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- Publisher Website: 10.1128/AAC.02529-19
- Scopus: eid_2-s2.0-85085266172
- PMID: 32205352
- WOS: WOS:000535946300053
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Article: Metronidazole-Treated Porphyromonas gingivalis Persisters Invade Human Gingival Epithelial Cells and Perturb Innate Responses
Title | Metronidazole-Treated Porphyromonas gingivalis Persisters Invade Human Gingival Epithelial Cells and Perturb Innate Responses |
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Authors | |
Keywords | metronidazole Porphyromonas gingivalis persisters human gingival epithelial cell innate response |
Issue Date | 2020 |
Publisher | American Society for Microbiology. The Journal's web site is located at http://aac.asm.org/ |
Citation | Antimicrobial Agents and Chemotherapy, 2020, v. 64 n. 6, p. article no. e02529-19 How to Cite? |
Abstract | Periodontitis as a biofilm-associated inflammatory disease is highly prevalent worldwide. It severely affects oral health and yet closely links to systemic diseases like diabetes and cardiovascular disease. Porphyromonas gingivalis as a “keystone” periodontopathogen drives the shift of microbe-host symbiosis to dysbiosis and critically contributes to the pathogenesis of periodontitis. Persisters represent a tiny subset of biofilm-associated microbes highly tolerant to lethal treatment of antimicrobials, and, notably, metronidazole-tolerant P. gingivalis persisters have recently been identified by our group. This study further explored the interactive profiles of metronidazole-treated P. gingivalis persisters (M-PgPs) with human gingival epithelial cells (HGECs). P. gingivalis cells (ATCC 33277) at stationary phase were treated with a lethal dosage of metronidazole (100 μg/ml, 6 h) for generating M-PgPs. The interaction of M-PgPs with HGECs was assessed by microscopy, flow cytometry, cytokine profiling, and quantitative PCR (qPCR). We demonstrated that the overall morphology and ultracellular structure of M-PgPs remained unchanged. Importantly, M-PgPs maintained the capabilities to adhere to and invade HGECs. Moreover, M-PgPs significantly suppressed proinflammatory cytokine expression in HGECs at a level comparable to that seen with the untreated P. gingivalis cells, through the thermosensitive components. The present report reveals that P. gingivalis persisters induced by lethal treatment of antibiotics were able to maintain their capabilities to adhere to and invade human gingival epithelial cells and to perturb the innate host responses. Novel strategies and approaches need to be developed for tackling P. gingivalis and favorably modulating the dysregulated immunoinflammatory responses for oral/periodontal health and general well-being. |
Persistent Identifier | http://hdl.handle.net/10722/282871 |
ISSN | 2023 Impact Factor: 4.1 2023 SCImago Journal Rankings: 1.357 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | WANG, C | - |
dc.contributor.author | Cheng, T | - |
dc.contributor.author | Li, X | - |
dc.contributor.author | Jin, L | - |
dc.date.accessioned | 2020-06-05T06:22:31Z | - |
dc.date.available | 2020-06-05T06:22:31Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Antimicrobial Agents and Chemotherapy, 2020, v. 64 n. 6, p. article no. e02529-19 | - |
dc.identifier.issn | 0066-4804 | - |
dc.identifier.uri | http://hdl.handle.net/10722/282871 | - |
dc.description.abstract | Periodontitis as a biofilm-associated inflammatory disease is highly prevalent worldwide. It severely affects oral health and yet closely links to systemic diseases like diabetes and cardiovascular disease. Porphyromonas gingivalis as a “keystone” periodontopathogen drives the shift of microbe-host symbiosis to dysbiosis and critically contributes to the pathogenesis of periodontitis. Persisters represent a tiny subset of biofilm-associated microbes highly tolerant to lethal treatment of antimicrobials, and, notably, metronidazole-tolerant P. gingivalis persisters have recently been identified by our group. This study further explored the interactive profiles of metronidazole-treated P. gingivalis persisters (M-PgPs) with human gingival epithelial cells (HGECs). P. gingivalis cells (ATCC 33277) at stationary phase were treated with a lethal dosage of metronidazole (100 μg/ml, 6 h) for generating M-PgPs. The interaction of M-PgPs with HGECs was assessed by microscopy, flow cytometry, cytokine profiling, and quantitative PCR (qPCR). We demonstrated that the overall morphology and ultracellular structure of M-PgPs remained unchanged. Importantly, M-PgPs maintained the capabilities to adhere to and invade HGECs. Moreover, M-PgPs significantly suppressed proinflammatory cytokine expression in HGECs at a level comparable to that seen with the untreated P. gingivalis cells, through the thermosensitive components. The present report reveals that P. gingivalis persisters induced by lethal treatment of antibiotics were able to maintain their capabilities to adhere to and invade human gingival epithelial cells and to perturb the innate host responses. Novel strategies and approaches need to be developed for tackling P. gingivalis and favorably modulating the dysregulated immunoinflammatory responses for oral/periodontal health and general well-being. | - |
dc.language | eng | - |
dc.publisher | American Society for Microbiology. The Journal's web site is located at http://aac.asm.org/ | - |
dc.relation.ispartof | Antimicrobial Agents and Chemotherapy | - |
dc.rights | Antimicrobial Agents and Chemotherapy. Copyright © American Society for Microbiology. | - |
dc.subject | metronidazole | - |
dc.subject | Porphyromonas gingivalis | - |
dc.subject | persisters | - |
dc.subject | human gingival epithelial cell | - |
dc.subject | innate response | - |
dc.title | Metronidazole-Treated Porphyromonas gingivalis Persisters Invade Human Gingival Epithelial Cells and Perturb Innate Responses | - |
dc.type | Article | - |
dc.identifier.email | Cheng, T: chengtfc@hku.hk | - |
dc.identifier.email | Li, X: llx815@hku.hk | - |
dc.identifier.email | Jin, L: ljjin@hkucc.hku.hk | - |
dc.identifier.authority | Li, X=rp02494 | - |
dc.identifier.authority | Jin, L=rp00028 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1128/AAC.02529-19 | - |
dc.identifier.pmid | 32205352 | - |
dc.identifier.scopus | eid_2-s2.0-85085266172 | - |
dc.identifier.hkuros | 310125 | - |
dc.identifier.volume | 64 | - |
dc.identifier.issue | 6 | - |
dc.identifier.spage | article no. e02529 | - |
dc.identifier.epage | 19 | - |
dc.identifier.isi | WOS:000535946300053 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0066-4804 | - |