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Article: Pollination drop proteome and reproductive organ transcriptome comparison in Gnetum reveals entomophilous adaptation

TitlePollination drop proteome and reproductive organ transcriptome comparison in Gnetum reveals entomophilous adaptation
Authors
Keywordsinsect pollination
label-free quantitative sequencing
pollination drops
proteome
transcriptome;
Issue Date2019
PublisherMDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/genes
Citation
Genes, 2019, v. 10 n. 10, article no. 800 How to Cite?
AbstractGnetum possesses morphologically bisexual but functionally unisexual reproductive structures that exude sugary pollination drops to attract insects. Previous studies have revealed that the arborescent species (G. gnemon L.) and the lianoid species (G. luofuense C.Y.Cheng) possess different pollination syndromes. This study compared the proteome in the pollination drops of these two species using label-free quantitative techniques. The transcriptomes of fertile reproductive units (FRUs) and sterile reproductive units (SRUs) for each species were furthermore compared using Illumina Hiseq sequencing, and integrated proteomic and transcriptomic analyses were subsequently performed. Our results show that the differentially expressed proteins between FRUs and SRUs were involved in carbohydrate metabolism, the biosynthesis of amino acids and ovule defense. In addition, the differentially expressed genes between the FRUs and SRUs (e.g., MADS-box genes) were engaged in reproductive development and the formation of pollination drops. The integrated protein-transcript analyses revealed that FRUs and their exudates were relatively conservative while the SRUs and their exudates were more diverse, probably functioning as pollinator attractants. The evolution of reproductive organs appears to be synchronized with changes in the pollination drop proteome of Gnetum, suggesting that insect-pollinated adaptations are not restricted to angiosperms but also occur in gymnosperms.
Persistent Identifierhttp://hdl.handle.net/10722/280131
ISSN
2017 Impact Factor: 3.191
2015 SCImago Journal Rankings: 1.782
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHou, C-
dc.contributor.authorSaunders, RMK-
dc.contributor.authorDeng, N-
dc.contributor.authorWan, T-
dc.contributor.authorSu, Y-
dc.date.accessioned2020-01-06T02:01:29Z-
dc.date.available2020-01-06T02:01:29Z-
dc.date.issued2019-
dc.identifier.citationGenes, 2019, v. 10 n. 10, article no. 800-
dc.identifier.issn2073-4425-
dc.identifier.urihttp://hdl.handle.net/10722/280131-
dc.description.abstractGnetum possesses morphologically bisexual but functionally unisexual reproductive structures that exude sugary pollination drops to attract insects. Previous studies have revealed that the arborescent species (G. gnemon L.) and the lianoid species (G. luofuense C.Y.Cheng) possess different pollination syndromes. This study compared the proteome in the pollination drops of these two species using label-free quantitative techniques. The transcriptomes of fertile reproductive units (FRUs) and sterile reproductive units (SRUs) for each species were furthermore compared using Illumina Hiseq sequencing, and integrated proteomic and transcriptomic analyses were subsequently performed. Our results show that the differentially expressed proteins between FRUs and SRUs were involved in carbohydrate metabolism, the biosynthesis of amino acids and ovule defense. In addition, the differentially expressed genes between the FRUs and SRUs (e.g., MADS-box genes) were engaged in reproductive development and the formation of pollination drops. The integrated protein-transcript analyses revealed that FRUs and their exudates were relatively conservative while the SRUs and their exudates were more diverse, probably functioning as pollinator attractants. The evolution of reproductive organs appears to be synchronized with changes in the pollination drop proteome of Gnetum, suggesting that insect-pollinated adaptations are not restricted to angiosperms but also occur in gymnosperms.-
dc.languageeng-
dc.publisherMDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/genes-
dc.relation.ispartofGenes-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectinsect pollination-
dc.subjectlabel-free quantitative sequencing-
dc.subjectpollination drops-
dc.subjectproteome-
dc.subjecttranscriptome;-
dc.titlePollination drop proteome and reproductive organ transcriptome comparison in Gnetum reveals entomophilous adaptation-
dc.typeArticle-
dc.identifier.emailSaunders, RMK: saunders@hku.hk-
dc.identifier.authoritySaunders, RMK=rp00774-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3390/genes10100800-
dc.identifier.scopuseid_2-s2.0-85073442799-
dc.identifier.hkuros308905-
dc.identifier.volume10-
dc.identifier.issue10-
dc.identifier.spagearticle no. 800-
dc.identifier.epagearticle no. 800-
dc.identifier.isiWOS:000498397100071-
dc.publisher.placeSwitzerland-

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