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- Publisher Website: 10.1128/JCM.00936-20
- Scopus: eid_2-s2.0-85088611606
- PMID: 32482633
- WOS: WOS:000573919100024
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Article: Clinical Performance of the Luminex NxTAG CoV Extended Panel for SARS-CoV-2 Detection in Nasopharyngeal Specimens from COVID-19 Patients in Hong Kong
Title | Clinical Performance of the Luminex NxTAG CoV Extended Panel for SARS-CoV-2 Detection in Nasopharyngeal Specimens from COVID-19 Patients in Hong Kong |
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Authors | |
Keywords | COVID-19 Luminex NxTAG RT-PCR SARS-CoV-2 |
Issue Date | 2020 |
Publisher | American Society for Microbiology. The Journal's web site is located at http://jcm.asm.org/ |
Citation | Journal of Clinical Microbiology, 2020, v. 58 n. 8, p. article no. e00936-20 How to Cite? |
Abstract | In December 2019, the coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) was first reported in the Hubei province of China and later spread all over the world. There was an urgent need of a high-throughput molecular test for screening the COVID-19 patients in the community. The Luminex NxTAG CoV extended panel is a high-throughput FDA emergency use-authorized molecular diagnostic assay for SARS-CoV-2 detection. This system targets three genes (ORF1ab, N, and E genes) of SARS-CoV-2, the ORF1ab region of SARS-CoV, and the ORF5 region of MERS-CoV. In this study, we evaluated the diagnostic performance of this system with nasopharyngeal swab specimens of 214 suspected COVID-19 patients in Hong Kong. The results were compared with our routine COVID-19 reverse transcription-PCR (RT-PCR) protocol with a LightMix SarbecoV E-gene kit and an in-house RdRp/Hel RT-PCR assay. The NxTAG CoV extended panel demonstrated 97.8% sensitivity and 100% specificity to SARS-CoV-2 in nasopharyngeal specimens. On low-viral load specimens, the sensitivity of the NxTAG panel could still maintain at 85.71%. Strong agreement was observed between the NxTAG panel and the routine COVID-19 RT-PCR protocol (kappa value = 0.98). Overall, the E gene target of the NxTAG panel demonstrated the highest sensitivity among the three SARS-CoV-2 targets, while the N gene targets demonstrated the least. In conclusion, the NxTAG CoV extended panel is simple to use, and it has high diagnostic sensitivity and specificity to SARS-CoV-2 in nasopharyngeal specimens. We recommend this diagnostic system for high-throughput COVID-19 screening in the community. |
Persistent Identifier | http://hdl.handle.net/10722/285302 |
ISSN | 2023 Impact Factor: 6.1 2023 SCImago Journal Rankings: 1.653 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Chen, JHK | - |
dc.contributor.author | Yip, CCY | - |
dc.contributor.author | Chan, JFW | - |
dc.contributor.author | Poon, RWS | - |
dc.contributor.author | To, KKW | - |
dc.contributor.author | Chan, KH | - |
dc.contributor.author | Cheng, VCC | - |
dc.contributor.author | Yuen, KY | - |
dc.date.accessioned | 2020-08-18T03:52:12Z | - |
dc.date.available | 2020-08-18T03:52:12Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Journal of Clinical Microbiology, 2020, v. 58 n. 8, p. article no. e00936-20 | - |
dc.identifier.issn | 0095-1137 | - |
dc.identifier.uri | http://hdl.handle.net/10722/285302 | - |
dc.description.abstract | In December 2019, the coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) was first reported in the Hubei province of China and later spread all over the world. There was an urgent need of a high-throughput molecular test for screening the COVID-19 patients in the community. The Luminex NxTAG CoV extended panel is a high-throughput FDA emergency use-authorized molecular diagnostic assay for SARS-CoV-2 detection. This system targets three genes (ORF1ab, N, and E genes) of SARS-CoV-2, the ORF1ab region of SARS-CoV, and the ORF5 region of MERS-CoV. In this study, we evaluated the diagnostic performance of this system with nasopharyngeal swab specimens of 214 suspected COVID-19 patients in Hong Kong. The results were compared with our routine COVID-19 reverse transcription-PCR (RT-PCR) protocol with a LightMix SarbecoV E-gene kit and an in-house RdRp/Hel RT-PCR assay. The NxTAG CoV extended panel demonstrated 97.8% sensitivity and 100% specificity to SARS-CoV-2 in nasopharyngeal specimens. On low-viral load specimens, the sensitivity of the NxTAG panel could still maintain at 85.71%. Strong agreement was observed between the NxTAG panel and the routine COVID-19 RT-PCR protocol (kappa value = 0.98). Overall, the E gene target of the NxTAG panel demonstrated the highest sensitivity among the three SARS-CoV-2 targets, while the N gene targets demonstrated the least. In conclusion, the NxTAG CoV extended panel is simple to use, and it has high diagnostic sensitivity and specificity to SARS-CoV-2 in nasopharyngeal specimens. We recommend this diagnostic system for high-throughput COVID-19 screening in the community. | - |
dc.language | eng | - |
dc.publisher | American Society for Microbiology. The Journal's web site is located at http://jcm.asm.org/ | - |
dc.relation.ispartof | Journal of Clinical Microbiology | - |
dc.rights | Journal of Clinical Microbiology. Copyright © American Society for Microbiology. | - |
dc.subject | COVID-19 | - |
dc.subject | Luminex NxTAG | - |
dc.subject | RT-PCR | - |
dc.subject | SARS-CoV-2 | - |
dc.title | Clinical Performance of the Luminex NxTAG CoV Extended Panel for SARS-CoV-2 Detection in Nasopharyngeal Specimens from COVID-19 Patients in Hong Kong | - |
dc.type | Article | - |
dc.identifier.email | Chen, JHK: jonchk@hku.hk | - |
dc.identifier.email | Yip, CCY: yipcyril@hku.hk | - |
dc.identifier.email | Chan, JFW: jfwchan@hku.hk | - |
dc.identifier.email | Poon, RWS: rosana@hkucc.hku.hk | - |
dc.identifier.email | To, KKW: kelvinto@hku.hk | - |
dc.identifier.email | Chan, KH: chankh2@hkucc.hku.hk | - |
dc.identifier.email | Cheng, VCC: vcccheng@hkucc.hku.hk | - |
dc.identifier.email | Yuen, KY: kyyuen@hkucc.hku.hk | - |
dc.identifier.authority | Yip, CCY=rp01721 | - |
dc.identifier.authority | Chan, JFW=rp01736 | - |
dc.identifier.authority | To, KKW=rp01384 | - |
dc.identifier.authority | Chan, KH=rp01921 | - |
dc.identifier.authority | Yuen, KY=rp00366 | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1128/JCM.00936-20 | - |
dc.identifier.pmid | 32482633 | - |
dc.identifier.pmcid | PMC7383549 | - |
dc.identifier.scopus | eid_2-s2.0-85088611606 | - |
dc.identifier.hkuros | 312831 | - |
dc.identifier.volume | 58 | - |
dc.identifier.issue | 8 | - |
dc.identifier.spage | article no. e00936 | - |
dc.identifier.epage | 20 | - |
dc.identifier.isi | WOS:000573919100024 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0095-1137 | - |