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Article: Permeability of wild-type and mutant cystic fibrosis transmembrane conductance regulator chloride channels to polyatomic anions

TitlePermeability of wild-type and mutant cystic fibrosis transmembrane conductance regulator chloride channels to polyatomic anions
Authors
KeywordsAnion permeation
Channel selectivity
Cystic fibrosis
Lyotropic sequence
Pore size
Issue Date1997
PublisherRockefeller University Press. The Journal's web site is located at www.jgp.org/
Citation
Journal Of General Physiology, 1997, v. 110 n. 4, p. 355-364 How to Cite?
AbstractPermeability of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel to polyatomic anions of known dimensions was studied in stably transfected Chinese hamster ovary cells by using the patch clamp technique. Biionic reversal potentials measured with external polyatomic anions gave the permeability ratio (P(X)/P(Cl)) sequence NO3/- > Cl- > HCO3/- > formate > acetate. The same selectivity sequence but somewhat higher permeability ratios were obtained when anions were tested from the cytoplasmic side. Pyruvate, propanoate, methane sulfonate, ethane sulfonate, and gluconate were not measurably permeant (P(X)/P(Cl) < 0.06) from either side of the membrane. The relationship between permeability ratios from the outside and ionic diameters suggests a minimum functional pore diameter of ~5.3 Å. Permeability ratios also followed a lyotropic sequence, suggesting that permeability is dependent on ionic hydration energies. Site-directed mutagenesis of two adjacent threonines in TM6 to smaller, less polar alanines led to a significant (24%) increase in single channel conductance and elevated permeability to several large anions, suggesting that these residues do not strongly bind permeating artions, but may contribute to the narrowest part of the pore.
Persistent Identifierhttp://hdl.handle.net/10722/42306
ISSN
2015 Impact Factor: 4.511
2015 SCImago Journal Rankings: 3.061
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLinsdell, Pen_HK
dc.contributor.authorTabcharani, JAen_HK
dc.contributor.authorRommens, JMen_HK
dc.contributor.authorHou, YXen_HK
dc.contributor.authorChang, XBen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorRiordan, JRen_HK
dc.contributor.authorHanrahan, JWen_HK
dc.date.accessioned2007-01-08T02:34:10Z-
dc.date.available2007-01-08T02:34:10Z-
dc.date.issued1997en_HK
dc.identifier.citationJournal Of General Physiology, 1997, v. 110 n. 4, p. 355-364en_HK
dc.identifier.issn0022-1295en_HK
dc.identifier.urihttp://hdl.handle.net/10722/42306-
dc.description.abstractPermeability of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel to polyatomic anions of known dimensions was studied in stably transfected Chinese hamster ovary cells by using the patch clamp technique. Biionic reversal potentials measured with external polyatomic anions gave the permeability ratio (P(X)/P(Cl)) sequence NO3/- > Cl- > HCO3/- > formate > acetate. The same selectivity sequence but somewhat higher permeability ratios were obtained when anions were tested from the cytoplasmic side. Pyruvate, propanoate, methane sulfonate, ethane sulfonate, and gluconate were not measurably permeant (P(X)/P(Cl) < 0.06) from either side of the membrane. The relationship between permeability ratios from the outside and ionic diameters suggests a minimum functional pore diameter of ~5.3 Å. Permeability ratios also followed a lyotropic sequence, suggesting that permeability is dependent on ionic hydration energies. Site-directed mutagenesis of two adjacent threonines in TM6 to smaller, less polar alanines led to a significant (24%) increase in single channel conductance and elevated permeability to several large anions, suggesting that these residues do not strongly bind permeating artions, but may contribute to the narrowest part of the pore.en_HK
dc.format.extent235272 bytes-
dc.format.extent30208 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.languageengen_HK
dc.publisherRockefeller University Press. The Journal's web site is located at www.jgp.org/en_HK
dc.relation.ispartofJournal of General Physiologyen_HK
dc.rightsJournal of general physiology. Copyright © Rockefeller University Press.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectAnion permeationen_HK
dc.subjectChannel selectivityen_HK
dc.subjectCystic fibrosisen_HK
dc.subjectLyotropic sequenceen_HK
dc.subjectPore sizeen_HK
dc.subject.meshAnions - metabolismen_HK
dc.subject.meshCystic fibrosis transmembrane conductance regulator - genetics - metabolismen_HK
dc.subject.meshGene expression regulationen_HK
dc.subject.meshIon channel gating - genetics - physiologyen_HK
dc.subject.meshMembrane potentials - physiologyen_HK
dc.titlePermeability of wild-type and mutant cystic fibrosis transmembrane conductance regulator chloride channels to polyatomic anionsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-1295&volume=110&issue=4&spage=355&epage=364&date=1997&atitle=Permeability+of+Wild-Type+and+Mutant+Cystic+Fibrosis+Transmembrane+Conductance+Regulator+Chloride+Channels+to+Polyatomic+Anionsen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1085/jgp.110.4.355en_HK
dc.identifier.pmid9379168-
dc.identifier.pmcidPMC2229373-
dc.identifier.scopuseid_2-s2.0-0030885564en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030885564&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume110en_HK
dc.identifier.issue4en_HK
dc.identifier.spage355en_HK
dc.identifier.epage364en_HK
dc.identifier.isiWOS:A1997YA24400003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLinsdell, P=7004694661en_HK
dc.identifier.scopusauthoridTabcharani, JA=6701582734en_HK
dc.identifier.scopusauthoridRommens, JM=7006884140en_HK
dc.identifier.scopusauthoridHou, YX=7402198832en_HK
dc.identifier.scopusauthoridChang, XB=7202486073en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridRiordan, JR=7202229758en_HK
dc.identifier.scopusauthoridHanrahan, JW=7103386837en_HK

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