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Postgraduate Thesis: A comparative study of some possible mechanisms governing the intraluminal and intravascular release of 5-HT from the rabbitjejunum
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TitleA comparative study of some possible mechanisms governing the intraluminal and intravascular release of 5-HT from the rabbitjejunum
 
AuthorsWong, Chi-yan, Joseph
黃智仁
 
Issue Date1973
 
PublisherThe University of Hong Kong (Pokfulam, Hong Kong)
 
Abstract(Uncorrected OCR) Abstract Abstract of thesis entitled "A comparative study of some possible mechanisms governing the intraluminal and intravascular release of 5-HT from the rabbit jejunum" submitted by WONG Joseph Chi-yan for the degree of Doctor of Philosophy (Pharmacology) at the University of Hong Kong in April 1973. Experiments were carried out on rabbit jejunal segments in. vivo and in vitro under conditions of intraluminal and intravascular perfusion. 5-HT was released into the effluent of both intraluminal- and intravascular perfusate during perfusion with Krebs solution under quiescent conditions. Enhanced 5-HT release was observed during active peristalsis elicited by increasing the intraluminalpressure during intraluminal perfusion. The addition of hydrochloric acid in physiological amounts to give pH values of 7.2, 6, 5 and 4 in Krebs solution revealed a progressive increase in 5-HT release as the pH fell, during intraluminal perfusion. Enhanced 5-HT release was also observed during intraluminal perfusions with 0.3 M and 0.6 M sucrose solutions; 0.6 M glucose solution and 7.5% egg albumin in Krebs solution. During intraluminal perfusion, Sennosides A & BlO ~g/ml., Nicotine 1 ~g/ml. and Morphine 10 ~g/ml. in Krebs solution, all enhanced 5-HT release; but not 1% Monosodium L-glutamate. Monoamine oxidase inhibitor, Nialamide, in a concentration of 100 ~g/ml. prevented . the destruction of both endogenous and exogenous 5-HT. Nicotine 1 ~g/ml. and Morphine 10 ~g/ml. did not enhance 5-HT release during intravascular perfusion. From the above observations, it is concluded that, in rabbits, 5-HT is involved in the normal physiological functions of digestion, absorption and motility. It possibly functions as a local hormone in regulating these processes. The intravascular release of 5-HT demonstrated in these experiments may not have any direct physiologic~l significance. It is not appropriate to compare the two perfusion methods in terms of the net amount of released 5-HT. From histological observations, it is suspected that the results of intraluminal perfusion are complicated by the shedding off of intestinal epithelial cells, into the perfusing fluid. Further investigations are needed to evaluate the reliability of this method in the study of the phenomenon of 115-HT release II under such experimental conditions, and the interpretation which can be put upon the results obtained. ii
 
DegreeDoctor of Philosophy
 
SubjectSerotonin.
Pharmacology.
 
Dept/ProgramPharmacology
 
DC FieldValue
dc.contributor.authorWong, Chi-yan, Joseph
 
dc.contributor.author黃智仁
 
dc.date.issued1973
 
dc.description.abstract(Uncorrected OCR) Abstract Abstract of thesis entitled "A comparative study of some possible mechanisms governing the intraluminal and intravascular release of 5-HT from the rabbit jejunum" submitted by WONG Joseph Chi-yan for the degree of Doctor of Philosophy (Pharmacology) at the University of Hong Kong in April 1973. Experiments were carried out on rabbit jejunal segments in. vivo and in vitro under conditions of intraluminal and intravascular perfusion. 5-HT was released into the effluent of both intraluminal- and intravascular perfusate during perfusion with Krebs solution under quiescent conditions. Enhanced 5-HT release was observed during active peristalsis elicited by increasing the intraluminalpressure during intraluminal perfusion. The addition of hydrochloric acid in physiological amounts to give pH values of 7.2, 6, 5 and 4 in Krebs solution revealed a progressive increase in 5-HT release as the pH fell, during intraluminal perfusion. Enhanced 5-HT release was also observed during intraluminal perfusions with 0.3 M and 0.6 M sucrose solutions; 0.6 M glucose solution and 7.5% egg albumin in Krebs solution. During intraluminal perfusion, Sennosides A & BlO ~g/ml., Nicotine 1 ~g/ml. and Morphine 10 ~g/ml. in Krebs solution, all enhanced 5-HT release; but not 1% Monosodium L-glutamate. Monoamine oxidase inhibitor, Nialamide, in a concentration of 100 ~g/ml. prevented . the destruction of both endogenous and exogenous 5-HT. Nicotine 1 ~g/ml. and Morphine 10 ~g/ml. did not enhance 5-HT release during intravascular perfusion. From the above observations, it is concluded that, in rabbits, 5-HT is involved in the normal physiological functions of digestion, absorption and motility. It possibly functions as a local hormone in regulating these processes. The intravascular release of 5-HT demonstrated in these experiments may not have any direct physiologic~l significance. It is not appropriate to compare the two perfusion methods in terms of the net amount of released 5-HT. From histological observations, it is suspected that the results of intraluminal perfusion are complicated by the shedding off of intestinal epithelial cells, into the perfusing fluid. Further investigations are needed to evaluate the reliability of this method in the study of the phenomenon of 115-HT release II under such experimental conditions, and the interpretation which can be put upon the results obtained. ii
 
dc.description.natureabstract
 
dc.description.naturetoc
 
dc.description.thesisdisciplinePharmacology
 
dc.description.thesisleveldoctoral
 
dc.description.thesisnameDoctor of Philosophy
 
dc.identifier.hkulb1227510
 
dc.languageeng
 
dc.publisherThe University of Hong Kong (Pokfulam, Hong Kong)
 
dc.relation.ispartofHKU Theses Online (HKUTO)
 
dc.rightsThe author retains all proprietary rights, (such as patent rights) and the right to use in future works.
 
dc.source.urihttp://hub.hku.hk/bib/B12275104
 
dc.subject.lcshSerotonin.
 
dc.subject.lcshPharmacology.
 
dc.titleA comparative study of some possible mechanisms governing the intraluminal and intravascular release of 5-HT from the rabbitjejunum
 
dc.typePG_Thesis
 
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<item><contributor.author>Wong, Chi-yan, Joseph</contributor.author>
<contributor.author>&#40643;&#26234;&#20161;</contributor.author>
<date.issued>1973</date.issued>
<description.abstract>(Uncorrected OCR) 
Abstract 

Abstract of thesis entitled &quot;A comparative study of some possible mechanisms governing the intraluminal and intravascular release of 5-HT from the rabbit jejunum&quot; submitted by WONG Joseph Chi-yan 

for the degree of Doctor of Philosophy (Pharmacology) at the University of Hong Kong in April 1973. 

Experiments were carried out on rabbit jejunal segments in. vivo and in vitro under conditions of intraluminal and intravascular perfusion. 5-HT was released into the effluent of both intraluminal- and intravascular perfusate during perfusion with Krebs solution under quiescent conditions. Enhanced 5-HT release was observed during active peristalsis elicited by increasing the intraluminalpressure during intraluminal perfusion. The addition of hydrochloric acid in physiological amounts 

to give pH values of 7.2, 6, 5 and 4 in Krebs solution revealed a progressive increase in 5-HT release as the pH fell, during intraluminal perfusion. Enhanced 5-HT release was also observed during intraluminal perfusions with 0.3 M and 0.6 M sucrose solutions; 0.6 M glucose solution and 7.5% egg albumin in Krebs solution. 

During intraluminal perfusion, Sennosides A &amp; 

BlO ~g/ml., Nicotine 1 ~g/ml. and Morphine 10 ~g/ml. in Krebs solution, all enhanced 5-HT release; but not 1% Monosodium L-glutamate. Monoamine oxidase inhibitor, 

Nialamide, in a concentration of 100 ~g/ml. prevented . the destruction of both endogenous and exogenous 5-HT. 

Nicotine 1 ~g/ml. and Morphine 10 ~g/ml. did not enhance 5-HT release during intravascular perfusion. 

From the above observations, it is concluded that, in rabbits, 5-HT is involved in the normal physiological functions of digestion, absorption and motility. It possibly functions as a local hormone in regulating these processes. The intravascular release of 5-HT demonstrated in these experiments may not have any direct physiologic~l significance. 

It is not appropriate to compare the two perfusion methods in terms of the net amount of released 5-HT. From histological observations, it is suspected that the results of intraluminal perfusion are complicated by the shedding off of intestinal epithelial cells, into the perfusing fluid. Further investigations are needed to evaluate the reliability of this method in the study of the phenomenon of 115-HT release II under such experimental conditions, and the interpretation which can be put upon the results obtained. 

ii</description.abstract>
<language>eng</language>
<publisher>The University of Hong Kong (Pokfulam, Hong Kong)</publisher>
<relation.ispartof>HKU Theses Online (HKUTO)</relation.ispartof>
<rights>The author retains all proprietary rights, (such as patent rights) and the right to use in future works.</rights>
<source.uri>http://hub.hku.hk/bib/B12275104</source.uri>
<subject.lcsh>Serotonin.</subject.lcsh>
<subject.lcsh>Pharmacology.</subject.lcsh>
<title>A comparative study of some possible mechanisms governing the intraluminal and intravascular release of 5-HT from the rabbitjejunum</title>
<type>PG_Thesis</type>
<identifier.hkul>b1227510</identifier.hkul>
<description.thesisname>Doctor of Philosophy</description.thesisname>
<description.thesislevel>doctoral</description.thesislevel>
<description.thesisdiscipline>Pharmacology</description.thesisdiscipline>
<description.nature>abstract</description.nature>
<description.nature>toc</description.nature>
<bitstream.url>http://hub.hku.hk/bitstream/10722/28320/5/Abstract.pdf</bitstream.url>
<bitstream.url>http://hub.hku.hk/bitstream/10722/28320/6/Table%20of%20Contents.pdf</bitstream.url>
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