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Conference Paper: Capillary-driven flow microfluidics devices for point-of-care diagnostics
Title | Capillary-driven flow microfluidics devices for point-of-care diagnostics |
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Authors | |
Issue Date | 2021 |
Publisher | Fundació Scito. |
Citation | Proceedings of Emerging Investigators in Microfluidics Conference (EIMC), Online Conference, Spain, 20-21 July 2021 How to Cite? |
Abstract | Capillary-driven flow microfluidics offers the ability of loading samples into microchannels without the requirement for external pumping mechanisms. Microfluidics devices can be coated with specific reagents and a simple dipping into a fluid sample could trigger a reaction in between reagents and analytes of interest. There is a great potential to explore capillary-driven flow microfluidics and utilize it for point-of-care (POC) diagnostics e.g. for the prevention of antimicrobial resistance (AMR) in healthcare by loading bacterial/pathogenic samples into microchannels, incubating and reading the results near the patient’s bedside [1]. Several such devices have been developed for detection of biomarkers and AMR testing [1-3] along with smartphone detections which offer an alternative approach for portable point-of-need imaging requirements [4]. However, bacterial sample loading into microchannels could be challenging because of the surface properties of the microchannels and clogging of samples at the inlets. Recently, highly branched poly(N-isopropyl acrylamide) incorporating Nile red has been shown to provide a fluorescence signal upon binding to bacteria [5]. This paper showcases a capillary-driven flow microfluidics device (Chip-and-Dip) for loading of bacterial/pathogenic samples into microchannels for antimicrobial testing. This device offers the capability of capturing cells into microchannels that can be further treated with reagents to generate a colorimetric/fluorescent signal. The Chip-and-Dip device, fabricated with inexpensive materials and coated with these reagents, works by simply dipping the reagents-coated microfluidics chip into a sample. Here, we show a successful coating of microchannels with fluorescently labelled polymer and loading of Enterococcus Faecalis spiked in milk samples. |
Description | Oral presentation 018 |
Persistent Identifier | http://hdl.handle.net/10722/301983 |
DC Field | Value | Language |
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dc.contributor.author | Hassan, SU | - |
dc.contributor.author | Carter, S | - |
dc.contributor.author | Singh, S | - |
dc.contributor.author | Dyson, E | - |
dc.contributor.author | Rimmer, S | - |
dc.contributor.author | Zhang, X | - |
dc.date.accessioned | 2021-08-21T03:29:51Z | - |
dc.date.available | 2021-08-21T03:29:51Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Proceedings of Emerging Investigators in Microfluidics Conference (EIMC), Online Conference, Spain, 20-21 July 2021 | - |
dc.identifier.uri | http://hdl.handle.net/10722/301983 | - |
dc.description | Oral presentation 018 | - |
dc.description.abstract | Capillary-driven flow microfluidics offers the ability of loading samples into microchannels without the requirement for external pumping mechanisms. Microfluidics devices can be coated with specific reagents and a simple dipping into a fluid sample could trigger a reaction in between reagents and analytes of interest. There is a great potential to explore capillary-driven flow microfluidics and utilize it for point-of-care (POC) diagnostics e.g. for the prevention of antimicrobial resistance (AMR) in healthcare by loading bacterial/pathogenic samples into microchannels, incubating and reading the results near the patient’s bedside [1]. Several such devices have been developed for detection of biomarkers and AMR testing [1-3] along with smartphone detections which offer an alternative approach for portable point-of-need imaging requirements [4]. However, bacterial sample loading into microchannels could be challenging because of the surface properties of the microchannels and clogging of samples at the inlets. Recently, highly branched poly(N-isopropyl acrylamide) incorporating Nile red has been shown to provide a fluorescence signal upon binding to bacteria [5]. This paper showcases a capillary-driven flow microfluidics device (Chip-and-Dip) for loading of bacterial/pathogenic samples into microchannels for antimicrobial testing. This device offers the capability of capturing cells into microchannels that can be further treated with reagents to generate a colorimetric/fluorescent signal. The Chip-and-Dip device, fabricated with inexpensive materials and coated with these reagents, works by simply dipping the reagents-coated microfluidics chip into a sample. Here, we show a successful coating of microchannels with fluorescently labelled polymer and loading of Enterococcus Faecalis spiked in milk samples. | - |
dc.language | eng | - |
dc.publisher | Fundació Scito. | - |
dc.relation.ispartof | Proceedings of the Emerging Investigators in Microfluidics Conference (EIMC) | - |
dc.title | Capillary-driven flow microfluidics devices for point-of-care diagnostics | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Hassan, SU: suhassan@hku.hk | - |
dc.identifier.authority | Hassan, SU=rp02857 | - |
dc.identifier.doi | 10.29363/nanoge.eimc.2021.018 | - |
dc.identifier.hkuros | 324375 | - |
dc.publisher.place | València | - |