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Article: Oil-mediated high-throughput generation and sorting of water-in-water droplets
Title | Oil-mediated high-throughput generation and sorting of water-in-water droplets |
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Authors | |
Keywords | Aqueous two phase system Biological assays Double emulsions Droplet generation Generation frequency |
Issue Date | 2020 |
Publisher | Nature Publishing Group, in partnership with the Aerospace Information Research Institute, Chinese Academy of Sciences. The Journal's web site is located at http://www.nature.com/micronano/ |
Citation | Microsystems & Nanoengineering, 2020, v. 6 n. 1, p. article no. 70 How to Cite? |
Abstract | Aqueous two-phase system (ATPS) droplets have demonstrated superior compatibility over conventional water-in-oil droplets for various biological assays. However, the ultralow interfacial tension hampers efficient and stable droplet generation, limiting further development and more extensive use of such approaches. Here, we present a simple strategy to employ oil as a transient medium for ATPS droplet generation. Two methods based on passive flow focusing and active pico-injection are demonstrated to generate water-water-oil double emulsions, achieving a high generation frequency of ~2.4 kHz. Through evaporation of the oil to break the double emulsions, the aqueous core can be released to form uniform-sized water-in-water droplets. Moreover, this technique can be used to fabricate aqueous microgels, and the introduction of the oil medium enables integration of droplet sorting to produce single-cell-laden hydrogels with a harvest rate of over 90%. We believe that the demonstrated high-throughput generation and sorting of ATPS droplets represent an important tool to advance droplet-based tissue engineering and single-cell analyses. |
Persistent Identifier | http://hdl.handle.net/10722/289714 |
ISSN | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | NAN, L | - |
dc.contributor.author | CAO, Y | - |
dc.contributor.author | YUAN, S | - |
dc.contributor.author | Shum, HC | - |
dc.date.accessioned | 2020-10-22T08:16:25Z | - |
dc.date.available | 2020-10-22T08:16:25Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Microsystems & Nanoengineering, 2020, v. 6 n. 1, p. article no. 70 | - |
dc.identifier.issn | 2096-1030 | - |
dc.identifier.uri | http://hdl.handle.net/10722/289714 | - |
dc.description.abstract | Aqueous two-phase system (ATPS) droplets have demonstrated superior compatibility over conventional water-in-oil droplets for various biological assays. However, the ultralow interfacial tension hampers efficient and stable droplet generation, limiting further development and more extensive use of such approaches. Here, we present a simple strategy to employ oil as a transient medium for ATPS droplet generation. Two methods based on passive flow focusing and active pico-injection are demonstrated to generate water-water-oil double emulsions, achieving a high generation frequency of ~2.4 kHz. Through evaporation of the oil to break the double emulsions, the aqueous core can be released to form uniform-sized water-in-water droplets. Moreover, this technique can be used to fabricate aqueous microgels, and the introduction of the oil medium enables integration of droplet sorting to produce single-cell-laden hydrogels with a harvest rate of over 90%. We believe that the demonstrated high-throughput generation and sorting of ATPS droplets represent an important tool to advance droplet-based tissue engineering and single-cell analyses. | - |
dc.language | eng | - |
dc.publisher | Nature Publishing Group, in partnership with the Aerospace Information Research Institute, Chinese Academy of Sciences. The Journal's web site is located at http://www.nature.com/micronano/ | - |
dc.relation.ispartof | Microsystems & Nanoengineering | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Aqueous two phase system | - |
dc.subject | Biological assays | - |
dc.subject | Double emulsions | - |
dc.subject | Droplet generation | - |
dc.subject | Generation frequency | - |
dc.title | Oil-mediated high-throughput generation and sorting of water-in-water droplets | - |
dc.type | Article | - |
dc.identifier.email | Shum, HC: ashum@hku.hk | - |
dc.identifier.authority | Shum, HC=rp01439 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1038/s41378-020-0180-0 | - |
dc.identifier.scopus | eid_2-s2.0-85090283291 | - |
dc.identifier.hkuros | 317454 | - |
dc.identifier.volume | 6 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | article no. 70 | - |
dc.identifier.epage | article no. 70 | - |
dc.identifier.isi | WOS:000570228600001 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 2055-7434 | - |