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Article: The role of the state in higher education reform: Mainland China and Taiwan

TitleThe role of the state in higher education reform: Mainland China and Taiwan
Authors
KeywordsTuberculosis
Uracil-n-glycosylase
Nested pcr
Issue Date1995
PublisherUniversity of Chicago Press. The Journal's web site is located at http://www.journals.uchicago.edu/CER/home.html
Citation
Comparative Education Review, 1995, v. 39 n. 3, p. 322-355 How to Cite?
AbstractAIMS: To evaluate the usefulness of a single-tube nested polymerase chain reaction (PCR) assay in the diagnosis of tuberculosis in 1497 pulmonary and 536 extrapulmonary specimens. METHODS: A single-tube nested PCR, utilising two sets of primers with different melting temperatures (88 degrees C for external primers; 70 degrees C for internal primers) to augment sensitivity and specificity without increasing the risk of amplicon contamination, was evaluated. Specimens were initially tested for the repetitive IS6110 sequences and if negative, retested for the universal 38 kilodalton sequence and for inhibitors. dUTP/Uracil-N-glycosylase and Instagene treatment were used to minimise contamination and the effect of inhibitors, respectively. RESULTS: Using culture as the gold standard, the overall sensitivity of the assay was 89% for pulmonary and 42% for extrapulmonary specimens. Sensitivity varied greatly with respect to sample type (92% for follow up specimens from a chest hospital and 70% for non-follow up specimens from a general hospital). The smear positivity rates were 15% for extrapulmonary specimens, and 69% and 45%, respectively, for follow up and non-follow up specimens from pulmonary sites. Specificity was 99.7%. Inhibitors were present more frequently in extrapulmonary than in pulmonary specimens (13.4% v 2.7%). CONCLUSION: Despite the high sensitivity of the PCR assay for the diagnosis of tuberculosis in pulmonary specimens, it was less effective in the extrapulmonary samples. This is probably because of the lower bacterial load in extrapulmonary specimens, the presence of more inhibitors adversely affecting the PCR assay and the higher volume of specimens used for culture.
Persistent Identifierhttp://hdl.handle.net/10722/42071
ISSN
2015 Impact Factor: 0.943
2015 SCImago Journal Rankings: 1.486

 

DC FieldValueLanguage
dc.contributor.authorLaw, WWen_HK
dc.date.accessioned2007-01-08T02:28:14Z-
dc.date.available2007-01-08T02:28:14Z-
dc.date.issued1995en_HK
dc.identifier.citationComparative Education Review, 1995, v. 39 n. 3, p. 322-355en_HK
dc.identifier.issn0010-4086en_HK
dc.identifier.urihttp://hdl.handle.net/10722/42071-
dc.description.abstractAIMS: To evaluate the usefulness of a single-tube nested polymerase chain reaction (PCR) assay in the diagnosis of tuberculosis in 1497 pulmonary and 536 extrapulmonary specimens. METHODS: A single-tube nested PCR, utilising two sets of primers with different melting temperatures (88 degrees C for external primers; 70 degrees C for internal primers) to augment sensitivity and specificity without increasing the risk of amplicon contamination, was evaluated. Specimens were initially tested for the repetitive IS6110 sequences and if negative, retested for the universal 38 kilodalton sequence and for inhibitors. dUTP/Uracil-N-glycosylase and Instagene treatment were used to minimise contamination and the effect of inhibitors, respectively. RESULTS: Using culture as the gold standard, the overall sensitivity of the assay was 89% for pulmonary and 42% for extrapulmonary specimens. Sensitivity varied greatly with respect to sample type (92% for follow up specimens from a chest hospital and 70% for non-follow up specimens from a general hospital). The smear positivity rates were 15% for extrapulmonary specimens, and 69% and 45%, respectively, for follow up and non-follow up specimens from pulmonary sites. Specificity was 99.7%. Inhibitors were present more frequently in extrapulmonary than in pulmonary specimens (13.4% v 2.7%). CONCLUSION: Despite the high sensitivity of the PCR assay for the diagnosis of tuberculosis in pulmonary specimens, it was less effective in the extrapulmonary samples. This is probably because of the lower bacterial load in extrapulmonary specimens, the presence of more inhibitors adversely affecting the PCR assay and the higher volume of specimens used for culture.en_HK
dc.format.extent2327986 bytes-
dc.format.extent25600 bytes-
dc.format.extent418870 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.format.mimetypeapplication/pdf-
dc.languageengen_HK
dc.publisherUniversity of Chicago Press. The Journal's web site is located at http://www.journals.uchicago.edu/CER/home.htmlen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsComparative Education Review. Copyright © University of Chicago Press.en_HK
dc.subjectTuberculosisen_HK
dc.subjectUracil-n-glycosylaseen_HK
dc.subjectNested pcren_HK
dc.titleThe role of the state in higher education reform: Mainland China and Taiwanen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0010-4086&volume=39&issue=3&spage=322&epage=355&date=1995&atitle=The+role+of+the+state+in+higher+education+reform:+Mainland+China+and+Taiwanen_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.hkuros15062-

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