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Article: Gene expression changes associated with malignant transformation of oral potentially malignant disorders

TitleGene expression changes associated with malignant transformation of oral potentially malignant disorders
Authors
Keywordsmalignant
transformation
oral potentially malignant disorders
oral squamous cell carcinoma
RNA‐Sequencing
Issue Date2020
PublisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1600-0714
Citation
Journal of Oral Pathology & Medicine, 2020, Epub 2020-08-02 How to Cite?
AbstractBackground: A large number of oral squamous cell carcinomas (OSCCs) are believed to be preceded by oral potentially malignant disorders (OPMD) that have an increased likelihood of malignant transformation compared to clinically normal mucosa. This study was performed to identify differentially expressed genes between OPMDs that underwent malignant transformation (MT) and those that did not, termed “non‐transforming” (NT) cases. Methods: Total RNA was extracted from formalin‐fixed paraffin‐embedded tissue biopsies of 20 OPMD cases with known clinical outcomes (10 MT vs. 10 NT). Samples were assessed for quantity, quality and integrity of RNA prior to sequencing. Analysis for differential gene expression between MT and NT was performed using statistical packages in R. Genes were considered to be significantly differentially expressed if the False Discovery Rate corrected P‐value was < 0.05. Results: RNA yield was variable but RNA purity was good (A260/A280 > 1.90). Analysis of RNA‐Sequencing outputs revealed 41 genes (34 protein‐coding; 7 non‐coding) that were significantly differentially expressed between MT and NT cases. The log2 fold change for the statistically significant differentially expressed genes ranged from −2.63 to 2.48, with 23 protein‐coding genes being downregulated and 11 protein‐coding genes being upregulated in MT cases compared to NT cases. Conclusion: Several candidate genes that may play a role in malignant transformation of OPMD have been identified. Experiments to validate these candidates are underway. It is anticipated that this work will contribute to better understanding of the etiopathogenesis of OPMD and development of novel biomarkers.
Persistent Identifierhttp://hdl.handle.net/10722/284523
ISSN
2019 Impact Factor: 2.495
2015 SCImago Journal Rankings: 0.731

 

DC FieldValueLanguage
dc.contributor.authorSathasivam, HP-
dc.contributor.authorCasement, J-
dc.contributor.authorBates, T-
dc.contributor.authorSloan, P-
dc.contributor.authorThomson, P-
dc.contributor.authorRobinson, M-
dc.contributor.authorKist, R-
dc.date.accessioned2020-08-07T08:58:52Z-
dc.date.available2020-08-07T08:58:52Z-
dc.date.issued2020-
dc.identifier.citationJournal of Oral Pathology & Medicine, 2020, Epub 2020-08-02-
dc.identifier.issn0904-2512-
dc.identifier.urihttp://hdl.handle.net/10722/284523-
dc.description.abstractBackground: A large number of oral squamous cell carcinomas (OSCCs) are believed to be preceded by oral potentially malignant disorders (OPMD) that have an increased likelihood of malignant transformation compared to clinically normal mucosa. This study was performed to identify differentially expressed genes between OPMDs that underwent malignant transformation (MT) and those that did not, termed “non‐transforming” (NT) cases. Methods: Total RNA was extracted from formalin‐fixed paraffin‐embedded tissue biopsies of 20 OPMD cases with known clinical outcomes (10 MT vs. 10 NT). Samples were assessed for quantity, quality and integrity of RNA prior to sequencing. Analysis for differential gene expression between MT and NT was performed using statistical packages in R. Genes were considered to be significantly differentially expressed if the False Discovery Rate corrected P‐value was < 0.05. Results: RNA yield was variable but RNA purity was good (A260/A280 > 1.90). Analysis of RNA‐Sequencing outputs revealed 41 genes (34 protein‐coding; 7 non‐coding) that were significantly differentially expressed between MT and NT cases. The log2 fold change for the statistically significant differentially expressed genes ranged from −2.63 to 2.48, with 23 protein‐coding genes being downregulated and 11 protein‐coding genes being upregulated in MT cases compared to NT cases. Conclusion: Several candidate genes that may play a role in malignant transformation of OPMD have been identified. Experiments to validate these candidates are underway. It is anticipated that this work will contribute to better understanding of the etiopathogenesis of OPMD and development of novel biomarkers.-
dc.languageeng-
dc.publisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1600-0714-
dc.relation.ispartofJournal of Oral Pathology & Medicine-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectmalignant-
dc.subjecttransformation-
dc.subjectoral potentially malignant disorders-
dc.subjectoral squamous cell carcinoma-
dc.subjectRNA‐Sequencing-
dc.titleGene expression changes associated with malignant transformation of oral potentially malignant disorders-
dc.typeArticle-
dc.identifier.emailThomson, P: thomsonp@hku.hk-
dc.identifier.authorityThomson, P=rp02327-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1111/jop.13090-
dc.identifier.pmid32740996-
dc.identifier.scopuseid_2-s2.0-85090066695-
dc.identifier.hkuros312461-
dc.identifier.volumeEpub 2020-08-02-
dc.publisher.placeDenmark-

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