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- Publisher Website: 10.1016/j.molimm.2008.05.020
- Scopus: eid_2-s2.0-48049083322
- PMID: 18602161
- WOS: WOS:000259473900013
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Article: Complement activation by PEGylated single-walled carbon nanotubes is independent of C1q and alternative pathway turnover
Title | Complement activation by PEGylated single-walled carbon nanotubes is independent of C1q and alternative pathway turnover |
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Authors | |
Keywords | Biomaterials C4 Carbon nanotubes Complement system Drug delivery system Nanomedicine SC5b-9 |
Issue Date | 2008 |
Citation | Molecular Immunology, 2008, v. 45, n. 14, p. 3797-3803 How to Cite? |
Abstract | We have investigated the interaction between long circulating poly(ethylene glycol)-stabilized single-walled carbon nanotubes (SWNTs) and the complement system. Aminopoly(ethylene glycol)5000-distearoylphosphatidylethanolamine (aminoPEG5000-DSPE) and methoxyPEG5000-DSPE coated as-grown HIPco SWNTs activated complement in undiluted normal human serum as reflected in significant rises in C4d and SC5b-9 levels, but not the alternative pathway split-product Bb, thus indicating activation exclusively through C4 cleavage. Studies in C2-depleted serum confirmed that PEGylated nanotube-mediated elevation of SC5b-9 was C4b2a convertase-dependent. With the aid of monoclonal antibodies against C1s and human serum depleted from C1q, nanotube-mediated complement activation in C1q-depleted serum was also shown to be independent of classical pathway. Nanotube-mediated C4d elevation in C1q-depleted serum, however, was inhibited by N-acetylglucosamine, Futhan (a broad-spectrum serine protease inhibitor capable of preventing complement activation through all three pathways) and anti-MASP-2 antibodies; this strongly suggests a role for activation of MASP-2 in subsequent C4 cleavage and assembly of C4b2a covertases. Intravenous injection of PEGylated nanotubes in some rats was associated with a significant rise in plasma thromboxane B2 levels, indicative of in vivo nanotube-mediated complement activation. The clinical implications of these observations are discussed. © 2008 Elsevier Ltd. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/334178 |
ISSN | 2021 Impact Factor: 4.174 2020 SCImago Journal Rankings: 1.291 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Hamad, Islam | - |
dc.contributor.author | Christy Hunter, A. | - |
dc.contributor.author | Rutt, Kenneth J. | - |
dc.contributor.author | Liu, Zhuang | - |
dc.contributor.author | Dai, Hongjie | - |
dc.contributor.author | Moein Moghimi, S. | - |
dc.date.accessioned | 2023-10-20T06:46:17Z | - |
dc.date.available | 2023-10-20T06:46:17Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | Molecular Immunology, 2008, v. 45, n. 14, p. 3797-3803 | - |
dc.identifier.issn | 0161-5890 | - |
dc.identifier.uri | http://hdl.handle.net/10722/334178 | - |
dc.description.abstract | We have investigated the interaction between long circulating poly(ethylene glycol)-stabilized single-walled carbon nanotubes (SWNTs) and the complement system. Aminopoly(ethylene glycol)5000-distearoylphosphatidylethanolamine (aminoPEG5000-DSPE) and methoxyPEG5000-DSPE coated as-grown HIPco SWNTs activated complement in undiluted normal human serum as reflected in significant rises in C4d and SC5b-9 levels, but not the alternative pathway split-product Bb, thus indicating activation exclusively through C4 cleavage. Studies in C2-depleted serum confirmed that PEGylated nanotube-mediated elevation of SC5b-9 was C4b2a convertase-dependent. With the aid of monoclonal antibodies against C1s and human serum depleted from C1q, nanotube-mediated complement activation in C1q-depleted serum was also shown to be independent of classical pathway. Nanotube-mediated C4d elevation in C1q-depleted serum, however, was inhibited by N-acetylglucosamine, Futhan (a broad-spectrum serine protease inhibitor capable of preventing complement activation through all three pathways) and anti-MASP-2 antibodies; this strongly suggests a role for activation of MASP-2 in subsequent C4 cleavage and assembly of C4b2a covertases. Intravenous injection of PEGylated nanotubes in some rats was associated with a significant rise in plasma thromboxane B2 levels, indicative of in vivo nanotube-mediated complement activation. The clinical implications of these observations are discussed. © 2008 Elsevier Ltd. All rights reserved. | - |
dc.language | eng | - |
dc.relation.ispartof | Molecular Immunology | - |
dc.subject | Biomaterials | - |
dc.subject | C4 | - |
dc.subject | Carbon nanotubes | - |
dc.subject | Complement system | - |
dc.subject | Drug delivery system | - |
dc.subject | Nanomedicine | - |
dc.subject | SC5b-9 | - |
dc.title | Complement activation by PEGylated single-walled carbon nanotubes is independent of C1q and alternative pathway turnover | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.molimm.2008.05.020 | - |
dc.identifier.pmid | 18602161 | - |
dc.identifier.scopus | eid_2-s2.0-48049083322 | - |
dc.identifier.volume | 45 | - |
dc.identifier.issue | 14 | - |
dc.identifier.spage | 3797 | - |
dc.identifier.epage | 3803 | - |
dc.identifier.isi | WOS:000259473900013 | - |