File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1016/j.ijbiomac.2021.07.198
- Scopus: eid_2-s2.0-85112569017
- PMID: 34371047
- WOS: WOS:000697936100002
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: 2D DNA nanoporous scaffold promotes osteogenic differentiation of pre-osteoblasts
Title | 2D DNA nanoporous scaffold promotes osteogenic differentiation of pre-osteoblasts |
---|---|
Authors | |
Keywords | Pre-osteoblasts Rectangular and double-crossover (DX) DNA-tiles 2D DNA nanoporous scaffolds (DNA-NPSs) Bone tissue regeneration |
Issue Date | 2021 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ijbiomac |
Citation | International Journal of Biological Macromolecules, 2021, v. 188, p. 657-669 How to Cite? |
Abstract | Biofunctional materials with nanomechanical parameters similar to bone tissue may promote the adherence, migration, proliferation, and differentiation of pre-osteoblasts. In this study, deoxyribonucleic acid (DNA) nanoporous scaffold (DNA-NPS) was synthesized by the polymerization of rectangular and double-crossover (DX) DNA tiles. The diagonally precise polymerization of nanometer-sized DNA tiles (A + B) through sticky end cohesion gave rise to a micrometer-sized porous giant-sheet material. The synthesized DNA-NPS exhibited a uniformly distributed porosity with a size of 25 ± 20 nm. The morphology, dimensions, sectional profiles, 2-dimensional (2D) layer height, texture, topology, pore size, and mechanical parameters of DNA-NPS have been characterized by atomic force microscopy (AFM). The size and zeta potential of DNA-NPS have been characterized by the zeta sizer. Cell biocompatibility, proliferation, and apoptosis have been evaluated by flow cytometry. The AFM results confirmed that the fabricated DNA-NPS was interconnected and uniformly porous, with a surface roughness of 0.125 ± 0.08035 nm. The elastic modulus of the DNA-NPS was 22.45 ± 8.65 GPa, which was comparable to that of native bone tissue. DNA-NPS facilitated pre-osteoblast adhesion, proliferation, and osteogenic differentiation. These findings indicated the potential of 2D DNA-NPS in promoting bone tissue regeneration. |
Persistent Identifier | http://hdl.handle.net/10722/302034 |
ISSN | 2021 Impact Factor: 8.025 2020 SCImago Journal Rankings: 1.140 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Baig, MMFA | - |
dc.contributor.author | Dissanayaka, WL | - |
dc.contributor.author | Zhang, C | - |
dc.date.accessioned | 2021-08-21T03:30:36Z | - |
dc.date.available | 2021-08-21T03:30:36Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | International Journal of Biological Macromolecules, 2021, v. 188, p. 657-669 | - |
dc.identifier.issn | 0141-8130 | - |
dc.identifier.uri | http://hdl.handle.net/10722/302034 | - |
dc.description.abstract | Biofunctional materials with nanomechanical parameters similar to bone tissue may promote the adherence, migration, proliferation, and differentiation of pre-osteoblasts. In this study, deoxyribonucleic acid (DNA) nanoporous scaffold (DNA-NPS) was synthesized by the polymerization of rectangular and double-crossover (DX) DNA tiles. The diagonally precise polymerization of nanometer-sized DNA tiles (A + B) through sticky end cohesion gave rise to a micrometer-sized porous giant-sheet material. The synthesized DNA-NPS exhibited a uniformly distributed porosity with a size of 25 ± 20 nm. The morphology, dimensions, sectional profiles, 2-dimensional (2D) layer height, texture, topology, pore size, and mechanical parameters of DNA-NPS have been characterized by atomic force microscopy (AFM). The size and zeta potential of DNA-NPS have been characterized by the zeta sizer. Cell biocompatibility, proliferation, and apoptosis have been evaluated by flow cytometry. The AFM results confirmed that the fabricated DNA-NPS was interconnected and uniformly porous, with a surface roughness of 0.125 ± 0.08035 nm. The elastic modulus of the DNA-NPS was 22.45 ± 8.65 GPa, which was comparable to that of native bone tissue. DNA-NPS facilitated pre-osteoblast adhesion, proliferation, and osteogenic differentiation. These findings indicated the potential of 2D DNA-NPS in promoting bone tissue regeneration. | - |
dc.language | eng | - |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ijbiomac | - |
dc.relation.ispartof | International Journal of Biological Macromolecules | - |
dc.subject | Pre-osteoblasts | - |
dc.subject | Rectangular and double-crossover (DX) DNA-tiles | - |
dc.subject | 2D DNA nanoporous scaffolds (DNA-NPSs) | - |
dc.subject | Bone tissue regeneration | - |
dc.title | 2D DNA nanoporous scaffold promotes osteogenic differentiation of pre-osteoblasts | - |
dc.type | Article | - |
dc.identifier.email | Baig, MMFA: faran@hku.hk | - |
dc.identifier.email | Dissanayaka, WL: warunad@hku.hk | - |
dc.identifier.email | Zhang, C: zhangcf@hku.hk | - |
dc.identifier.authority | Baig, MMFA=rp02755 | - |
dc.identifier.authority | Dissanayaka, WL=rp02216 | - |
dc.identifier.authority | Zhang, C=rp01408 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.ijbiomac.2021.07.198 | - |
dc.identifier.pmid | 34371047 | - |
dc.identifier.scopus | eid_2-s2.0-85112569017 | - |
dc.identifier.hkuros | 324239 | - |
dc.identifier.hkuros | 324254 | - |
dc.identifier.volume | 188 | - |
dc.identifier.spage | 657 | - |
dc.identifier.epage | 669 | - |
dc.identifier.isi | WOS:000697936100002 | - |
dc.publisher.place | Netherlands | - |