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- Publisher Website: 10.4049/jimmunol.1300111
- Scopus: eid_2-s2.0-84881450402
- PMID: 23858032
- WOS: WOS:000322632900013
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Article: Multiplex and genome-wide analyses reveal distinctive properties of KIR+ and CD56+ T cells in human blood
Title | Multiplex and genome-wide analyses reveal distinctive properties of KIR<sup>+</sup> and CD56<sup>+</sup> T cells in human blood |
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Authors | |
Issue Date | 2013 |
Citation | Journal of Immunology, 2013, v. 191, n. 4, p. 1625-1636 How to Cite? |
Abstract | Killer cell Ig-like receptors (KIRs) on NK cells have been linked to a wide spectrum of health conditions such as chronic infections, autoimmune diseases, pregnancy complications, cancers, and transplant failures. A small subset of effector memory T cells also expresses KIRs. In this study, we use modern analytic tools including genome-wide and multiplex molecular, phenotypic, and functional assays to characterize the KIR+ T cells in human blood. We find that KIR+ T cells primarily reside in the CD56+ T population that is distinctively DNAM-1high with a genome-wide quiescent transcriptome, short telomere, and limited TCR excision circles. During CMV reactivation in bone marrow transplant recipients, KIR+CD56 + T cells rapidly expanded in realtime but not KIR+CD562 T cells or KIR+ NK cells. In CMV+ asymptomatic donors, as much as 50% of CD56+ T cells are KIR+, and most are distinguishably KIR2DL2/3+NKG2C+CD57+. Functionally, the KIR+CD56+ T cell subset lyses cancer cells and CMVpp65-pulsed target cells in a dual KIR-dependent and TCR-dependent manner. Analysis of metabolic transcriptome confirms the immunological memory status of KIR+CD56+ T cells in contrast to KIR -CD56+ T cells that are more active in energy metabolism and effector differentiation. KIR-CD56+ T cells have >25-fold higher level of expression of RORC than the KIR+ counterpart and are a previously unknown producer of IL-13 rather than IL-17 in multiplex cytokine arrays. Our data provide fundamental insights into KIR+ T cells biologically and clinically. © 2013 by The American Association of Immunologists, Inc. |
Persistent Identifier | http://hdl.handle.net/10722/294468 |
ISSN | 2023 Impact Factor: 3.6 2023 SCImago Journal Rankings: 1.558 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Chan, Wing Keung | - |
dc.contributor.author | Rujkijyanont, Piya | - |
dc.contributor.author | Neale, Geoffrey | - |
dc.contributor.author | Yang, Jie | - |
dc.contributor.author | Bari, Rafijul | - |
dc.contributor.author | Gupta, Neha Das | - |
dc.contributor.author | Holladay, Martha | - |
dc.contributor.author | Rooney, Barbara | - |
dc.contributor.author | Leung, Wing | - |
dc.date.accessioned | 2020-12-03T08:22:48Z | - |
dc.date.available | 2020-12-03T08:22:48Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Journal of Immunology, 2013, v. 191, n. 4, p. 1625-1636 | - |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.uri | http://hdl.handle.net/10722/294468 | - |
dc.description.abstract | Killer cell Ig-like receptors (KIRs) on NK cells have been linked to a wide spectrum of health conditions such as chronic infections, autoimmune diseases, pregnancy complications, cancers, and transplant failures. A small subset of effector memory T cells also expresses KIRs. In this study, we use modern analytic tools including genome-wide and multiplex molecular, phenotypic, and functional assays to characterize the KIR+ T cells in human blood. We find that KIR+ T cells primarily reside in the CD56+ T population that is distinctively DNAM-1high with a genome-wide quiescent transcriptome, short telomere, and limited TCR excision circles. During CMV reactivation in bone marrow transplant recipients, KIR+CD56 + T cells rapidly expanded in realtime but not KIR+CD562 T cells or KIR+ NK cells. In CMV+ asymptomatic donors, as much as 50% of CD56+ T cells are KIR+, and most are distinguishably KIR2DL2/3+NKG2C+CD57+. Functionally, the KIR+CD56+ T cell subset lyses cancer cells and CMVpp65-pulsed target cells in a dual KIR-dependent and TCR-dependent manner. Analysis of metabolic transcriptome confirms the immunological memory status of KIR+CD56+ T cells in contrast to KIR -CD56+ T cells that are more active in energy metabolism and effector differentiation. KIR-CD56+ T cells have >25-fold higher level of expression of RORC than the KIR+ counterpart and are a previously unknown producer of IL-13 rather than IL-17 in multiplex cytokine arrays. Our data provide fundamental insights into KIR+ T cells biologically and clinically. © 2013 by The American Association of Immunologists, Inc. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of Immunology | - |
dc.title | Multiplex and genome-wide analyses reveal distinctive properties of KIR<sup>+</sup> and CD56<sup>+</sup> T cells in human blood | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.4049/jimmunol.1300111 | - |
dc.identifier.pmid | 23858032 | - |
dc.identifier.pmcid | PMC4275795 | - |
dc.identifier.scopus | eid_2-s2.0-84881450402 | - |
dc.identifier.volume | 191 | - |
dc.identifier.issue | 4 | - |
dc.identifier.spage | 1625 | - |
dc.identifier.epage | 1636 | - |
dc.identifier.eissn | 1550-6606 | - |
dc.identifier.isi | WOS:000322632900013 | - |
dc.identifier.issnl | 0022-1767 | - |