging may be used as in vivo rapid drug monitoring method to evaluate Taxotere chemosensitivity response associated with neoplasia, apoptosis and tumor histology features. © 2005 Sharma et al; licensee BioMed Central Ltd.en_HK
dc.format.extent1497990 bytes-
dc.format.extent25088 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.languageengen_HK
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.cancerci.comen_HK
dc.relation.ispartofCancer Cell Internationalen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectAntineoplasticsen_HK
dc.subjectApoptosisen_HK
dc.subjectNon-invasiveen_HK
dc.subjectTaxotereen_HK
dc.subjectTherapeutic efficacyen_HK
dc.titleRapid in vivo Taxotere quantitative chemosensitivity response by 4.23 Tesla sodium MRI and histo-immunostaining features in N-Methyl-N-Nitrosourea induced breast tumors in ratsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1475-2867&volume=5&issue=1&spage=26&epage=&date=2005&atitle=Rapid+in+vivo+Taxotere+quantitative+chemosensitivity+response+by+4.23+Tesla+sodium+MRI+and+histo-immunostaining+features+in+N-Methyl-N-Nitrosourea+induced+breast+tumors+in+ratsen_HK
dc.identifier.emailWu, EX:ewu1@hkucc.hku.hken_HK
dc.identifier.authorityWu, EX=rp00193en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1186/1475-2867-5-26en_HK
dc.identifier.pmid16078994en_HK
dc.identifier.pmcidPMC1208921-
dc.identifier.scopuseid_2-s2.0-26844479552en_HK
dc.identifier.hkuros119800-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-26844479552&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume5en_HK
dc.identifier.isiWOS:000208404900026-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridSharma, R=7407242763en_HK
dc.identifier.scopusauthoridKline, RP=7103055795en_HK
dc.identifier.scopusauthoridWu, EX=7202128034en_HK
dc.identifier.scopusauthoridKatz, JK=7403968068en_HK

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