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Conference Paper: FluorATOM: high-throughput imaging flow cytometry for synchronized biophysical and biomolecular phenotyping

TitleFluorATOM: high-throughput imaging flow cytometry for synchronized biophysical and biomolecular phenotyping
Authors
Issue Date2020
PublisherSPIE - International Society for Optical Engineering.
Citation
Proceedings of SPIE Photonics West BIOS 2020: High-Speed Biomedical Imaging and Spectroscopy V, San Francisco, California, USA, 1-6 February 2020, v. 11250, paper nos. 112500L How to Cite?
AbstractMultiplexed asymmetric-detection time-stretch optical microscopy (multi-ATOM) has recently been developed to enable high-throughput quantitative phase imaging flow cytometry, from which single-cell biophysical properties can be measured at large scale. However, it lacks the ability to link such biophysical knowledge to biomolecular signatures at the single-cell precision for validation and correlative multi-scale single-cell analysis. We report a high-throughput multimodal system that integrates multi-ATOM with multiplexed 1-D fluorescence imaging/detection, termed FluorATOM; and applied it to perform synchronized biophysical and biomolecular phenotyping of rare breast circulating tumor cells detected in peripheral blood in a mouse xenograft at a throughput of >10,000 cell/sec.
DescriptionSession 4: Imaging Flow Cytometry
Persistent Identifierhttp://hdl.handle.net/10722/286729

 

DC FieldValueLanguage
dc.contributor.authorLee, CM-
dc.contributor.authorWang, M-
dc.contributor.authorCheuk, WYI-
dc.contributor.authorShin, VY-
dc.contributor.authorKwong, A-
dc.contributor.authorWong, KKY-
dc.contributor.authorSo, HKH-
dc.contributor.authorTsia, KKM-
dc.date.accessioned2020-09-04T13:29:31Z-
dc.date.available2020-09-04T13:29:31Z-
dc.date.issued2020-
dc.identifier.citationProceedings of SPIE Photonics West BIOS 2020: High-Speed Biomedical Imaging and Spectroscopy V, San Francisco, California, USA, 1-6 February 2020, v. 11250, paper nos. 112500L-
dc.identifier.urihttp://hdl.handle.net/10722/286729-
dc.descriptionSession 4: Imaging Flow Cytometry-
dc.description.abstractMultiplexed asymmetric-detection time-stretch optical microscopy (multi-ATOM) has recently been developed to enable high-throughput quantitative phase imaging flow cytometry, from which single-cell biophysical properties can be measured at large scale. However, it lacks the ability to link such biophysical knowledge to biomolecular signatures at the single-cell precision for validation and correlative multi-scale single-cell analysis. We report a high-throughput multimodal system that integrates multi-ATOM with multiplexed 1-D fluorescence imaging/detection, termed FluorATOM; and applied it to perform synchronized biophysical and biomolecular phenotyping of rare breast circulating tumor cells detected in peripheral blood in a mouse xenograft at a throughput of >10,000 cell/sec.-
dc.languageeng-
dc.publisherSPIE - International Society for Optical Engineering.-
dc.relation.ispartofSPIE Photonics West BIOS: v. 11250, High-Speed Biomedical Imaging and Spectroscopy, 2020-
dc.rightsSPIE Photonics West BIOS: v. 11250, High-Speed Biomedical Imaging and Spectroscopy, 2020. Copyright © SPIE - International Society for Optical Engineering.-
dc.titleFluorATOM: high-throughput imaging flow cytometry for synchronized biophysical and biomolecular phenotyping-
dc.typeConference_Paper-
dc.identifier.emailLee, CM: cmleehku@connect.hku.hk-
dc.identifier.emailCheuk, WYI: isacheuk@hku.hk-
dc.identifier.emailShin, VY: vyshin@hku.hk-
dc.identifier.emailKwong, A: avakwong@hku.hk-
dc.identifier.emailWong, KKY: kywong@eee.hku.hk-
dc.identifier.emailSo, HKH: hso@eee.hku.hk-
dc.identifier.emailTsia, KKM: tsia@hku.hk-
dc.identifier.authorityShin, VY=rp02000-
dc.identifier.authorityKwong, A=rp01734-
dc.identifier.authorityWong, KKY=rp00189-
dc.identifier.authoritySo, HKH=rp00169-
dc.identifier.authorityTsia, KKM=rp01389-
dc.identifier.doi10.1117/12.2548252-
dc.identifier.hkuros314127-
dc.identifier.volume11250-
dc.identifier.spage112500L-
dc.identifier.epage112500L-
dc.publisher.placeUnited States-

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