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- Publisher Website: 10.1038/s41467-022-28263-2
- WOS: WOS:000752207900036
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Article: Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening
Title | Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening |
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Authors | |
Issue Date | 2022 |
Publisher | Nature Publishing Group. The Journal's web site is located at http://www.nature.com/ncomms/index.html |
Citation | Nature Communications, 2022, v. 13 How to Cite? |
Abstract | Stomatal opening requires the provision of energy in the form of ATP for proton pumping across the guard cell (GC) plasma membrane and for associated metabolic rearrangements. The source of ATP for GCs is a matter of ongoing debate that is mainly fuelled by controversies around the ability of GC chloroplasts (GCCs) to perform photosynthesis. By imaging compartment-specific fluorescent ATP and NADPH sensor proteins in Arabidopsis, we show that GC photosynthesis is limited and mitochondria are the main source of ATP. Unlike mature mesophyll cell (MC) chloroplasts, which are impermeable to cytosolic ATP, GCCs import cytosolic ATP through NUCLEOTIDE TRANSPORTER (NTT) proteins. GCs from ntt mutants exhibit impaired abilities for starch biosynthesis and stomatal opening. Our work shows that GCs obtain ATP and carbohydrates via different routes from MCs, likely to compensate for the lower chlorophyll contents and limited photosynthesis of GCCs. |
Persistent Identifier | http://hdl.handle.net/10722/314822 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Lim, SL | - |
dc.contributor.author | Flütsch, S | - |
dc.contributor.author | LIU, J | - |
dc.contributor.author | Distefano, L | - |
dc.contributor.author | Santelia, D | - |
dc.contributor.author | Lim, BL | - |
dc.date.accessioned | 2022-08-05T09:35:14Z | - |
dc.date.available | 2022-08-05T09:35:14Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | Nature Communications, 2022, v. 13 | - |
dc.identifier.uri | http://hdl.handle.net/10722/314822 | - |
dc.description.abstract | Stomatal opening requires the provision of energy in the form of ATP for proton pumping across the guard cell (GC) plasma membrane and for associated metabolic rearrangements. The source of ATP for GCs is a matter of ongoing debate that is mainly fuelled by controversies around the ability of GC chloroplasts (GCCs) to perform photosynthesis. By imaging compartment-specific fluorescent ATP and NADPH sensor proteins in Arabidopsis, we show that GC photosynthesis is limited and mitochondria are the main source of ATP. Unlike mature mesophyll cell (MC) chloroplasts, which are impermeable to cytosolic ATP, GCCs import cytosolic ATP through NUCLEOTIDE TRANSPORTER (NTT) proteins. GCs from ntt mutants exhibit impaired abilities for starch biosynthesis and stomatal opening. Our work shows that GCs obtain ATP and carbohydrates via different routes from MCs, likely to compensate for the lower chlorophyll contents and limited photosynthesis of GCCs. | - |
dc.language | eng | - |
dc.publisher | Nature Publishing Group. The Journal's web site is located at http://www.nature.com/ncomms/index.html | - |
dc.relation.ispartof | Nature Communications | - |
dc.rights | This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/[insert DOI] | - |
dc.title | Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening | - |
dc.type | Article | - |
dc.identifier.email | Lim, SL: sheyli@hku.hk | - |
dc.identifier.email | Lim, BL: bllim@hku.hk | - |
dc.identifier.authority | Lim, BL=rp00744 | - |
dc.identifier.doi | 10.1038/s41467-022-28263-2 | - |
dc.identifier.hkuros | 334877 | - |
dc.identifier.volume | 13 | - |
dc.identifier.isi | WOS:000752207900036 | - |
dc.publisher.place | London | - |