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Article: Minocycline hydrochloride loaded graphene oxide enables enhanced osteogenic activity in the presence of Gram-positive bacteria, Staphylococcus aureus

TitleMinocycline hydrochloride loaded graphene oxide enables enhanced osteogenic activity in the presence of Gram-positive bacteria, Staphylococcus aureus
Authors
KeywordsBone
Cell culture
Gene expression
Graphene
Metal implants
Issue Date2019
PublisherRoyal Society of Chemistry. The Journal's web site is located at http://pubs.rsc.org/en/journals/journalissues/tb#!recentarticles&all
Citation
Journal of Materials Chemistry B, 2019, v. 7 n. 22, p. 3590-3598 How to Cite?
AbstractImplant failures still happen because of bacterial infections and inferior osteogenic properties. Graphene oxide (GO) could induce osteogenic differentiation as well as exhibit superior antibacterial activity which may provide an answer for this issue. In this work, GO films loaded with minocycline hydrochloride (MH) were fabricated on titanium implant surfaces, and the antibacterial activity and osteogenic properties were studied in the presence of S. aureus and rat bone mesenchymal stem cells (rBMSCs) in in vitro and in vivo environments, respectively. The results indicated that the MH loaded GO films on titanium surfaces (MH&GO@Ti) exhibited enhanced osteogenic activity in vitro with improved alkaline phosphatase (ALP) activity and enhanced osteogenic related gene expressions including ALP, collagen-I, osteocalcin, and osteopontin. The in vitro results of the co-culture of rBMSCs and S. aureus indicated that the rBMSCs grew well on the MH&GO@Ti sample with a higher coverage of cells and the bacteria were almost unobservable on the surface. In the in vivo experiments, the MH&GO@Ti exhibited excellent antibacterial and osteogenic activities in the presence of bacteria. No inflammatory cells for example neutrophils were found and superior bone–implant integration was obtained. This study offers a new proposal for the clinical application of drug-loaded graphene coating.
Persistent Identifierhttp://hdl.handle.net/10722/278241
ISSN
2017 Impact Factor: 4.776

 

DC FieldValueLanguage
dc.contributor.authorQiu, J-
dc.contributor.authorQIAN, W-
dc.contributor.authorZHANG, J-
dc.contributor.authorCHEN, D-
dc.contributor.authorYeung, KWK-
dc.contributor.authorLIU, X-
dc.date.accessioned2019-10-04T08:10:12Z-
dc.date.available2019-10-04T08:10:12Z-
dc.date.issued2019-
dc.identifier.citationJournal of Materials Chemistry B, 2019, v. 7 n. 22, p. 3590-3598-
dc.identifier.issn2050-750X-
dc.identifier.urihttp://hdl.handle.net/10722/278241-
dc.description.abstractImplant failures still happen because of bacterial infections and inferior osteogenic properties. Graphene oxide (GO) could induce osteogenic differentiation as well as exhibit superior antibacterial activity which may provide an answer for this issue. In this work, GO films loaded with minocycline hydrochloride (MH) were fabricated on titanium implant surfaces, and the antibacterial activity and osteogenic properties were studied in the presence of S. aureus and rat bone mesenchymal stem cells (rBMSCs) in in vitro and in vivo environments, respectively. The results indicated that the MH loaded GO films on titanium surfaces (MH&GO@Ti) exhibited enhanced osteogenic activity in vitro with improved alkaline phosphatase (ALP) activity and enhanced osteogenic related gene expressions including ALP, collagen-I, osteocalcin, and osteopontin. The in vitro results of the co-culture of rBMSCs and S. aureus indicated that the rBMSCs grew well on the MH&GO@Ti sample with a higher coverage of cells and the bacteria were almost unobservable on the surface. In the in vivo experiments, the MH&GO@Ti exhibited excellent antibacterial and osteogenic activities in the presence of bacteria. No inflammatory cells for example neutrophils were found and superior bone–implant integration was obtained. This study offers a new proposal for the clinical application of drug-loaded graphene coating.-
dc.languageeng-
dc.publisherRoyal Society of Chemistry. The Journal's web site is located at http://pubs.rsc.org/en/journals/journalissues/tb#!recentarticles&all-
dc.relation.ispartofJournal of Materials Chemistry B-
dc.subjectBone-
dc.subjectCell culture-
dc.subjectGene expression-
dc.subjectGraphene-
dc.subjectMetal implants-
dc.titleMinocycline hydrochloride loaded graphene oxide enables enhanced osteogenic activity in the presence of Gram-positive bacteria, Staphylococcus aureus-
dc.typeArticle-
dc.identifier.emailYeung, KWK: wkkyeung@hku.hk-
dc.identifier.authorityYeung, KWK=rp00309-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1039/C9TB00405J-
dc.identifier.scopuseid_2-s2.0-85066854915-
dc.identifier.hkuros307083-
dc.identifier.volume7-
dc.identifier.issue22-
dc.identifier.spage3590-
dc.identifier.epage3598-
dc.publisher.placeUnited Kingdom-

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