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Article: Identification and regional localization of DNA markers on chromosome 7 for the cloning of the cystic fibrosis gene
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TitleIdentification and regional localization of DNA markers on chromosome 7 for the cloning of the cystic fibrosis gene
 
AuthorsRommens, JM1
Zengerling, S1
Burns, J1
Melmer, G1
Kerem, B1
Plavsic, N1
Zsiga, M1
Kennedy, D1
Markiewicz, D1
Rozmahel, R1
Riordan, JR1
Buchwald, M1
Tsui, L1
 
Issue Date1988
 
PublisherCell Press. The Journal's web site is located at http://www.cell.com/AJHG/
 
CitationAmerican Journal Of Human Genetics, 1988, v. 43 n. 5, p. 645-663 [How to Cite?]
 
AbstractTo facilitate mapping of the cystic fibrosis locus (CF) and to isolate the corresponding gene, we have screened a flow-sorted chromosome 7-specific library for additional DNA markers in the 7q31-q32 region. Unique ('single-copy') DNA segments were selected from the library and used in hybridization analysis with a panel of somatic cell hybrids containing various portions of human chromosome 7 and patient cell lines with deletion of this chromosome. A total of 258 chromosome 7-specific single-copy DNA segments were identified, and most of them localized to subregions. Fifty three of these corresponded to DNA sequences in the 7q31-q32 region. Family and physical mapping studies showed that two of the DNA markers, D7S122 and D7S340, are in close linkage with CF. The data also showed that D7S122 and D7S340 map between MET and D7S8, the two genetic markers known to be on opposite sides of CF. The study thus reaffirms the general strategy in approaching a disease locus on the basis of chromosome location.
 
ISSN0002-9297
2013 Impact Factor: 10.987
 
PubMed Central IDPMC1715526
 
ISI Accession Number IDWOS:A1988Q933600011
 
DC FieldValue
dc.contributor.authorRommens, JM
 
dc.contributor.authorZengerling, S
 
dc.contributor.authorBurns, J
 
dc.contributor.authorMelmer, G
 
dc.contributor.authorKerem, B
 
dc.contributor.authorPlavsic, N
 
dc.contributor.authorZsiga, M
 
dc.contributor.authorKennedy, D
 
dc.contributor.authorMarkiewicz, D
 
dc.contributor.authorRozmahel, R
 
dc.contributor.authorRiordan, JR
 
dc.contributor.authorBuchwald, M
 
dc.contributor.authorTsui, L
 
dc.date.accessioned2007-01-08T02:33:47Z
 
dc.date.available2007-01-08T02:33:47Z
 
dc.date.issued1988
 
dc.description.abstractTo facilitate mapping of the cystic fibrosis locus (CF) and to isolate the corresponding gene, we have screened a flow-sorted chromosome 7-specific library for additional DNA markers in the 7q31-q32 region. Unique ('single-copy') DNA segments were selected from the library and used in hybridization analysis with a panel of somatic cell hybrids containing various portions of human chromosome 7 and patient cell lines with deletion of this chromosome. A total of 258 chromosome 7-specific single-copy DNA segments were identified, and most of them localized to subregions. Fifty three of these corresponded to DNA sequences in the 7q31-q32 region. Family and physical mapping studies showed that two of the DNA markers, D7S122 and D7S340, are in close linkage with CF. The data also showed that D7S122 and D7S340 map between MET and D7S8, the two genetic markers known to be on opposite sides of CF. The study thus reaffirms the general strategy in approaching a disease locus on the basis of chromosome location.
 
dc.description.naturepublished_or_final_version
 
dc.format.extent3656109 bytes
 
dc.format.extent30208 bytes
 
dc.format.mimetypeapplication/pdf
 
dc.format.mimetypeapplication/msword
 
dc.identifier.citationAmerican Journal Of Human Genetics, 1988, v. 43 n. 5, p. 645-663 [How to Cite?]
 
dc.identifier.epage663
 
dc.identifier.isiWOS:A1988Q933600011
 
dc.identifier.issn0002-9297
2013 Impact Factor: 10.987
 
dc.identifier.issue5
 
dc.identifier.openurl
 
dc.identifier.pmcidPMC1715526
 
dc.identifier.pmid2903665
 
dc.identifier.scopuseid_2-s2.0-0023700081
 
dc.identifier.spage645
 
dc.identifier.urihttp://hdl.handle.net/10722/42289
 
dc.identifier.volume43
 
dc.languageeng
 
dc.publisherCell Press. The Journal's web site is located at http://www.cell.com/AJHG/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofAmerican Journal of Human Genetics
 
dc.rightsAmerican journal of human genetics. Copyright © University of Chicago Press.
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshChromosome banding
 
dc.subject.meshChromosomes, human, pair 7
 
dc.subject.meshCloning, molecular
 
dc.subject.meshCystic fibrosis - genetics
 
dc.subject.meshDna probes
 
dc.titleIdentification and regional localization of DNA markers on chromosome 7 for the cloning of the cystic fibrosis gene
 
dc.typeArticle
 
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<contributor.author>Kennedy, D</contributor.author>
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<contributor.author>Riordan, JR</contributor.author>
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<contributor.author>Tsui, L</contributor.author>
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Author Affiliations
  1. Hospital for Sick Children University of Toronto