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Article: The metIC operon involved in methionine biosynthesis in Bacillus subtilis is controlled by transcription antitermination

TitleThe metIC operon involved in methionine biosynthesis in Bacillus subtilis is controlled by transcription antitermination
Authors
Issue Date2002
PublisherSociety for General Microbiology. The Journal's web site is located at http://mic.sgmjournals.org
Citation
Microbiology, 2002, v. 148 n. pt 2, p. 507-518 How to Cite?
AbstractThere are two major pathways for methionine biosynthesis in micro-organisms. Little is known about these pathways in Bacillus subtilis. The authors assigned a function to the metI (formerly yjcI) and metC (formerly yjcJ) genes of B. subtilis by complementing Escherichia coli metB and metC mutants, analysing the phenotype of B. subtilis metI and metC mutants, and carrying out enzyme activity assays. These genes encode polypeptides belonging to the cystathionine -synthase family of proteins. Interestingly, the MetI protein has both cystathionine -synthase and O-acetylhomoserine thiolyase activities, whereas the MetC protein is a cystathionine ß-lyase. In B. subtilis, the transsulfuration and the thiolation pathways are functional in vivo. Due to its dual activity, the MetI protein participates in both pathways. The metI and metC genes form an operon, the expression of which is subject to sulfur-dependent regulation. When the sulfur source is sulfate or cysteine the transcription of this operon is high. Conversely, when the sulfur source is methionine its transcription is low. An S-box sequence, which is located upstream of the metI gene, is involved in the regulation of the metIC operon. Northern blot experiments demonstrated the existence of two transcripts: a small transcript corresponding to the premature transcription termination at the terminator present in the S-box and a large one corresponding to transcription of the complete metIC operon. When methionine levels were limiting, the amount of the full-length transcript increased. These results substantiate a model of regulation by transcription antitermination.
Persistent Identifierhttp://hdl.handle.net/10722/42040
ISSN
2015 Impact Factor: 2.268
2015 SCImago Journal Rankings: 1.144
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorAuger, Sen_HK
dc.contributor.authorYuen, WHen_HK
dc.contributor.authorDanchin, Aen_HK
dc.contributor.authorMartin-Verstraete, Ien_HK
dc.date.accessioned2007-01-08T02:27:33Z-
dc.date.available2007-01-08T02:27:33Z-
dc.date.issued2002en_HK
dc.identifier.citationMicrobiology, 2002, v. 148 n. pt 2, p. 507-518en_HK
dc.identifier.issn1350-0872en_HK
dc.identifier.urihttp://hdl.handle.net/10722/42040-
dc.description.abstractThere are two major pathways for methionine biosynthesis in micro-organisms. Little is known about these pathways in Bacillus subtilis. The authors assigned a function to the metI (formerly yjcI) and metC (formerly yjcJ) genes of B. subtilis by complementing Escherichia coli metB and metC mutants, analysing the phenotype of B. subtilis metI and metC mutants, and carrying out enzyme activity assays. These genes encode polypeptides belonging to the cystathionine -synthase family of proteins. Interestingly, the MetI protein has both cystathionine -synthase and O-acetylhomoserine thiolyase activities, whereas the MetC protein is a cystathionine ß-lyase. In B. subtilis, the transsulfuration and the thiolation pathways are functional in vivo. Due to its dual activity, the MetI protein participates in both pathways. The metI and metC genes form an operon, the expression of which is subject to sulfur-dependent regulation. When the sulfur source is sulfate or cysteine the transcription of this operon is high. Conversely, when the sulfur source is methionine its transcription is low. An S-box sequence, which is located upstream of the metI gene, is involved in the regulation of the metIC operon. Northern blot experiments demonstrated the existence of two transcripts: a small transcript corresponding to the premature transcription termination at the terminator present in the S-box and a large one corresponding to transcription of the complete metIC operon. When methionine levels were limiting, the amount of the full-length transcript increased. These results substantiate a model of regulation by transcription antitermination.en_HK
dc.format.extent336734 bytes-
dc.format.extent25600 bytes-
dc.format.extent363115 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.format.mimetypeapplication/pdf-
dc.languageengen_HK
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://mic.sgmjournals.orgen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshBacillus subtilis - genetics - metabolismen_HK
dc.subject.meshMethionine - blooden_HK
dc.subject.meshMultienzyme complexesen_HK
dc.subject.meshSaccharomyces cerevisiae proteinsen_HK
dc.subject.meshGene expression regulation, bacterialen_HK
dc.titleThe metIC operon involved in methionine biosynthesis in Bacillus subtilis is controlled by transcription antiterminationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1350-0872&volume=148&issue=pt 2&spage=507&epage=518&date=2002&atitle=The+metIC+operon+involved+in+methionine+biosynthesis+in+Bacillus+subtilis+is+controlled+by+transcription+antiterminationen_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid11832514-
dc.identifier.scopuseid_2-s2.0-0036175114-
dc.identifier.hkuros69003-
dc.identifier.isiWOS:000173748500019-

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