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Article: Comparative transcriptomics analysis of Brassica napus L. during seed maturation reveals dynamic changes in gene expression between embryos and seed coats and distinct expression profiles of acyl-CoA-binding proteins for lipid accumulation

TitleComparative transcriptomics analysis of Brassica napus L. during seed maturation reveals dynamic changes in gene expression between embryos and seed coats and distinct expression profiles of acyl-CoA-binding proteins for lipid accumulation
Authors
KeywordsBrassica napus
ACBP
Embryos
Fatty acid
Oilseed rape
Issue Date2019
PublisherOxford University Press. The Journal's web site is located at http://pcp.oxfordjournals.org/
Citation
Plant and Cell Physiology, 2019, v. 60 n. 12, p. 2812-2825 How to Cite?
AbstractProduction of vegetable oils is a vital agricultural resource and oilseed rape (Brassica napus) is the third most important oil crop globally. Although the regulation of lipid biosynthesis in oilseeds is still not fully defined, the acyl-CoA-binding proteins (ACBPs) have been reported to be involved in such metabolism, including oil accumulation, in several plant species. In this study, progressive changes in gene expression in embryos and seed coats at different stages of seed development were comprehensively investigated by transcriptomic analyses in B. napus, revealing dynamic changes in the expression of genes involved in lipid biosynthesis. We show that genes encoding BnACBP proteins show distinct changes in expression at different developmental stages of seed development and show markedly different expression between embryos and seed coats. Both isoforms of the ankyrin-repeat BnACBP2 increased during the oil accumulation period of embryo development. By contrast, the expression of the three most abundant isoforms of the small molecular mass BnACBP6 in embryos showed progressive reduction, despite having the highest overall expression level. In seed coats, BnACBP3, BnACBP4 and BnACBP5 expression remained constant during development, whereas the two major isoforms of BnACBP6 increased, contrasting with the data from embryos. We conclude that genes related to fatty acid and triacylglycerol biosynthesis showing dynamic expression changes may regulate the lipid distribution in embryos and seed coats of B. napus and that BnACBP2 and BnACBP6 are potentially important for oil accumulation.
Persistent Identifierhttp://hdl.handle.net/10722/279904
ISSN
2017 Impact Factor: 4.059
2015 SCImago Journal Rankings: 2.301
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorLiao, P-
dc.contributor.authorWoodfield, HK-
dc.contributor.authorHarwood, JL-
dc.contributor.authorChye, ML-
dc.contributor.authorScofield, S-
dc.date.accessioned2019-12-23T08:23:29Z-
dc.date.available2019-12-23T08:23:29Z-
dc.date.issued2019-
dc.identifier.citationPlant and Cell Physiology, 2019, v. 60 n. 12, p. 2812-2825-
dc.identifier.issn0032-0781-
dc.identifier.urihttp://hdl.handle.net/10722/279904-
dc.description.abstractProduction of vegetable oils is a vital agricultural resource and oilseed rape (Brassica napus) is the third most important oil crop globally. Although the regulation of lipid biosynthesis in oilseeds is still not fully defined, the acyl-CoA-binding proteins (ACBPs) have been reported to be involved in such metabolism, including oil accumulation, in several plant species. In this study, progressive changes in gene expression in embryos and seed coats at different stages of seed development were comprehensively investigated by transcriptomic analyses in B. napus, revealing dynamic changes in the expression of genes involved in lipid biosynthesis. We show that genes encoding BnACBP proteins show distinct changes in expression at different developmental stages of seed development and show markedly different expression between embryos and seed coats. Both isoforms of the ankyrin-repeat BnACBP2 increased during the oil accumulation period of embryo development. By contrast, the expression of the three most abundant isoforms of the small molecular mass BnACBP6 in embryos showed progressive reduction, despite having the highest overall expression level. In seed coats, BnACBP3, BnACBP4 and BnACBP5 expression remained constant during development, whereas the two major isoforms of BnACBP6 increased, contrasting with the data from embryos. We conclude that genes related to fatty acid and triacylglycerol biosynthesis showing dynamic expression changes may regulate the lipid distribution in embryos and seed coats of B. napus and that BnACBP2 and BnACBP6 are potentially important for oil accumulation.-
dc.languageeng-
dc.publisherOxford University Press. The Journal's web site is located at http://pcp.oxfordjournals.org/-
dc.relation.ispartofPlant and Cell Physiology-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectBrassica napus-
dc.subjectACBP-
dc.subjectEmbryos-
dc.subjectFatty acid-
dc.subjectOilseed rape-
dc.titleComparative transcriptomics analysis of Brassica napus L. during seed maturation reveals dynamic changes in gene expression between embryos and seed coats and distinct expression profiles of acyl-CoA-binding proteins for lipid accumulation-
dc.typeArticle-
dc.identifier.emailChye, ML: mlchye@hku.hk-
dc.identifier.authorityChye, ML=rp00687-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1093/pcp/pcz169-
dc.identifier.pmid31504915-
dc.identifier.pmcidPMC6896696-
dc.identifier.scopuseid_2-s2.0-85076197729-
dc.identifier.hkuros308848-
dc.identifier.volume60-
dc.identifier.issue12-
dc.identifier.spage2812-
dc.identifier.epage2825-
dc.publisher.placeUnited Kingdom-

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