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- Publisher Website: 10.1038/s41467-020-17056-0
- Scopus: eid_2-s2.0-85086844355
- PMID: 32591540
- WOS: WOS:000544946500003
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Article: In planta study of photosynthesis and photorespiration using NADPH and NADH/NAD+ fluorescent protein sensors
Title | In planta study of photosynthesis and photorespiration using NADPH and NADH/NAD+ fluorescent protein sensors |
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Authors | |
Keywords | bioenergetics biomonitoring chloroplast decarboxylation herb |
Issue Date | 2020 |
Publisher | Nature Research (part of Springer Nature): Fully open access journals. The Journal's web site is located at http://www.nature.com/ncomms/index.html |
Citation | Nature Communications, 2020, v. 11, p. article no. 3238 How to Cite? |
Abstract | The challenge of monitoring in planta dynamic changes of NADP(H) and NAD(H) redox states at the subcellular level is considered a major obstacle in plant bioenergetics studies. Here, we introduced two circularly permuted yellow fluorescent protein sensors, iNAP and SoNar, into Arabidopsis thaliana to monitor the dynamic changes in NADPH and the NADH/NAD+ ratio. In the light, photosynthesis and photorespiration are linked to the redox states of NAD(P)H and NAD(P) pools in several subcellular compartments connected by the malate-OAA shuttles. We show that the photosynthetic increases in stromal NADPH and NADH/NAD+ ratio, but not ATP, disappear when glycine decarboxylation is inhibited. These observations highlight the complex interplay between chloroplasts and mitochondria during photosynthesis and support the suggestions that, under normal conditions, photorespiration supplies a large amount of NADH to mitochondria, exceeding its NADH-dissipating capacity, and the surplus NADH is exported from the mitochondria to the cytosol through the malate-OAA shuttle. |
Persistent Identifier | http://hdl.handle.net/10722/285374 |
ISSN | 2023 Impact Factor: 14.7 2023 SCImago Journal Rankings: 4.887 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | LIM, SL | - |
dc.contributor.author | VOON, CP | - |
dc.contributor.author | GUAN, X | - |
dc.contributor.author | Yang, Y | - |
dc.contributor.author | Gardestrom, P | - |
dc.contributor.author | Lim, BL | - |
dc.date.accessioned | 2020-08-18T03:52:52Z | - |
dc.date.available | 2020-08-18T03:52:52Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Nature Communications, 2020, v. 11, p. article no. 3238 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | http://hdl.handle.net/10722/285374 | - |
dc.description.abstract | The challenge of monitoring in planta dynamic changes of NADP(H) and NAD(H) redox states at the subcellular level is considered a major obstacle in plant bioenergetics studies. Here, we introduced two circularly permuted yellow fluorescent protein sensors, iNAP and SoNar, into Arabidopsis thaliana to monitor the dynamic changes in NADPH and the NADH/NAD+ ratio. In the light, photosynthesis and photorespiration are linked to the redox states of NAD(P)H and NAD(P) pools in several subcellular compartments connected by the malate-OAA shuttles. We show that the photosynthetic increases in stromal NADPH and NADH/NAD+ ratio, but not ATP, disappear when glycine decarboxylation is inhibited. These observations highlight the complex interplay between chloroplasts and mitochondria during photosynthesis and support the suggestions that, under normal conditions, photorespiration supplies a large amount of NADH to mitochondria, exceeding its NADH-dissipating capacity, and the surplus NADH is exported from the mitochondria to the cytosol through the malate-OAA shuttle. | - |
dc.language | eng | - |
dc.publisher | Nature Research (part of Springer Nature): Fully open access journals. The Journal's web site is located at http://www.nature.com/ncomms/index.html | - |
dc.relation.ispartof | Nature Communications | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | bioenergetics | - |
dc.subject | biomonitoring | - |
dc.subject | chloroplast | - |
dc.subject | decarboxylation | - |
dc.subject | herb | - |
dc.title | In planta study of photosynthesis and photorespiration using NADPH and NADH/NAD+ fluorescent protein sensors | - |
dc.type | Article | - |
dc.identifier.email | Lim, BL: bllim@hku.hk | - |
dc.identifier.authority | Lim, BL=rp00744 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1038/s41467-020-17056-0 | - |
dc.identifier.pmid | 32591540 | - |
dc.identifier.pmcid | PMC7320160 | - |
dc.identifier.scopus | eid_2-s2.0-85086844355 | - |
dc.identifier.hkuros | 313017 | - |
dc.identifier.volume | 11 | - |
dc.identifier.spage | article no. 3238 | - |
dc.identifier.epage | article no. 3238 | - |
dc.identifier.isi | WOS:000544946500003 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 2041-1723 | - |