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Article: Polyunsaturated linolenoyl‐CoA modulates ERF‐VII‐mediated hypoxia signaling in Arabidopsis

TitlePolyunsaturated linolenoyl‐CoA modulates ERF‐VII‐mediated hypoxia signaling in Arabidopsis
Authors
Keywordsfatty acid
gene expression
hypoxia
membrane
plasma
Issue Date2020
PublisherWiley-Blackwell Publishing Asia. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1744-7909
Citation
Journal of Intergrative Plant Biology, 2020, v. 62 n. 3, p. 330-348 How to Cite?
AbstractIn plants, submergence from flooding causes hypoxia, which impairs energy production and affects plant growth, productivity, and survival. In Arabidopsis, hypoxia induces nuclear localization of the group VII ethylene‐responsive transcription factor RELATED TO AP2.12 (RAP2.12), following its dissociation from the plasma membrane‐anchored ACYL‐COA BINDING PROTEIN1 (ACBP1) and ACBP2. Here, we show that polyunsaturated linolenoyl‐CoA (18:3‐CoA) regulates RAP2.12 release from the plasma membrane. Submergence caused a significant increase in 18:3‐CoA, but a significant decrease in 18:0‐, 18:1‐, and 18:2‐CoA. Application of 18:3‐CoA promoted nuclear accumulation of the green fluorescent protein (GFP) fusions RAP2.12‐GFP, HYPOXIA‐RESPONSIVE ERF1‐GFP, and RAP2.3‐GFP, and enhanced transcript levels of hypoxia‐responsive genes. Plants with decreased ACBP1 and ACBP2 (acbp1 ACBP2‐RNAi, produced by ACBP2 RNA interference in the acbp1 mutant) had reduced tolerance to hypoxia and impaired 18:3‐CoA‐induced expression of hypoxia‐related genes. In knockout mutants and overexpression lines of LONG‐CHAIN ACYL‐COA SYNTHASE2 (LACS2) and FATTY ACID DESATURASE 3 (FAD3), the acyl‐CoA pool size and 18:3‐CoA levels were closely related to ERF‐VII‐mediated signaling and hypoxia tolerance. These findings demonstrate that polyunsaturation of long‐chain acyl‐CoAs functions as important mechanism in the regulation of plant hypoxia signaling, by modulating ACBP–ERF‐VII dynamics.
Persistent Identifierhttp://hdl.handle.net/10722/284705
ISSN
2019 Impact Factor: 4.885
2015 SCImago Journal Rankings: 1.674

 

DC FieldValueLanguage
dc.contributor.authorZHOU, Y-
dc.contributor.authorTan, WJ-
dc.contributor.authorXie, LJ-
dc.contributor.authorQi, H-
dc.contributor.authorYang, YC-
dc.contributor.authorHuang, LP-
dc.contributor.authorLai, YX-
dc.contributor.authorTan, YF-
dc.contributor.authorZhou, DM-
dc.contributor.authorYu, LJ-
dc.contributor.authorChen, QF-
dc.contributor.authorChye, ML-
dc.contributor.authorXiao, S-
dc.date.accessioned2020-08-07T09:01:33Z-
dc.date.available2020-08-07T09:01:33Z-
dc.date.issued2020-
dc.identifier.citationJournal of Intergrative Plant Biology, 2020, v. 62 n. 3, p. 330-348-
dc.identifier.issn1672-9072-
dc.identifier.urihttp://hdl.handle.net/10722/284705-
dc.description.abstractIn plants, submergence from flooding causes hypoxia, which impairs energy production and affects plant growth, productivity, and survival. In Arabidopsis, hypoxia induces nuclear localization of the group VII ethylene‐responsive transcription factor RELATED TO AP2.12 (RAP2.12), following its dissociation from the plasma membrane‐anchored ACYL‐COA BINDING PROTEIN1 (ACBP1) and ACBP2. Here, we show that polyunsaturated linolenoyl‐CoA (18:3‐CoA) regulates RAP2.12 release from the plasma membrane. Submergence caused a significant increase in 18:3‐CoA, but a significant decrease in 18:0‐, 18:1‐, and 18:2‐CoA. Application of 18:3‐CoA promoted nuclear accumulation of the green fluorescent protein (GFP) fusions RAP2.12‐GFP, HYPOXIA‐RESPONSIVE ERF1‐GFP, and RAP2.3‐GFP, and enhanced transcript levels of hypoxia‐responsive genes. Plants with decreased ACBP1 and ACBP2 (acbp1 ACBP2‐RNAi, produced by ACBP2 RNA interference in the acbp1 mutant) had reduced tolerance to hypoxia and impaired 18:3‐CoA‐induced expression of hypoxia‐related genes. In knockout mutants and overexpression lines of LONG‐CHAIN ACYL‐COA SYNTHASE2 (LACS2) and FATTY ACID DESATURASE 3 (FAD3), the acyl‐CoA pool size and 18:3‐CoA levels were closely related to ERF‐VII‐mediated signaling and hypoxia tolerance. These findings demonstrate that polyunsaturation of long‐chain acyl‐CoAs functions as important mechanism in the regulation of plant hypoxia signaling, by modulating ACBP–ERF‐VII dynamics.-
dc.languageeng-
dc.publisherWiley-Blackwell Publishing Asia. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1744-7909-
dc.relation.ispartofJournal of Intergrative Plant Biology-
dc.rightsPreprint This is the pre-peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. Postprint This is the peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.-
dc.subjectfatty acid-
dc.subjectgene expression-
dc.subjecthypoxia-
dc.subjectmembrane-
dc.subjectplasma-
dc.titlePolyunsaturated linolenoyl‐CoA modulates ERF‐VII‐mediated hypoxia signaling in Arabidopsis-
dc.typeArticle-
dc.identifier.emailChye, ML: mlchye@hku.hk-
dc.identifier.authorityChye, ML=rp00687-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/jipb.12875-
dc.identifier.pmid31595698-
dc.identifier.scopuseid_2-s2.0-85078682573-
dc.identifier.hkuros311908-
dc.identifier.volume62-
dc.identifier.issue3-
dc.identifier.spage330-
dc.identifier.epage348-
dc.publisher.placeAustralia-

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