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Article: Molecular cytogenetic studies in rubber, Hevea brasiliensis Muell. Arg. (Euphorbiaceae)

TitleMolecular cytogenetic studies in rubber, Hevea brasiliensis Muell. Arg. (Euphorbiaceae)
Authors
KeywordsCytogenetics
Genetic mapping
Hevea
rDNA fluorescence banding
Ribosomal DNA
Rubber
Issue Date1998
PublisherN R C Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?gen
Citation
Genome, 1998, v. 41 n. 3, p. 464-467 How to Cite?
AbstractThis paper reports the start of a molecular cytogenetics programme targeting the genome of the angiosperm tree species Hevea brasiliensis Muell. Arg. (rubber, 2n = 36), a major world crop about whose genetics very little is known. A metaphase karyotype of rubber is presented. In situ hybidization with the probe pTa71 for ribosomal DNA (rDNA) shows that there are four sites of probe hybidization occurring on two pairs of chromosomes called chromosomes 6 and 7 carrying sites NOR-1 and NOR-2, respectively. An examination of meristematic interphase nuclei shows that all four loci have the potential to be partially decondensed at interphase, although in many nuclei one or more loci appear fully condensed and apparently inactive. The probe pXVI revealed a single pair of chromosomes carrying 5S rDNA. The probes pTa71 and pXVI provide cytogenetic markers for three pairs of chromosomes that will be of use in genetic mapping programmes. The rubber chromosomes also have telomere sequences that hybridize with the Arabidopsis consensus sequence TTTAGGG. With the exception of the satellite region containing rDNA, which fluoresces brightly with chromomycin A3, fluorescence banding showed that there is no strong demarcation of the genome into GC- and AT-rich regions, as occurs in mammalian genomes.
Persistent Identifierhttp://hdl.handle.net/10722/42022
ISSN
2015 Impact Factor: 1.356
2015 SCImago Journal Rankings: 0.666
References

 

DC FieldValueLanguage
dc.contributor.authorLeitch, ARen_HK
dc.contributor.authorLim, KYen_HK
dc.contributor.authorLeitch, IJen_HK
dc.contributor.authorO'Neill, Men_HK
dc.contributor.authorChye, Men_HK
dc.contributor.authorLow, Fen_HK
dc.date.accessioned2007-01-08T02:27:09Z-
dc.date.available2007-01-08T02:27:09Z-
dc.date.issued1998en_HK
dc.identifier.citationGenome, 1998, v. 41 n. 3, p. 464-467en_HK
dc.identifier.issn0831-2796en_HK
dc.identifier.urihttp://hdl.handle.net/10722/42022-
dc.description.abstractThis paper reports the start of a molecular cytogenetics programme targeting the genome of the angiosperm tree species Hevea brasiliensis Muell. Arg. (rubber, 2n = 36), a major world crop about whose genetics very little is known. A metaphase karyotype of rubber is presented. In situ hybidization with the probe pTa71 for ribosomal DNA (rDNA) shows that there are four sites of probe hybidization occurring on two pairs of chromosomes called chromosomes 6 and 7 carrying sites NOR-1 and NOR-2, respectively. An examination of meristematic interphase nuclei shows that all four loci have the potential to be partially decondensed at interphase, although in many nuclei one or more loci appear fully condensed and apparently inactive. The probe pXVI revealed a single pair of chromosomes carrying 5S rDNA. The probes pTa71 and pXVI provide cytogenetic markers for three pairs of chromosomes that will be of use in genetic mapping programmes. The rubber chromosomes also have telomere sequences that hybridize with the Arabidopsis consensus sequence TTTAGGG. With the exception of the satellite region containing rDNA, which fluoresces brightly with chromomycin A3, fluorescence banding showed that there is no strong demarcation of the genome into GC- and AT-rich regions, as occurs in mammalian genomes.en_HK
dc.format.extent541166 bytes-
dc.format.extent3905 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherN R C Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?genen_HK
dc.relation.ispartofGenomeen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsGenome. Copyright © N R C Research Press.en_HK
dc.subjectCytogeneticsen_HK
dc.subjectGenetic mappingen_HK
dc.subjectHeveaen_HK
dc.subjectrDNA fluorescence bandingen_HK
dc.subjectRibosomal DNAen_HK
dc.subjectRubberen_HK
dc.titleMolecular cytogenetic studies in rubber, Hevea brasiliensis Muell. Arg. (Euphorbiaceae)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0831-2796&volume=41&issue=3&spage=464&epage=467&date=1998&atitle=Molecular+cytogenetic+studies+in+rubber,+Hevea+brasiliensis+Muell.+Arg.+(Euphorbiaceae)en_HK
dc.identifier.emailChye, M: mlchye@hkucc.hku.hken_HK
dc.identifier.authorityChye, M=rp00687en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.scopuseid_2-s2.0-0031718558en_HK
dc.identifier.hkuros35736-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031718558&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume41en_HK
dc.identifier.issue3en_HK
dc.identifier.spage464en_HK
dc.identifier.epage467en_HK
dc.publisher.placeCanadaen_HK
dc.identifier.scopusauthoridLeitch, AR=7103041005en_HK
dc.identifier.scopusauthoridLim, KY=8385227100en_HK
dc.identifier.scopusauthoridLeitch, IJ=7005926112en_HK
dc.identifier.scopusauthoridO'Neill, M=7401813146en_HK
dc.identifier.scopusauthoridChye, M=7003905460en_HK
dc.identifier.scopusauthoridLow, F=7005202699en_HK

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