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Article: Effect of Helicobacter pylori on apoptosis and apoptosis related genes in gastric cancer cells

TitleEffect of Helicobacter pylori on apoptosis and apoptosis related genes in gastric cancer cells
Authors
Issue Date2003
PublisherB M J Publishing Group. The Journal's web site is located at http://mp.bmjjournals.com/
Citation
Journal Of Clinical Pathology - Molecular Pathology, 2003, v. 56 n. 1, p. 19-24 How to Cite?
AbstractBackground/Aims: Helicobacter pylori induces the apoptosis of gastric epithelial cells in vivo and in vitro. However, the molecular mechanism has not been clarified. The aim of this study was to investigate the effect of H pylori on the apoptosis of gastric epithelial cells and the expression of apoptosis related genes in vitro. Methods: Human gastric adenocarcinoma SGC-7901 cells were co-cultured with a cytotoxic H pylori strain, NCTC 11637, at various densities ranging from 3.2 × 104 to 1.0 × 108 colony forming units (CFU)/ml for 48 hours. Apoptosis in gastric cells was determined by transmission electron microscopy, Hoechst 33258 fluorochrome staining, and flow cytometry. The expression of apoptosis related proteins, Bcl-2, Bax, and c-Myc, was measured by an immunohistochemical method, and c-Myc mRNA expression was determined by the reverse transcription-polymerase chain reaction. Results: Helicobacter pylori induces morphological changes typical of apoptosis. Both fluorochrome staining and flow cytometry showed that the apoptotic index began to increase when H pylori were at a density of > 1.6 × 104 CFU/ml, and in a density dependent manner (p < 0.01; one way ANOVA). The expression of the Bax and c-Myc proteins and of c-Myc mRNA was increased, whereas Bcl-2 expression was decreased after co-culture for 48 hours. Conclusions: Helicobacter pylori induced apoptosis in gastric epithelial cells is mediated by altered expression of the products of the Bcl-2, Bax, and c-Myc genes.
Persistent Identifierhttp://hdl.handle.net/10722/42421
ISSN
2005 Impact Factor: 2.576
2005 SCImago Journal Rankings: 0.122
PubMed Central ID
References

 

DC FieldValueLanguage
dc.contributor.authorYang, Yen_HK
dc.contributor.authorDeng, CSen_HK
dc.contributor.authorPeng, JZen_HK
dc.contributor.authorWong, BCYen_HK
dc.contributor.authorLam, SKen_HK
dc.contributor.authorXia, HHXen_HK
dc.date.accessioned2007-01-29T08:49:34Z-
dc.date.available2007-01-29T08:49:34Z-
dc.date.issued2003en_HK
dc.identifier.citationJournal Of Clinical Pathology - Molecular Pathology, 2003, v. 56 n. 1, p. 19-24en_HK
dc.identifier.issn1366-8714en_HK
dc.identifier.urihttp://hdl.handle.net/10722/42421-
dc.description.abstractBackground/Aims: Helicobacter pylori induces the apoptosis of gastric epithelial cells in vivo and in vitro. However, the molecular mechanism has not been clarified. The aim of this study was to investigate the effect of H pylori on the apoptosis of gastric epithelial cells and the expression of apoptosis related genes in vitro. Methods: Human gastric adenocarcinoma SGC-7901 cells were co-cultured with a cytotoxic H pylori strain, NCTC 11637, at various densities ranging from 3.2 × 104 to 1.0 × 108 colony forming units (CFU)/ml for 48 hours. Apoptosis in gastric cells was determined by transmission electron microscopy, Hoechst 33258 fluorochrome staining, and flow cytometry. The expression of apoptosis related proteins, Bcl-2, Bax, and c-Myc, was measured by an immunohistochemical method, and c-Myc mRNA expression was determined by the reverse transcription-polymerase chain reaction. Results: Helicobacter pylori induces morphological changes typical of apoptosis. Both fluorochrome staining and flow cytometry showed that the apoptotic index began to increase when H pylori were at a density of > 1.6 × 104 CFU/ml, and in a density dependent manner (p < 0.01; one way ANOVA). The expression of the Bax and c-Myc proteins and of c-Myc mRNA was increased, whereas Bcl-2 expression was decreased after co-culture for 48 hours. Conclusions: Helicobacter pylori induced apoptosis in gastric epithelial cells is mediated by altered expression of the products of the Bcl-2, Bax, and c-Myc genes.en_HK
dc.format.extent189394 bytes-
dc.format.extent25600 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.languageengen_HK
dc.publisherB M J Publishing Group. The Journal's web site is located at http://mp.bmjjournals.com/en_HK
dc.relation.ispartofJournal of Clinical Pathology - Molecular Pathologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsMolecular Pathology. Copyright © B M J Publishing Group.en_HK
dc.subject.meshAdenocarcinoma - genetics - microbiology - ultrastructureen_HK
dc.subject.meshApoptosis - geneticsen_HK
dc.subject.meshElectrophoresis, polyacrylamide gel - methodsen_HK
dc.subject.meshGenes, neoplasm - geneticsen_HK
dc.subject.meshStomach neoplasms - genetics - microbiology - ultrastructureen_HK
dc.titleEffect of Helicobacter pylori on apoptosis and apoptosis related genes in gastric cancer cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1366-8714&volume=56&issue=1&spage=19&epage=24&date=2003&atitle=Effect+of+Helicobacter+pylori+on+apoptosis+and+apoptosis+related+genes+in+gastric+cancer+cellsen_HK
dc.identifier.emailWong, BCY:bcywong@hku.hken_HK
dc.identifier.authorityWong, BCY=rp00429en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1136/mp.56.1.19en_HK
dc.identifier.pmid12560457-
dc.identifier.pmcidPMC1187283-
dc.identifier.scopuseid_2-s2.0-0043064185en_HK
dc.identifier.hkuros82286-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0043064185&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume56en_HK
dc.identifier.issue1en_HK
dc.identifier.spage19en_HK
dc.identifier.epage24en_HK
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYang, Y=8675011000en_HK
dc.identifier.scopusauthoridDeng, CS=7202303331en_HK
dc.identifier.scopusauthoridPeng, JZ=12796973500en_HK
dc.identifier.scopusauthoridWong, BCY=7402023340en_HK
dc.identifier.scopusauthoridLam, SK=7402279473en_HK
dc.identifier.scopusauthoridXia, HHX=8757161400en_HK

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