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Article: Small RNA profiling of piRNAs in colorectal cancer identifies consistent overexpression of piR-24000 that correlates clinically with an aggressive disease phenotype

TitleSmall RNA profiling of piRNAs in colorectal cancer identifies consistent overexpression of piR-24000 that correlates clinically with an aggressive disease phenotype
Authors
KeywordspiRNA
colorectal cancer
prognosis
piR-24000
non-coding RNA
Issue Date2020
PublisherMDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/cancers/
Citation
Cancers, 2020, v. 12 n. 1, article no. 188 How to Cite?
AbstractPiwi-interacting RNAs (piRNAs) represent a novel class of small non-coding RNAs (ncRNAs) that have been shown to have a deregulated expression in several cancers, although their clinical significance in colorectal cancer (CRC) remains unclear. With an aim of delineating the piRNA distribution in CRC, we conducted a systematic discovery and validation of piRNAs within two clinical cohorts. In the discovery phase, we profiled tumor and adjacent normal tissues from 18 CRC patients by deep sequencing and identified a global piRNA downregulation in CRC. Moreover, we identified piR-24000 as an unexplored piRNA that was significantly overexpressed in CRC. Using qPCR, we validated the overexpression of piR-24000 in 87 CRC patients. Additionally, we identified a significant association between a high expression of piR-24000 and an aggressive CRC phenotype including poor differentiation, presence of distant metastases, and a higher stage. Lastly, ROC analysis demonstrated a strong diagnostic power of piR-24000 in discriminating CRC patients from normal subjects. Taken together, this study provides one of the earliest large-scale reports of the global distribution of piRNAs in CRC. In addition, piR-24000 was identified as a likely oncogene in CRC that can serve as a biomarker or a therapeutic target.
Persistent Identifierhttp://hdl.handle.net/10722/280319
ISSN
2018 Impact Factor: 6.162
2015 SCImago Journal Rankings: 1.630

 

DC FieldValueLanguage
dc.contributor.authorIyer, DN-
dc.contributor.authorWan, TMH-
dc.contributor.authorMan, JHW-
dc.contributor.authorSin, RWY-
dc.contributor.authorLi, X-
dc.contributor.authorLo, OSH-
dc.contributor.authorFoo, DCC-
dc.contributor.authorPang, RWC-
dc.contributor.authorLaw, WL-
dc.contributor.authorNg, L-
dc.date.accessioned2020-02-07T07:39:27Z-
dc.date.available2020-02-07T07:39:27Z-
dc.date.issued2020-
dc.identifier.citationCancers, 2020, v. 12 n. 1, article no. 188-
dc.identifier.issn2072-6694-
dc.identifier.urihttp://hdl.handle.net/10722/280319-
dc.description.abstractPiwi-interacting RNAs (piRNAs) represent a novel class of small non-coding RNAs (ncRNAs) that have been shown to have a deregulated expression in several cancers, although their clinical significance in colorectal cancer (CRC) remains unclear. With an aim of delineating the piRNA distribution in CRC, we conducted a systematic discovery and validation of piRNAs within two clinical cohorts. In the discovery phase, we profiled tumor and adjacent normal tissues from 18 CRC patients by deep sequencing and identified a global piRNA downregulation in CRC. Moreover, we identified piR-24000 as an unexplored piRNA that was significantly overexpressed in CRC. Using qPCR, we validated the overexpression of piR-24000 in 87 CRC patients. Additionally, we identified a significant association between a high expression of piR-24000 and an aggressive CRC phenotype including poor differentiation, presence of distant metastases, and a higher stage. Lastly, ROC analysis demonstrated a strong diagnostic power of piR-24000 in discriminating CRC patients from normal subjects. Taken together, this study provides one of the earliest large-scale reports of the global distribution of piRNAs in CRC. In addition, piR-24000 was identified as a likely oncogene in CRC that can serve as a biomarker or a therapeutic target.-
dc.languageeng-
dc.publisherMDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/cancers/-
dc.relation.ispartofCancers-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectpiRNA-
dc.subjectcolorectal cancer-
dc.subjectprognosis-
dc.subjectpiR-24000-
dc.subjectnon-coding RNA-
dc.titleSmall RNA profiling of piRNAs in colorectal cancer identifies consistent overexpression of piR-24000 that correlates clinically with an aggressive disease phenotype-
dc.typeArticle-
dc.identifier.emailIyer, DN: deeiyer@hku.hk-
dc.identifier.emailWan, TMH: tmhwan@hku.hk-
dc.identifier.emailSin, RWY: wysin17@HKUCC-COM.hku.hk-
dc.identifier.emailLi, X: daisyxue@hku.hk-
dc.identifier.emailLo, OSH: oswens@hku.hk-
dc.identifier.emailFoo, DCC: ccfoo@hku.hk-
dc.identifier.emailLaw, WL: lawwl@hkucc.hku.hk-
dc.identifier.emailNg, L: luing@hku.hk-
dc.identifier.authorityFoo, DCC=rp01899-
dc.identifier.authorityLaw, WL=rp00436-
dc.identifier.authorityNg, L=rp02207-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3390/cancers12010188-
dc.identifier.scopuseid_2-s2.0-85078234318-
dc.identifier.hkuros309135-
dc.identifier.volume12-
dc.identifier.issue1-
dc.identifier.spagearticle no. 188-
dc.identifier.epagearticle no. 188-
dc.publisher.placeSwitzerland-

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