File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Cognitive impairment and transcriptomic profile in hippocampus of young mice after multiple neonatal exposures to sevoflurane

TitleCognitive impairment and transcriptomic profile in hippocampus of young mice after multiple neonatal exposures to sevoflurane
Authors
Keywordssevoflurane
cognitive function
hippocampus
RNA sequencing
differentially expressed genes
Issue Date2019
PublisherImpact Journals LLC. The Journal's web site is located at http://www.impactaging.com
Citation
Aging, 2019, v. 11 n. 19, p. 8386-8417 How to Cite?
AbstractChildren with repeated inhalational anesthesia may develop cognitive disorders. This study aimed to investigate the transcriptome-wide response of hippocampus in young mice that had been exposed to multiple sevoflurane in the neonatal period. Mice received 3% sevoflurane for 2 h on postnatal day (PND) 6, 8, and 10, followed by arterial blood gas test on PND 10, behavioral experiments on PND 31–36, and RNA sequencing (RNA-seq) of hippocampus on PND 37. Functional annotation and protein-protein interaction analyses of differentially expressed genes (DEGs) and quantitative reverse transcription polymerase chain reaction (qPCR) were performed. Neonatal sevoflurane exposures induced cognitive and social behavior disorders in young mice. RNA-seq identified a total of 314 DEGs. Several enriched biological processes (ion channels, brain development, learning, and memory) and signaling pathways (oxytocin signaling pathway and glutamatergic, cholinergic, and GABAergic synapses) were highlighted. As hub-proteins, Pten was involved in nervous system development, synapse assembly, learning, memory, and behaviors, Nos3 and Pik3cd in oxytocin signaling pathway, and Cdk16 in exocytosis and phosphorylation. Some top DEGs were validated by qPCR. This study revealed a transcriptome-wide profile in mice hippocampus after multiple neonatal exposures to sevoflurane, promoting better understanding of underlying mechanisms and investigation of preventive strategies.
Descriptioneid_2-s2.0-85073641084
Persistent Identifierhttp://hdl.handle.net/10722/293258
ISSN
2019 Impact Factor: 4.831
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorSong, SY-
dc.contributor.authorMeng, XW-
dc.contributor.authorXia, Z-
dc.contributor.authorLiu, H-
dc.contributor.authorZhang, J-
dc.contributor.authorChen, QC-
dc.contributor.authorLiu, HY-
dc.contributor.authorJi, FH-
dc.contributor.authorPeng, K-
dc.date.accessioned2020-11-23T08:14:08Z-
dc.date.available2020-11-23T08:14:08Z-
dc.date.issued2019-
dc.identifier.citationAging, 2019, v. 11 n. 19, p. 8386-8417-
dc.identifier.issn1945-4589-
dc.identifier.urihttp://hdl.handle.net/10722/293258-
dc.descriptioneid_2-s2.0-85073641084-
dc.description.abstractChildren with repeated inhalational anesthesia may develop cognitive disorders. This study aimed to investigate the transcriptome-wide response of hippocampus in young mice that had been exposed to multiple sevoflurane in the neonatal period. Mice received 3% sevoflurane for 2 h on postnatal day (PND) 6, 8, and 10, followed by arterial blood gas test on PND 10, behavioral experiments on PND 31–36, and RNA sequencing (RNA-seq) of hippocampus on PND 37. Functional annotation and protein-protein interaction analyses of differentially expressed genes (DEGs) and quantitative reverse transcription polymerase chain reaction (qPCR) were performed. Neonatal sevoflurane exposures induced cognitive and social behavior disorders in young mice. RNA-seq identified a total of 314 DEGs. Several enriched biological processes (ion channels, brain development, learning, and memory) and signaling pathways (oxytocin signaling pathway and glutamatergic, cholinergic, and GABAergic synapses) were highlighted. As hub-proteins, Pten was involved in nervous system development, synapse assembly, learning, memory, and behaviors, Nos3 and Pik3cd in oxytocin signaling pathway, and Cdk16 in exocytosis and phosphorylation. Some top DEGs were validated by qPCR. This study revealed a transcriptome-wide profile in mice hippocampus after multiple neonatal exposures to sevoflurane, promoting better understanding of underlying mechanisms and investigation of preventive strategies.-
dc.languageeng-
dc.publisherImpact Journals LLC. The Journal's web site is located at http://www.impactaging.com-
dc.relation.ispartofAging-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectsevoflurane-
dc.subjectcognitive function-
dc.subjecthippocampus-
dc.subjectRNA sequencing-
dc.subjectdifferentially expressed genes-
dc.titleCognitive impairment and transcriptomic profile in hippocampus of young mice after multiple neonatal exposures to sevoflurane-
dc.typeArticle-
dc.identifier.emailXia, Z: zyxia@hkucc.hku.hk-
dc.identifier.authorityXia, Z=rp00532-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.18632/aging.102326-
dc.identifier.pmid31582589-
dc.identifier.pmcidPMC6814607-
dc.identifier.hkuros319797-
dc.identifier.volume11-
dc.identifier.issue19-
dc.identifier.spage8386-
dc.identifier.epage8417-
dc.publisher.placeUnited States-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats