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Article: Imaging and analysis on the interaction between human antigen-pulsed Vδ2 T cells and antigen-specific CD4 T cells

TitleImaging and analysis on the interaction between human antigen-pulsed Vδ2 T cells and antigen-specific CD4 T cells
Authors
MO, YCHEUNG, AKLLIU, YLiu, LChen, Z
KeywordsCell culture
Cell isolation
Immunology
Microscopy
Issue Date2021
PublisherElsevier (Cell Press): OAJ. The Journal's web site is located at https://www.cell.com/star-protocols/home
Citation
STAR Protocols, 2021, v. 2 n. 2, p. article no. 100453 How to Cite?
DOI: http://dx.doi.org/10.1016/j.xpro.2021.100453
AbstractThis protocol describes how to visualize surface protein-protein co-localization across a cell-cell interface between antigen-presenting γδ-T cells and CD4 T cells. By consolidating immunofluorescence assay, confocal microscopy and 3D imaging analysis, it enables assessment of interaction between cell surface proteins such as Δ42PD1 and TLR4 between co-cultured γδ-T and CD4 T cells. This protocol can be applied to study a surface protein of interest and its potential interaction with a target cell/protein at the cell-cell interface.
Persistent Identifierhttp://hdl.handle.net/10722/310167
ISSN
2666-1667
PubMed Central ID
PMC8076705

 

DC FieldValueLanguage
dc.contributor.authorMO, Y-
dc.contributor.authorCHEUNG, AKL-
dc.contributor.authorLIU, Y-
dc.contributor.authorLiu, L-
dc.contributor.authorChen, Z-
dc.date.accessioned2022-01-24T02:24:51Z-
dc.date.available2022-01-24T02:24:51Z-
dc.date.issued2021-
dc.identifier.citationSTAR Protocols, 2021, v. 2 n. 2, p. article no. 100453-
dc.identifier.issn2666-1667-
dc.identifier.urihttp://hdl.handle.net/10722/310167-
dc.description.abstractThis protocol describes how to visualize surface protein-protein co-localization across a cell-cell interface between antigen-presenting γδ-T cells and CD4 T cells. By consolidating immunofluorescence assay, confocal microscopy and 3D imaging analysis, it enables assessment of interaction between cell surface proteins such as Δ42PD1 and TLR4 between co-cultured γδ-T and CD4 T cells. This protocol can be applied to study a surface protein of interest and its potential interaction with a target cell/protein at the cell-cell interface.-
dc.languageeng-
dc.publisherElsevier (Cell Press): OAJ. The Journal's web site is located at https://www.cell.com/star-protocols/home-
dc.relation.ispartofSTAR Protocols-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectCell culture-
dc.subjectCell isolation-
dc.subjectImmunology-
dc.subjectMicroscopy-
dc.titleImaging and analysis on the interaction between human antigen-pulsed Vδ2 T cells and antigen-specific CD4 T cells-
dc.typeArticle-
dc.identifier.emailLiu, L: liuli71@hkucc.hku.hk-
dc.identifier.emailChen, Z: zchenai@hku.hk-
dc.identifier.authorityLiu, L=rp00268-
dc.identifier.authorityChen, Z=rp00243-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.xpro.2021.100453-
dc.identifier.pmid33937873-
dc.identifier.pmcidPMC8076705-
dc.identifier.scopuseid_2-s2.0-85104053760-
dc.identifier.hkuros331489-
dc.identifier.volume2-
dc.identifier.issue2-
dc.identifier.spagearticle no. 100453-
dc.identifier.epagearticle no. 100453-
dc.publisher.placeUnited States-

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