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Article: Substance P-immunoreactive neurons in hamster retinas

TitleSubstance P-immunoreactive neurons in hamster retinas
Authors
KeywordsAmacrine cells
Dorsal lateral geniculate nucleus
Optic nerve section
Retinal ganglion cells
Substance P
Issue Date1999
PublisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=VNS
Citation
Visual Neuroscience, 1999, v. 16 n. 3, p. 475-481 How to Cite?
AbstractLight-microscopic immunocytochemistry was utilized to localize the different populations of substance P-immunoreactive (SP-IR) neurons in the hamster retina. Based on observation of 2505 SP-IR neurons in transverse sections, 34% were amacrine cells whose pear-shaped or round cell bodies (7-8 μm) were situated in the inner half of the inner nuclear layer (INL) or in the inner plexiform layer (IPL), while 66% of SP-IR somata (6-20 μm) were located in the ganglion cell layer (GCL) which were interpreted to be displaced amacrine cells and retinal ganglion cells (RGCs). At least three types of SP-IR amacrine cells were identified. The SP-IR processes were distributed in strata 1, 3, and 5 with the densest plexus in stratum 5 of the inner plexiform layer. In the wholemounted retina, the SP-IR cells were found to be distributed throughout the entire retina and their mean number was estimated to be 4224 ± 76. Two experiments were performed to clarify whether any of the SP-IR neurons in the GCL were RGCs. The first experiment demonstrated the presence of SP-IR RGCs by retrogradely labeling the RGCs and subsequently staining the SP-IR cells in the retina using immunocytochemistry. The second experiment identified SP-IR central projections of RGCs to the contralateral dorsal lateral geniculate nucleus. This projection disappeared following removal of the contralateral eye. The number of SP-IR RGCs was estimated following optic nerve section. At 2 months after sectioning the optic nerve, the total number of SP-IR neurons in the GCL reduced from 4224 ± 76 to a mean of 1192 ± 139. Assuming that all SP-IR neurons in the GCL which disappeared after nerve section were RGCs, the number of SP-IR RGCs was estimated to be 3032, representing 3-4% of the total RGCs. In summary, findings of the present study provide evidence for the existence of SP-IR RGCs in the hamster retina.
Persistent Identifierhttp://hdl.handle.net/10722/42334
ISSN
2015 Impact Factor: 1.871
2015 SCImago Journal Rankings: 1.231
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, HBen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorCheuk, Wen_HK
dc.date.accessioned2007-01-29T08:47:08Z-
dc.date.available2007-01-29T08:47:08Z-
dc.date.issued1999en_HK
dc.identifier.citationVisual Neuroscience, 1999, v. 16 n. 3, p. 475-481en_HK
dc.identifier.issn0952-5238en_HK
dc.identifier.urihttp://hdl.handle.net/10722/42334-
dc.description.abstractLight-microscopic immunocytochemistry was utilized to localize the different populations of substance P-immunoreactive (SP-IR) neurons in the hamster retina. Based on observation of 2505 SP-IR neurons in transverse sections, 34% were amacrine cells whose pear-shaped or round cell bodies (7-8 μm) were situated in the inner half of the inner nuclear layer (INL) or in the inner plexiform layer (IPL), while 66% of SP-IR somata (6-20 μm) were located in the ganglion cell layer (GCL) which were interpreted to be displaced amacrine cells and retinal ganglion cells (RGCs). At least three types of SP-IR amacrine cells were identified. The SP-IR processes were distributed in strata 1, 3, and 5 with the densest plexus in stratum 5 of the inner plexiform layer. In the wholemounted retina, the SP-IR cells were found to be distributed throughout the entire retina and their mean number was estimated to be 4224 ± 76. Two experiments were performed to clarify whether any of the SP-IR neurons in the GCL were RGCs. The first experiment demonstrated the presence of SP-IR RGCs by retrogradely labeling the RGCs and subsequently staining the SP-IR cells in the retina using immunocytochemistry. The second experiment identified SP-IR central projections of RGCs to the contralateral dorsal lateral geniculate nucleus. This projection disappeared following removal of the contralateral eye. The number of SP-IR RGCs was estimated following optic nerve section. At 2 months after sectioning the optic nerve, the total number of SP-IR neurons in the GCL reduced from 4224 ± 76 to a mean of 1192 ± 139. Assuming that all SP-IR neurons in the GCL which disappeared after nerve section were RGCs, the number of SP-IR RGCs was estimated to be 3032, representing 3-4% of the total RGCs. In summary, findings of the present study provide evidence for the existence of SP-IR RGCs in the hamster retina.en_HK
dc.format.extent978680 bytes-
dc.format.extent25600 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.languageengen_HK
dc.publisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=VNSen_HK
dc.relation.ispartofVisual Neuroscienceen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsVisual Neuroscience. Copyright © Cambridge University Press.en_HK
dc.subjectAmacrine cellsen_HK
dc.subjectDorsal lateral geniculate nucleusen_HK
dc.subjectOptic nerve sectionen_HK
dc.subjectRetinal ganglion cellsen_HK
dc.subjectSubstance Pen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshNeurons - chemistryen_HK
dc.subject.meshRetina - chemistry - cytologyen_HK
dc.subject.meshRetinal ganglion cells - chemistryen_HK
dc.subject.meshSubstance p - analysisen_HK
dc.titleSubstance P-immunoreactive neurons in hamster retinasen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0952-5238&volume=16&issue=3&spage=475&epage=481&date=1999&atitle=Substance+P-immunoreactive+neurons+in+hamster+retinasen_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1017/S0952523899163089en_HK
dc.identifier.pmid10349968-
dc.identifier.scopuseid_2-s2.0-0033136353en_HK
dc.identifier.hkuros40883-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033136353&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume16en_HK
dc.identifier.issue3en_HK
dc.identifier.spage475en_HK
dc.identifier.epage481en_HK
dc.identifier.isiWOS:000080234000008-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLi, HB=13008179800en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK
dc.identifier.scopusauthoridCheuk, W=7003958433en_HK

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