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Article: Physical localization of two DNA markers closely linked to the cystic fibrosis locus by pulsed-field gel electrophoresis

TitlePhysical localization of two DNA markers closely linked to the cystic fibrosis locus by pulsed-field gel electrophoresis
Authors
Issue Date1989
PublisherCell Press. The Journal's web site is located at http://www.cell.com/AJHG/
Citation
American Journal Of Human Genetics, 1989, v. 45 n. 6, p. 932-941 How to Cite?
AbstractOur previous linkage analysis suggested that the DNA segment D7S122 is located between MET and D7S8, the two genetic markers that are thought to flank the cystic fibrosis locus (CF). Subsequent chromosome walking experiments revealed that D7S122 is within close distance to another randomly isolated DNA marker, D7S340. To determine the physical relationship among D7S122, D7S340, MET, and D7S8, we have constructed a long-range restriction map of the region containing these four DNA segments, by using DNA from a human/hamster somatic hybrid cell line 4AF-KO15 (containing a single human chromosome 7) and a series of rare-cutting restriction enzymes. The combined results of complete, partial, and double digestion analyses confirm that D7S122 and D7S340 are located between MET and D7S8. The order of these markers is MET-D7S340-D7S122-D7S8, with distance intervals of approximately 500, 10, and 980 kbp, respectively. Together with family analysis, this information will be useful for eventual identification of the CF gene.
Persistent Identifierhttp://hdl.handle.net/10722/42291
ISSN
2015 Impact Factor: 10.794
2015 SCImago Journal Rankings: 8.769
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorRommens, JMen_HK
dc.contributor.authorZengerlingLentes, Sen_HK
dc.contributor.authorKerem, BSen_HK
dc.contributor.authorMelmer, Gen_HK
dc.contributor.authorBuchwald, Men_HK
dc.contributor.authorTsui, LCen_HK
dc.date.accessioned2007-01-08T02:33:50Z-
dc.date.available2007-01-08T02:33:50Z-
dc.date.issued1989en_HK
dc.identifier.citationAmerican Journal Of Human Genetics, 1989, v. 45 n. 6, p. 932-941en_HK
dc.identifier.issn0002-9297en_HK
dc.identifier.urihttp://hdl.handle.net/10722/42291-
dc.description.abstractOur previous linkage analysis suggested that the DNA segment D7S122 is located between MET and D7S8, the two genetic markers that are thought to flank the cystic fibrosis locus (CF). Subsequent chromosome walking experiments revealed that D7S122 is within close distance to another randomly isolated DNA marker, D7S340. To determine the physical relationship among D7S122, D7S340, MET, and D7S8, we have constructed a long-range restriction map of the region containing these four DNA segments, by using DNA from a human/hamster somatic hybrid cell line 4AF-KO15 (containing a single human chromosome 7) and a series of rare-cutting restriction enzymes. The combined results of complete, partial, and double digestion analyses confirm that D7S122 and D7S340 are located between MET and D7S8. The order of these markers is MET-D7S340-D7S122-D7S8, with distance intervals of approximately 500, 10, and 980 kbp, respectively. Together with family analysis, this information will be useful for eventual identification of the CF gene.en_HK
dc.format.extent3738667 bytes-
dc.format.extent30208 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.languageengen_HK
dc.publisherCell Press. The Journal's web site is located at http://www.cell.com/AJHG/en_HK
dc.relation.ispartofAmerican Journal of Human Geneticsen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsAmerican journal of human genetics. Copyright © University of Chicago Press.en_HK
dc.subject.meshCystic fibrosis - geneticsen_HK
dc.subject.meshElectrophoresis, agar gelen_HK
dc.subject.meshGenetic markersen_HK
dc.subject.meshLinkage (genetics)en_HK
dc.subject.meshRestriction mappingen_HK
dc.titlePhysical localization of two DNA markers closely linked to the cystic fibrosis locus by pulsed-field gel electrophoresisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0002-9297&volume=45&issue=6&spage=932&epage=941&date=1989&atitle=Physical+localization+of+two+DNA+markers+closely+linked+to+the+cystic+fibrosis+by+pulsed+field+gel+electrophoresisen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid2589321-
dc.identifier.pmcidPMC1683484-
dc.identifier.scopuseid_2-s2.0-0024784387en_HK
dc.identifier.volume45en_HK
dc.identifier.issue6en_HK
dc.identifier.spage932en_HK
dc.identifier.epage941en_HK
dc.identifier.isiWOS:A1989CF40300013-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridRommens, JM=7006884140en_HK
dc.identifier.scopusauthoridZengerlingLentes, S=6504746268en_HK
dc.identifier.scopusauthoridKerem, BS=35376353800en_HK
dc.identifier.scopusauthoridMelmer, G=7003309613en_HK
dc.identifier.scopusauthoridBuchwald, M=7006759922en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK

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