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Article: Generation and chromosome mapping of expressed sequence tags (ESTs) from a human infant thymus

TitleGeneration and chromosome mapping of expressed sequence tags (ESTs) from a human infant thymus
Authors
KeywordsCDNA
Disease locus
Expression profile
Radiation hybrid mapping
Issue Date1999
PublisherN R C Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?gen
Citation
Genome, 1999, v. 42 n. 3, p. 457-464 How to Cite?
AbstractIn an effort to identify novel genes that are expressed differentially in an infant thymus, we constructed an oligo-d(T) primed cDNA library from a human infant thymus followed by single-run partial sequencing to generate expressed sequence tags (ESTs). Characterization of more than 1400 sequences enabled us to convert human thymus transcripts into 1223 useful ESTs. These ESTs consisted of 613 (50.1%) showing homology to known human genes, 51 (4.2%) matching to genes from other species, 289 (23.6%) matching ESTs of unknown functions, and 182 (14.9%) being novel transcripts. The expression profile of an infant thymus features a high number of genes related to cell division-DNA synthesis and gene-protein expression, indicating the active growth stage of an infant thymus. To identify the chromosomal localization of 43 thymus ESTs, PCR-based mapping was performed using a human-rodent somatic cell hybrid or radiation hybrid mapping panel. The results indicated that several novel genes were determined to be located in the vicinity of previously mapped disease loci; histidinemia loci, plasminogen Tochigi disease loci, Ehlers-Danlos syndrome, hypertriglyceridemia, thyroid resistance locus, ocular albinism, galactosemia, porphyria variegata, Charcot-Marie-tooth disease, FEOM (fibrosis of extraocular muscles), Prader-Willi syndrome.
Persistent Identifierhttp://hdl.handle.net/10722/42308
ISSN
2015 Impact Factor: 1.356
2015 SCImago Journal Rankings: 0.666
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHwang, MYen_HK
dc.contributor.authorKang, YJen_HK
dc.contributor.authorKim, YHen_HK
dc.contributor.authorScherer, SWen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorSohn, Uen_HK
dc.date.accessioned2007-01-08T02:34:12Z-
dc.date.available2007-01-08T02:34:12Z-
dc.date.issued1999en_HK
dc.identifier.citationGenome, 1999, v. 42 n. 3, p. 457-464en_HK
dc.identifier.issn0831-2796en_HK
dc.identifier.urihttp://hdl.handle.net/10722/42308-
dc.description.abstractIn an effort to identify novel genes that are expressed differentially in an infant thymus, we constructed an oligo-d(T) primed cDNA library from a human infant thymus followed by single-run partial sequencing to generate expressed sequence tags (ESTs). Characterization of more than 1400 sequences enabled us to convert human thymus transcripts into 1223 useful ESTs. These ESTs consisted of 613 (50.1%) showing homology to known human genes, 51 (4.2%) matching to genes from other species, 289 (23.6%) matching ESTs of unknown functions, and 182 (14.9%) being novel transcripts. The expression profile of an infant thymus features a high number of genes related to cell division-DNA synthesis and gene-protein expression, indicating the active growth stage of an infant thymus. To identify the chromosomal localization of 43 thymus ESTs, PCR-based mapping was performed using a human-rodent somatic cell hybrid or radiation hybrid mapping panel. The results indicated that several novel genes were determined to be located in the vicinity of previously mapped disease loci; histidinemia loci, plasminogen Tochigi disease loci, Ehlers-Danlos syndrome, hypertriglyceridemia, thyroid resistance locus, ocular albinism, galactosemia, porphyria variegata, Charcot-Marie-tooth disease, FEOM (fibrosis of extraocular muscles), Prader-Willi syndrome.en_HK
dc.format.extent1234247 bytes-
dc.format.extent30208 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.languageengen_HK
dc.publisherN R C Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?genen_HK
dc.relation.ispartofGenomeen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsGenome. Copyright © N R C Research Press.en_HK
dc.subjectCDNAen_HK
dc.subjectDisease locusen_HK
dc.subjectExpression profileen_HK
dc.subjectRadiation hybrid mappingen_HK
dc.subject.meshChromosome mappingen_HK
dc.subject.meshExpressed sequence tagsen_HK
dc.subject.meshThymus gland - metabolismen_HK
dc.subject.meshProteins - geneticsen_HK
dc.subject.meshEnzymes - geneticsen_HK
dc.titleGeneration and chromosome mapping of expressed sequence tags (ESTs) from a human infant thymusen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0831-2796&volume=42&issue=3&spage=457&epage=464&date=1999&atitle=Generation+and+chromosome+mapping+of+expressed+sequence+tags+(ESTs)+from+a+human+infant+thymusen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid10382293-
dc.identifier.scopuseid_2-s2.0-0033150469en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033150469&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume42en_HK
dc.identifier.issue3en_HK
dc.identifier.spage457en_HK
dc.identifier.epage464en_HK
dc.identifier.isiWOS:000080857700012-
dc.publisher.placeCanadaen_HK
dc.identifier.scopusauthoridHwang, MY=36872259100en_HK
dc.identifier.scopusauthoridKang, YJ=7402784367en_HK
dc.identifier.scopusauthoridKim, YH=34770762300en_HK
dc.identifier.scopusauthoridScherer, SW=55159183300en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridSohn, U=7005966409en_HK

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