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Article: Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018

TitleDiscovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018
Authors
Keywordshuman coronavirus NL63
new subgenoty
pepneumonia
whole-genome sequencing
phylogenetic analysis
Issue Date2020
PublisherTaylor & Francis Group, on behalf of Shanghai ShangyixunCultural Communication Co., Ltd. The Journal's web site is located at https://www.tandfonline.com/toc/temi20/current
Citation
Emerging Microbes & Infections, 2020, v. 9 n. 1, p. 246-255 How to Cite?
AbstractHuman coronavirus NL63 (HCoV-NL63) is primarily associated with common cold in children, elderly and immunocompromised individuals. Outbreaks caused by HCoV-NL63 are rare. Here we report a cluster of HCoV-NL63 cases with severe lower respiratory tract infection that arose in Guangzhou, China, in 2018. Twenty-three hospitalized children were confirmed to be HCoV-NL63 positive, and most of whom were hospitalized with severe pneumonia or acute bronchitis. Whole genomes of HCoV-NL63 were obtained using next-generation sequencing. Phylogenetic and single amino acid polymorphism analyses showed that this outbreak was associated with two subgenotypes (C3 and B) of HCoV-NL63. Half of patients were identified to be related to a new subgenotype C3. One unique amino acid mutation at I507 L in spike protein receptor binding domain (RBD) was detected, which segregated this subgenotype C3 from other known subgenotypes. Pseudotyped virus bearing the I507 L mutation in RBD showed enhanced entry into host cells as compared to the prototype virus. This study proved that HCoV-NL63 was undergoing continuous mutation and has the potential to cause severe lower respiratory disease in humans.
Persistent Identifierhttp://hdl.handle.net/10722/290019
ISSN
2018 Impact Factor: 6.212
2015 SCImago Journal Rankings: 1.774
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWang, Y-
dc.contributor.authorLi, X-
dc.contributor.authorLiu, W-
dc.contributor.authorGan, M-
dc.contributor.authorZhang, L-
dc.contributor.authorWang, J-
dc.contributor.authorZhang, Z-
dc.contributor.authorZhu, A-
dc.contributor.authorLi, F-
dc.contributor.authorSun, J-
dc.contributor.authorZhang, G-
dc.contributor.authorZhuang, Z-
dc.contributor.authorLuo, J-
dc.contributor.authorChen, D-
dc.contributor.authorQiu, S-
dc.contributor.authorZhang, L-
dc.contributor.authorXu, D-
dc.contributor.authorMok, CKP-
dc.contributor.authorZhang, F-
dc.contributor.authorZhao, J-
dc.contributor.authorZhou, R-
dc.contributor.authorZhao, J-
dc.date.accessioned2020-10-22T08:20:49Z-
dc.date.available2020-10-22T08:20:49Z-
dc.date.issued2020-
dc.identifier.citationEmerging Microbes & Infections, 2020, v. 9 n. 1, p. 246-255-
dc.identifier.issn2222-1751-
dc.identifier.urihttp://hdl.handle.net/10722/290019-
dc.description.abstractHuman coronavirus NL63 (HCoV-NL63) is primarily associated with common cold in children, elderly and immunocompromised individuals. Outbreaks caused by HCoV-NL63 are rare. Here we report a cluster of HCoV-NL63 cases with severe lower respiratory tract infection that arose in Guangzhou, China, in 2018. Twenty-three hospitalized children were confirmed to be HCoV-NL63 positive, and most of whom were hospitalized with severe pneumonia or acute bronchitis. Whole genomes of HCoV-NL63 were obtained using next-generation sequencing. Phylogenetic and single amino acid polymorphism analyses showed that this outbreak was associated with two subgenotypes (C3 and B) of HCoV-NL63. Half of patients were identified to be related to a new subgenotype C3. One unique amino acid mutation at I507 L in spike protein receptor binding domain (RBD) was detected, which segregated this subgenotype C3 from other known subgenotypes. Pseudotyped virus bearing the I507 L mutation in RBD showed enhanced entry into host cells as compared to the prototype virus. This study proved that HCoV-NL63 was undergoing continuous mutation and has the potential to cause severe lower respiratory disease in humans.-
dc.languageeng-
dc.publisherTaylor & Francis Group, on behalf of Shanghai ShangyixunCultural Communication Co., Ltd. The Journal's web site is located at https://www.tandfonline.com/toc/temi20/current-
dc.relation.ispartofEmerging Microbes & Infections-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjecthuman coronavirus NL63-
dc.subjectnew subgenoty-
dc.subjectpepneumonia-
dc.subjectwhole-genome sequencing-
dc.subjectphylogenetic analysis-
dc.titleDiscovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018-
dc.typeArticle-
dc.identifier.emailMok, CKP: ch02mkp@hkucc.hku.hk-
dc.identifier.authorityMok, CKP=rp01805-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1080/22221751.2020.1717999-
dc.identifier.pmid31996093-
dc.identifier.pmcidPMC7034077-
dc.identifier.scopuseid_2-s2.0-85078691103-
dc.identifier.hkuros317013-
dc.identifier.volume9-
dc.identifier.issue1-
dc.identifier.spage246-
dc.identifier.epage255-
dc.identifier.isiWOS:000510237900001-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl2222-1751-

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