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- Publisher Website: 10.1002/stem.2928
- Scopus: eid_2-s2.0-85065034571
- PMID: 30353613
- WOS: WOS:000466952000008
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Article: Xist Intron 1 Repression by Transcriptional-Activator-Like Effectors Designer Transcriptional Factor Improves Somatic Cell Reprogramming in Mice
Title | Xist Intron 1 Repression by Transcriptional-Activator-Like Effectors Designer Transcriptional Factor Improves Somatic Cell Reprogramming in Mice |
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Authors | |
Keywords | embryo enhancer region epigenetics female gene |
Issue Date | 2019 |
Publisher | AlphaMed Press. The Journal's web site is located at http://www.stemcells.com |
Citation | Stem Cells, 2019, v. 37 n. 5, p. 599-608 How to Cite? |
Abstract | Xist is the master regulator of X chromosome inactivation. In order to further understand the Xist locus in the reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) and in somatic cell nuclear transfer (SCNT), we tested transcription‐activator‐like effectors‐based designer transcriptional factors (dTFs), which were specific to numerous regions at the Xist locus. We report that the selected dTF repressor 6 (R6) binding the intron 1 of Xist, which caused higher H3K9me3 followed by X chromosome opening and repression of X‐linked genes in mouse embryonic fibroblasts, rather than affecting Xist expression, substantially improved the iPSC generation and the SCNT preimplantation embryo development. Conversely, the dTF activator targeting the same genomic region of R6 decreased iPSC formation and blocked SCNT‐embryo development. These results thus uncover the critical requirement for the Xist locus in epigenetic resetting, which is not directly related to Xist transcription. This may provide a unique route to improving the reprogramming. Stem Cells 2019;37:599–608 |
Description | Link to Free access |
Persistent Identifier | http://hdl.handle.net/10722/290510 |
ISSN | 2023 Impact Factor: 4.0 2023 SCImago Journal Rankings: 1.396 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Zhang, J | - |
dc.contributor.author | Gao, X | - |
dc.contributor.author | Yang, J | - |
dc.contributor.author | Fan, X | - |
dc.contributor.author | Wang, W | - |
dc.contributor.author | Liang, Y | - |
dc.contributor.author | Fan, L | - |
dc.contributor.author | Han, H | - |
dc.contributor.author | Xu, X | - |
dc.contributor.author | Tang, F | - |
dc.contributor.author | Bao, S | - |
dc.contributor.author | Liu, P | - |
dc.contributor.author | Li, X | - |
dc.date.accessioned | 2020-11-02T05:43:16Z | - |
dc.date.available | 2020-11-02T05:43:16Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Stem Cells, 2019, v. 37 n. 5, p. 599-608 | - |
dc.identifier.issn | 1066-5099 | - |
dc.identifier.uri | http://hdl.handle.net/10722/290510 | - |
dc.description | Link to Free access | - |
dc.description.abstract | Xist is the master regulator of X chromosome inactivation. In order to further understand the Xist locus in the reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) and in somatic cell nuclear transfer (SCNT), we tested transcription‐activator‐like effectors‐based designer transcriptional factors (dTFs), which were specific to numerous regions at the Xist locus. We report that the selected dTF repressor 6 (R6) binding the intron 1 of Xist, which caused higher H3K9me3 followed by X chromosome opening and repression of X‐linked genes in mouse embryonic fibroblasts, rather than affecting Xist expression, substantially improved the iPSC generation and the SCNT preimplantation embryo development. Conversely, the dTF activator targeting the same genomic region of R6 decreased iPSC formation and blocked SCNT‐embryo development. These results thus uncover the critical requirement for the Xist locus in epigenetic resetting, which is not directly related to Xist transcription. This may provide a unique route to improving the reprogramming. Stem Cells 2019;37:599–608 | - |
dc.language | eng | - |
dc.publisher | AlphaMed Press. The Journal's web site is located at http://www.stemcells.com | - |
dc.relation.ispartof | Stem Cells | - |
dc.subject | embryo | - |
dc.subject | enhancer region | - |
dc.subject | epigenetics | - |
dc.subject | female | - |
dc.subject | gene | - |
dc.title | Xist Intron 1 Repression by Transcriptional-Activator-Like Effectors Designer Transcriptional Factor Improves Somatic Cell Reprogramming in Mice | - |
dc.type | Article | - |
dc.identifier.email | Liu, P: pliu88@hku.hk | - |
dc.identifier.authority | Liu, P=rp02328 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1002/stem.2928 | - |
dc.identifier.pmid | 30353613 | - |
dc.identifier.scopus | eid_2-s2.0-85065034571 | - |
dc.identifier.hkuros | 317867 | - |
dc.identifier.volume | 37 | - |
dc.identifier.issue | 5 | - |
dc.identifier.spage | 599 | - |
dc.identifier.epage | 608 | - |
dc.identifier.isi | WOS:000466952000008 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1066-5099 | - |