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Article: Identification and regional localization of DNA markers on chromosome 7 for the cloning of the cystic fibrosis gene

TitleIdentification and regional localization of DNA markers on chromosome 7 for the cloning of the cystic fibrosis gene
Authors
Issue Date1988
PublisherCell Press. The Journal's web site is located at http://www.cell.com/AJHG/
Citation
American Journal Of Human Genetics, 1988, v. 43 n. 5, p. 645-663 How to Cite?
Abstract
To facilitate mapping of the cystic fibrosis locus (CF) and to isolate the corresponding gene, we have screened a flow-sorted chromosome 7-specific library for additional DNA markers in the 7q31-q32 region. Unique ('single-copy') DNA segments were selected from the library and used in hybridization analysis with a panel of somatic cell hybrids containing various portions of human chromosome 7 and patient cell lines with deletion of this chromosome. A total of 258 chromosome 7-specific single-copy DNA segments were identified, and most of them localized to subregions. Fifty three of these corresponded to DNA sequences in the 7q31-q32 region. Family and physical mapping studies showed that two of the DNA markers, D7S122 and D7S340, are in close linkage with CF. The data also showed that D7S122 and D7S340 map between MET and D7S8, the two genetic markers known to be on opposite sides of CF. The study thus reaffirms the general strategy in approaching a disease locus on the basis of chromosome location.
Persistent Identifierhttp://hdl.handle.net/10722/42289
ISSN
2013 Impact Factor: 10.987
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorRommens, JMen_HK
dc.contributor.authorZengerling, Sen_HK
dc.contributor.authorBurns, Jen_HK
dc.contributor.authorMelmer, Gen_HK
dc.contributor.authorKerem, Ben_HK
dc.contributor.authorPlavsic, Nen_HK
dc.contributor.authorZsiga, Men_HK
dc.contributor.authorKennedy, Den_HK
dc.contributor.authorMarkiewicz, Den_HK
dc.contributor.authorRozmahel, Ren_HK
dc.contributor.authorRiordan, JRen_HK
dc.contributor.authorBuchwald, Men_HK
dc.contributor.authorTsui, Len_HK
dc.date.accessioned2007-01-08T02:33:47Z-
dc.date.available2007-01-08T02:33:47Z-
dc.date.issued1988en_HK
dc.identifier.citationAmerican Journal Of Human Genetics, 1988, v. 43 n. 5, p. 645-663en_HK
dc.identifier.issn0002-9297en_HK
dc.identifier.urihttp://hdl.handle.net/10722/42289-
dc.description.abstractTo facilitate mapping of the cystic fibrosis locus (CF) and to isolate the corresponding gene, we have screened a flow-sorted chromosome 7-specific library for additional DNA markers in the 7q31-q32 region. Unique ('single-copy') DNA segments were selected from the library and used in hybridization analysis with a panel of somatic cell hybrids containing various portions of human chromosome 7 and patient cell lines with deletion of this chromosome. A total of 258 chromosome 7-specific single-copy DNA segments were identified, and most of them localized to subregions. Fifty three of these corresponded to DNA sequences in the 7q31-q32 region. Family and physical mapping studies showed that two of the DNA markers, D7S122 and D7S340, are in close linkage with CF. The data also showed that D7S122 and D7S340 map between MET and D7S8, the two genetic markers known to be on opposite sides of CF. The study thus reaffirms the general strategy in approaching a disease locus on the basis of chromosome location.en_HK
dc.format.extent3656109 bytes-
dc.format.extent30208 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.languageengen_HK
dc.publisherCell Press. The Journal's web site is located at http://www.cell.com/AJHG/en_HK
dc.relation.ispartofAmerican Journal of Human Geneticsen_HK
dc.rightsAmerican journal of human genetics. Copyright © University of Chicago Press.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshChromosome bandingen_HK
dc.subject.meshChromosomes, human, pair 7en_HK
dc.subject.meshCloning, molecularen_HK
dc.subject.meshCystic fibrosis - geneticsen_HK
dc.subject.meshDna probesen_HK
dc.titleIdentification and regional localization of DNA markers on chromosome 7 for the cloning of the cystic fibrosis geneen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0002-9297&volume=43&issue=5&spage=645&epage=663&date=1988&atitle=Identification+and+regional+localization+of+DNA+markers+on+chromosome+7+for+the+cloning+of+the+cystic+fibrosis+geneen_HK
dc.identifier.emailTsui, L: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, L=rp00058en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid2903665en_HK
dc.identifier.pmcidPMC1715526-
dc.identifier.scopuseid_2-s2.0-0023700081en_HK
dc.identifier.volume43en_HK
dc.identifier.issue5en_HK
dc.identifier.spage645en_HK
dc.identifier.epage663en_HK
dc.identifier.isiWOS:A1988Q933600011-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridRommens, JM=7006884140en_HK
dc.identifier.scopusauthoridZengerling, S=6507625753en_HK
dc.identifier.scopusauthoridBurns, J=7403680971en_HK
dc.identifier.scopusauthoridMelmer, G=7003309613en_HK
dc.identifier.scopusauthoridKerem, B=35376353800en_HK
dc.identifier.scopusauthoridPlavsic, N=8314412600en_HK
dc.identifier.scopusauthoridZsiga, M=7801563333en_HK
dc.identifier.scopusauthoridKennedy, D=7403112289en_HK
dc.identifier.scopusauthoridMarkiewicz, D=7007146509en_HK
dc.identifier.scopusauthoridRozmahel, R=6701510561en_HK
dc.identifier.scopusauthoridRiordan, JR=7202229758en_HK
dc.identifier.scopusauthoridBuchwald, M=7006759922en_HK
dc.identifier.scopusauthoridTsui, L=7102754167en_HK

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