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Article: Axonal regeneration of retinal ganglion cells after optic nerve pre-lesions and attachment of normal or pre-degenerated peripheral nerve grafts

TitleAxonal regeneration of retinal ganglion cells after optic nerve pre-lesions and attachment of normal or pre-degenerated peripheral nerve grafts
Authors
KeywordsOptic nerve
Pre-lesion
Regeneration
Retinal ganglion cell
Transplantation
Issue Date2002
PublisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=VNS
Citation
Visual Neuroscience, 2002, v. 19 n. 5, p. 661-668 How to Cite?
AbstractAxonal regeneration of retinal ganglion cells (RGCs) into a normal or pre-degenerated peripheral nerve graft after an optic nerve pre-lesion was investigated. A pre-lesion performed 1-2 weeks before a second lesion has been shown to enhance axonal regeneration in peripheral nerves (PN) but not in optic nerves (ON) in mammals. The lack of such a beneficial pre-lesion effect may be due to the long delay (1-6 weeks) between the two lesions since RGCs and their axons degenerate rapidly 1-2 weeks following axotomy in adult rodents. The present study examined the effects of the proximal and distal ON pre-lesions with a shortened delay (0-8 days) on axonal regeneration of RGCs through a normal or pre-degenerated PN graft. The ON of adult hamsters was transected intraorbitally at 2 mm (proximal lesion) or intracranially at 7 mm (distal lesion) from the optic disc. The pre-lesioned ON was re-transected at 0.5 mm from the disc after 0, 1, 2, 4, or 8 days and a normal or a pre-degenerated PN graft was attached onto the ocular stump. The number of RGCs regenerating their injured axons into the PN graft was estimated by retrograde labeling with FluoroGold 4 weeks after grafting. The number of regenerating RGCs decreased significantly when the delay-time increased in animals with both the ON pre-lesions (proximal or distal) compared to control animals without an ON pre-lesion. The proximal ON pre-lesion significantly reduced the number of regenerating RGCs after a delay of 8 days in comparison with the distal lesion. However, this adverse effect can be overcome, to some degree, by a pre-degenerated PN graft applied 2, 4, or 8 days after the distal ON pre-lesion enhanced more RGCs to regenerate than the normal PN graft. Thus, in order to obtain the highest number of regenerating RGCs, a pre-degenerated PN should be grafted immediately after an ON lesion.
Persistent Identifierhttp://hdl.handle.net/10722/42335
ISSN
2015 Impact Factor: 1.871
2015 SCImago Journal Rankings: 1.231
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYou, SWen_HK
dc.contributor.authorBedi, KSen_HK
dc.contributor.authorYip, HKen_HK
dc.contributor.authorSo, KFen_HK
dc.date.accessioned2007-01-29T08:47:09Z-
dc.date.available2007-01-29T08:47:09Z-
dc.date.issued2002en_HK
dc.identifier.citationVisual Neuroscience, 2002, v. 19 n. 5, p. 661-668en_HK
dc.identifier.issn0952-5238en_HK
dc.identifier.urihttp://hdl.handle.net/10722/42335-
dc.description.abstractAxonal regeneration of retinal ganglion cells (RGCs) into a normal or pre-degenerated peripheral nerve graft after an optic nerve pre-lesion was investigated. A pre-lesion performed 1-2 weeks before a second lesion has been shown to enhance axonal regeneration in peripheral nerves (PN) but not in optic nerves (ON) in mammals. The lack of such a beneficial pre-lesion effect may be due to the long delay (1-6 weeks) between the two lesions since RGCs and their axons degenerate rapidly 1-2 weeks following axotomy in adult rodents. The present study examined the effects of the proximal and distal ON pre-lesions with a shortened delay (0-8 days) on axonal regeneration of RGCs through a normal or pre-degenerated PN graft. The ON of adult hamsters was transected intraorbitally at 2 mm (proximal lesion) or intracranially at 7 mm (distal lesion) from the optic disc. The pre-lesioned ON was re-transected at 0.5 mm from the disc after 0, 1, 2, 4, or 8 days and a normal or a pre-degenerated PN graft was attached onto the ocular stump. The number of RGCs regenerating their injured axons into the PN graft was estimated by retrograde labeling with FluoroGold 4 weeks after grafting. The number of regenerating RGCs decreased significantly when the delay-time increased in animals with both the ON pre-lesions (proximal or distal) compared to control animals without an ON pre-lesion. The proximal ON pre-lesion significantly reduced the number of regenerating RGCs after a delay of 8 days in comparison with the distal lesion. However, this adverse effect can be overcome, to some degree, by a pre-degenerated PN graft applied 2, 4, or 8 days after the distal ON pre-lesion enhanced more RGCs to regenerate than the normal PN graft. Thus, in order to obtain the highest number of regenerating RGCs, a pre-degenerated PN should be grafted immediately after an ON lesion.en_HK
dc.format.extent334315 bytes-
dc.format.extent25088 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.languageengen_HK
dc.publisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=VNSen_HK
dc.relation.ispartofVisual Neuroscienceen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsVisual Neuroscience. Copyright © Cambridge University Press.en_HK
dc.subjectOptic nerveen_HK
dc.subjectPre-lesionen_HK
dc.subjectRegenerationen_HK
dc.subjectRetinal ganglion cellen_HK
dc.subjectTransplantationen_HK
dc.subject.meshAxons - physiologyen_HK
dc.subject.meshNerve degeneration - physiopathologyen_HK
dc.subject.meshNerve regeneration - physiologyen_HK
dc.subject.meshOptic nerve injuries - physiopathologyen_HK
dc.subject.meshPeripheral nerves - physiopathology - transplantationen_HK
dc.titleAxonal regeneration of retinal ganglion cells after optic nerve pre-lesions and attachment of normal or pre-degenerated peripheral nerve graftsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0952-5238&volume=19&issue=5&spage=661&epage=668&date=2002&atitle=Axonal+regeneration+of+retinal+ganglion+cells+after+optic+nerve+pre-lesions+and+attachment+of+normal+or+pre-degenerated+peripheral+nerve+graftsen_HK
dc.identifier.emailYip, HK:hkfyip@hku.hken_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.authorityYip, HK=rp00285en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1017/S0952523802195113en_HK
dc.identifier.pmid12507332-
dc.identifier.scopuseid_2-s2.0-0036763862en_HK
dc.identifier.hkuros74971-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036763862&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume19en_HK
dc.identifier.issue5en_HK
dc.identifier.spage661en_HK
dc.identifier.epage668en_HK
dc.identifier.isiWOS:000179829900011-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYou, SW=8226423300en_HK
dc.identifier.scopusauthoridBedi, KS=7005465634en_HK
dc.identifier.scopusauthoridYip, HK=7101980864en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK

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