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Article: Axonal regeneration of retinal ganglion cells after optic nerve pre-lesions and attachment of normal or pre-degenerated peripheral nerve grafts
Title | Axonal regeneration of retinal ganglion cells after optic nerve pre-lesions and attachment of normal or pre-degenerated peripheral nerve grafts |
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Authors | |
Keywords | Optic nerve Pre-lesion Regeneration Retinal ganglion cell Transplantation |
Issue Date | 2002 |
Publisher | Cambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=VNS |
Citation | Visual Neuroscience, 2002, v. 19 n. 5, p. 661-668 How to Cite? |
Abstract | Axonal regeneration of retinal ganglion cells (RGCs) into a normal or pre-degenerated peripheral nerve graft after an optic nerve pre-lesion was investigated. A pre-lesion performed 1-2 weeks before a second lesion has been shown to enhance axonal regeneration in peripheral nerves (PN) but not in optic nerves (ON) in mammals. The lack of such a beneficial pre-lesion effect may be due to the long delay (1-6 weeks) between the two lesions since RGCs and their axons degenerate rapidly 1-2 weeks following axotomy in adult rodents. The present study examined the effects of the proximal and distal ON pre-lesions with a shortened delay (0-8 days) on axonal regeneration of RGCs through a normal or pre-degenerated PN graft. The ON of adult hamsters was transected intraorbitally at 2 mm (proximal lesion) or intracranially at 7 mm (distal lesion) from the optic disc. The pre-lesioned ON was re-transected at 0.5 mm from the disc after 0, 1, 2, 4, or 8 days and a normal or a pre-degenerated PN graft was attached onto the ocular stump. The number of RGCs regenerating their injured axons into the PN graft was estimated by retrograde labeling with FluoroGold 4 weeks after grafting. The number of regenerating RGCs decreased significantly when the delay-time increased in animals with both the ON pre-lesions (proximal or distal) compared to control animals without an ON pre-lesion. The proximal ON pre-lesion significantly reduced the number of regenerating RGCs after a delay of 8 days in comparison with the distal lesion. However, this adverse effect can be overcome, to some degree, by a pre-degenerated PN graft applied 2, 4, or 8 days after the distal ON pre-lesion enhanced more RGCs to regenerate than the normal PN graft. Thus, in order to obtain the highest number of regenerating RGCs, a pre-degenerated PN should be grafted immediately after an ON lesion. |
Persistent Identifier | http://hdl.handle.net/10722/42335 |
ISSN | 2023 Impact Factor: 1.1 2023 SCImago Journal Rankings: 0.472 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | You, SW | en_HK |
dc.contributor.author | Bedi, KS | en_HK |
dc.contributor.author | Yip, HK | en_HK |
dc.contributor.author | So, KF | en_HK |
dc.date.accessioned | 2007-01-29T08:47:09Z | - |
dc.date.available | 2007-01-29T08:47:09Z | - |
dc.date.issued | 2002 | en_HK |
dc.identifier.citation | Visual Neuroscience, 2002, v. 19 n. 5, p. 661-668 | en_HK |
dc.identifier.issn | 0952-5238 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/42335 | - |
dc.description.abstract | Axonal regeneration of retinal ganglion cells (RGCs) into a normal or pre-degenerated peripheral nerve graft after an optic nerve pre-lesion was investigated. A pre-lesion performed 1-2 weeks before a second lesion has been shown to enhance axonal regeneration in peripheral nerves (PN) but not in optic nerves (ON) in mammals. The lack of such a beneficial pre-lesion effect may be due to the long delay (1-6 weeks) between the two lesions since RGCs and their axons degenerate rapidly 1-2 weeks following axotomy in adult rodents. The present study examined the effects of the proximal and distal ON pre-lesions with a shortened delay (0-8 days) on axonal regeneration of RGCs through a normal or pre-degenerated PN graft. The ON of adult hamsters was transected intraorbitally at 2 mm (proximal lesion) or intracranially at 7 mm (distal lesion) from the optic disc. The pre-lesioned ON was re-transected at 0.5 mm from the disc after 0, 1, 2, 4, or 8 days and a normal or a pre-degenerated PN graft was attached onto the ocular stump. The number of RGCs regenerating their injured axons into the PN graft was estimated by retrograde labeling with FluoroGold 4 weeks after grafting. The number of regenerating RGCs decreased significantly when the delay-time increased in animals with both the ON pre-lesions (proximal or distal) compared to control animals without an ON pre-lesion. The proximal ON pre-lesion significantly reduced the number of regenerating RGCs after a delay of 8 days in comparison with the distal lesion. However, this adverse effect can be overcome, to some degree, by a pre-degenerated PN graft applied 2, 4, or 8 days after the distal ON pre-lesion enhanced more RGCs to regenerate than the normal PN graft. Thus, in order to obtain the highest number of regenerating RGCs, a pre-degenerated PN should be grafted immediately after an ON lesion. | en_HK |
dc.format.extent | 334315 bytes | - |
dc.format.extent | 25088 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.format.mimetype | application/msword | - |
dc.language | eng | en_HK |
dc.publisher | Cambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=VNS | en_HK |
dc.relation.ispartof | Visual Neuroscience | en_HK |
dc.rights | Visual Neuroscience. Copyright © Cambridge University Press. | en_HK |
dc.subject | Optic nerve | en_HK |
dc.subject | Pre-lesion | en_HK |
dc.subject | Regeneration | en_HK |
dc.subject | Retinal ganglion cell | en_HK |
dc.subject | Transplantation | en_HK |
dc.subject.mesh | Axons - physiology | en_HK |
dc.subject.mesh | Nerve degeneration - physiopathology | en_HK |
dc.subject.mesh | Nerve regeneration - physiology | en_HK |
dc.subject.mesh | Optic nerve injuries - physiopathology | en_HK |
dc.subject.mesh | Peripheral nerves - physiopathology - transplantation | en_HK |
dc.title | Axonal regeneration of retinal ganglion cells after optic nerve pre-lesions and attachment of normal or pre-degenerated peripheral nerve grafts | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0952-5238&volume=19&issue=5&spage=661&epage=668&date=2002&atitle=Axonal+regeneration+of+retinal+ganglion+cells+after+optic+nerve+pre-lesions+and+attachment+of+normal+or+pre-degenerated+peripheral+nerve+grafts | en_HK |
dc.identifier.email | Yip, HK:hkfyip@hku.hk | en_HK |
dc.identifier.email | So, KF:hrmaskf@hkucc.hku.hk | en_HK |
dc.identifier.authority | Yip, HK=rp00285 | en_HK |
dc.identifier.authority | So, KF=rp00329 | en_HK |
dc.description.nature | published_or_final_version | en_HK |
dc.identifier.doi | 10.1017/S0952523802195113 | en_HK |
dc.identifier.pmid | 12507332 | - |
dc.identifier.scopus | eid_2-s2.0-0036763862 | en_HK |
dc.identifier.hkuros | 74971 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0036763862&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 19 | en_HK |
dc.identifier.issue | 5 | en_HK |
dc.identifier.spage | 661 | en_HK |
dc.identifier.epage | 668 | en_HK |
dc.identifier.isi | WOS:000179829900011 | - |
dc.publisher.place | United Kingdom | en_HK |
dc.identifier.scopusauthorid | You, SW=8226423300 | en_HK |
dc.identifier.scopusauthorid | Bedi, KS=7005465634 | en_HK |
dc.identifier.scopusauthorid | Yip, HK=7101980864 | en_HK |
dc.identifier.scopusauthorid | So, KF=34668391300 | en_HK |
dc.identifier.issnl | 0952-5238 | - |