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Article: Derivative chromosome 9 deletions in chronic myeloid leukaemia: Interpretation of atypical D-FISH pattern

TitleDerivative chromosome 9 deletions in chronic myeloid leukaemia: Interpretation of atypical D-FISH pattern
Authors
Issue Date2003
PublisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/
Citation
Journal Of Clinical Pathology, 2003, v. 56 n. 6, p. 471-474 How to Cite?
AbstractBackground/Aims: New molecular cytogenetic techniques are increasingly applied as a routine investigative tool in haematological malignancies, both at diagnosis and subsequent monitoring. This report describes the interpretation of atypical signal patterns encountered using BCR-ABL dual colour dual fusion fluorescence in situ hybridisation (D-FISH) translocation probes in chronic myeloid leukaemia (CML). Methods: Interphase FISH experiments were carried out using BCR-ABL D-FISH probes in 46 patients with CML at diagnosis and during subsequent disease monitoring. Atypical hybridisation signal patterns were characterised by molecular cytogenetic techniques and correlated with conventional karyotyping. Results: Two patients showed atypical interphase D-FISH patterns with one orange, one green, and one fusion (1O1G1F) signal. The presence of BCR-ABL gene fusion was documented by a dual colour single fusion (S-FISH) probe. The submicroscopic deletion of the ABL-BCR fusion gene on the derivative chromosome 9 in these cases was subsequently characterised by metaphase FISH on relocated G banded metaphases. Conclusions: Atypical interphase D-FISH patterns should not be interpreted in isolation and should be considered in conjunction with other cytogenetic or molecular genetic investigations.
Persistent Identifierhttp://hdl.handle.net/10722/42420
ISSN
2015 Impact Factor: 2.912
2015 SCImago Journal Rankings: 1.260
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWan, TSKen_HK
dc.contributor.authorMa, SKen_HK
dc.contributor.authorAu, WYen_HK
dc.contributor.authorChan, LCen_HK
dc.date.accessioned2007-01-29T08:49:33Z-
dc.date.available2007-01-29T08:49:33Z-
dc.date.issued2003en_HK
dc.identifier.citationJournal Of Clinical Pathology, 2003, v. 56 n. 6, p. 471-474en_HK
dc.identifier.issn0021-9746en_HK
dc.identifier.urihttp://hdl.handle.net/10722/42420-
dc.description.abstractBackground/Aims: New molecular cytogenetic techniques are increasingly applied as a routine investigative tool in haematological malignancies, both at diagnosis and subsequent monitoring. This report describes the interpretation of atypical signal patterns encountered using BCR-ABL dual colour dual fusion fluorescence in situ hybridisation (D-FISH) translocation probes in chronic myeloid leukaemia (CML). Methods: Interphase FISH experiments were carried out using BCR-ABL D-FISH probes in 46 patients with CML at diagnosis and during subsequent disease monitoring. Atypical hybridisation signal patterns were characterised by molecular cytogenetic techniques and correlated with conventional karyotyping. Results: Two patients showed atypical interphase D-FISH patterns with one orange, one green, and one fusion (1O1G1F) signal. The presence of BCR-ABL gene fusion was documented by a dual colour single fusion (S-FISH) probe. The submicroscopic deletion of the ABL-BCR fusion gene on the derivative chromosome 9 in these cases was subsequently characterised by metaphase FISH on relocated G banded metaphases. Conclusions: Atypical interphase D-FISH patterns should not be interpreted in isolation and should be considered in conjunction with other cytogenetic or molecular genetic investigations.en_HK
dc.format.extent119427 bytes-
dc.format.extent25600 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.languageengen_HK
dc.publisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/en_HK
dc.relation.ispartofJournal of Clinical Pathologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Clinical Pathology. Copyright © B M J Publishing Group.en_HK
dc.subject.meshChromosome deletionen_HK
dc.subject.meshChromosomes, human, pair 9 - geneticsen_HK
dc.subject.meshFusion proteins, bcr-ablen_HK
dc.subject.meshIn situ hybridization, fluorescenceen_HK
dc.subject.meshLeukemia, myeloid, chronicen_HK
dc.titleDerivative chromosome 9 deletions in chronic myeloid leukaemia: Interpretation of atypical D-FISH patternen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9746&volume=56&issue=6&spage=471&epage=474&date=2003&atitle=Derivative+chromosome+9+deletions+in+chronic+myeloid+leukaemia:+interpretation+of+atypical+D-FISH+patternen_HK
dc.identifier.emailChan, LC:chanlc@hkucc.hku.hken_HK
dc.identifier.authorityChan, LC=rp00373en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1136/jcp.56.6.471en_HK
dc.identifier.pmid12783977-
dc.identifier.pmcidPMC1769964-
dc.identifier.scopuseid_2-s2.0-0038309575en_HK
dc.identifier.hkuros76745-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0038309575&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume56en_HK
dc.identifier.issue6en_HK
dc.identifier.spage471en_HK
dc.identifier.epage474en_HK
dc.identifier.isiWOS:000183280700015-
dc.publisher.placeUnited Kingdomen_HK

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