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Article: Integration of biochemical and topographic cues for the formation and spatial distribution of invadosomes in nasopharyngeal epithelial cells

TitleIntegration of biochemical and topographic cues for the formation and spatial distribution of invadosomes in nasopharyngeal epithelial cells
Authors
KeywordsInvadosomes
Nasopharyngeal Carcinoma (NPC)
Engineered platform
Topographical Pattern
Biomimetic system
Issue Date2020
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/actabiomat
Citation
Acta Biomaterialia, 2020, v. 101, p. 168-182 How to Cite?
AbstractInvadosomes are invasive protrusions generated by cells which can secrete matrix metalloproteinases for focal digestion of extracellular matrix. They also aid invasive cancer cells in their transmigration through vascular endothelium. However, how the physical and chemical cues in a three-dimensional (3D) system signal the spatial localization of invadosomes remains largely unknown. Here we study the topographic guidance of invadosome formation in invasive nasopharyngeal cells under the stimulation of an inflammatory cytokine, TGF-β1, using engineered gratings with different width and depth. We first report that TGF-β1 can act as an external signal to upregulate the formation of invadosomes with a random distribution on a plane 2D surface. When the cells were seeded on parallel 3D gratings of 5 µm width and 1 µm depth, most of the invadosomes aligned to the edges of the gratings, indicating a topographic cue to the control of invadosome localization. While the number of invadosomes per cell were not upregulated when the cells were seeded on 3D topography, guidance of invadosomes localization to edges is correlated with cell migration directionality on 1 µm deep gratings. Invadosomes preferentially form at edges when the cells move at a lower speed and are guided along narrow gratings. The invadosomes forming at 3D edges also have a longer half-life than those forming on a plane surface. These data suggest that there are integrated biochemical and 3D geometric cues underlying the spatial regulation of invasive structures so as to elicit efficient invasion or metastasis of cells.
Persistent Identifierhttp://hdl.handle.net/10722/287924
ISSN
2019 Impact Factor: 7.242
2015 SCImago Journal Rankings: 2.020

 

DC FieldValueLanguage
dc.contributor.authorTsang, CM-
dc.contributor.authorLiu, ZY-
dc.contributor.authorZhang, W-
dc.contributor.authorYou, C-
dc.contributor.authorJones, GE-
dc.contributor.authorTsao, SW-
dc.contributor.authorPang, SW-
dc.date.accessioned2020-10-05T12:05:14Z-
dc.date.available2020-10-05T12:05:14Z-
dc.date.issued2020-
dc.identifier.citationActa Biomaterialia, 2020, v. 101, p. 168-182-
dc.identifier.issn1742-7061-
dc.identifier.urihttp://hdl.handle.net/10722/287924-
dc.description.abstractInvadosomes are invasive protrusions generated by cells which can secrete matrix metalloproteinases for focal digestion of extracellular matrix. They also aid invasive cancer cells in their transmigration through vascular endothelium. However, how the physical and chemical cues in a three-dimensional (3D) system signal the spatial localization of invadosomes remains largely unknown. Here we study the topographic guidance of invadosome formation in invasive nasopharyngeal cells under the stimulation of an inflammatory cytokine, TGF-β1, using engineered gratings with different width and depth. We first report that TGF-β1 can act as an external signal to upregulate the formation of invadosomes with a random distribution on a plane 2D surface. When the cells were seeded on parallel 3D gratings of 5 µm width and 1 µm depth, most of the invadosomes aligned to the edges of the gratings, indicating a topographic cue to the control of invadosome localization. While the number of invadosomes per cell were not upregulated when the cells were seeded on 3D topography, guidance of invadosomes localization to edges is correlated with cell migration directionality on 1 µm deep gratings. Invadosomes preferentially form at edges when the cells move at a lower speed and are guided along narrow gratings. The invadosomes forming at 3D edges also have a longer half-life than those forming on a plane surface. These data suggest that there are integrated biochemical and 3D geometric cues underlying the spatial regulation of invasive structures so as to elicit efficient invasion or metastasis of cells.-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/actabiomat-
dc.relation.ispartofActa Biomaterialia-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectInvadosomes-
dc.subjectNasopharyngeal Carcinoma (NPC)-
dc.subjectEngineered platform-
dc.subjectTopographical Pattern-
dc.subjectBiomimetic system-
dc.titleIntegration of biochemical and topographic cues for the formation and spatial distribution of invadosomes in nasopharyngeal epithelial cells-
dc.typeArticle-
dc.identifier.emailTsang, CM: annatsan@hku.hk-
dc.identifier.emailTsao, SW: gswtsao@hku.hk-
dc.identifier.authorityTsang, CM=rp01964-
dc.identifier.authorityTsao, SW=rp00399-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.actbio.2019.10.043-
dc.identifier.pmid31683015-
dc.identifier.scopuseid_2-s2.0-85075807307-
dc.identifier.hkuros315769-
dc.identifier.volume101-
dc.identifier.spage168-
dc.identifier.epage182-
dc.publisher.placeNetherlands-

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