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Article: Enhancing the antibacterial efficacy of low-dose gentamicin with 5 minute assistance of photothermy at 50 °C

TitleEnhancing the antibacterial efficacy of low-dose gentamicin with 5 minute assistance of photothermy at 50 °C
Authors
KeywordsAntibiotics
Biosynthesis
Cell adhesion
Cell culture
Coatings
Issue Date2019
PublisherRoyal Society of Chemistry. The Journal's web site is located at http://pubs.rsc.org/en/Journals/JournalIssues/bm#!recentarticles&all
Citation
Biomaterials Science, 2019, v. 7 n. 4, p. 1437-1447 How to Cite?
AbstractImplant materials are prone to bacterial infections and cause serious consequences, while traditional antibiotic therapy has a long treatment cycle and even causes bacterial resistance. In this work, a photothermal therapy (PTT) assisted drug release system has been developed on the implant surface for in situ rapid disinfection under 808 nm light irradiation within a short time, in which gentamicin (Gent) is loaded by polyethylene glycol (PEG) modified molybdenum disulfide (MoS2) on Ti surface, and then encapsulated with chitosan (CS) (CS/Gent/PEG/MoS2-Ti). The hyperthermia produced by the coatings irradiated by 808 nm near-infrared (NIR) light can not only accelerate the local release of Gent, but also reduce the activity of bacteria, which makes it easy for these locally released drugs to enter the interior of the bacteria to inhibit the protein synthesis and destroy the cell membrane. When maintained at 50 °C for 5 min under NIR irradiation, this system can achieve an antibacterial efficacy of 99.93% and 99.19% against Escherichia coli and Staphylococcus aureus, respectively. By contrast, even after treatment for 120 min, only a 93.79% antibacterial ratio can be obtained for Gent alone. This is because hyperthermia produced from the coatings during irradiation can assist antibiotics in killing bacteria in a short time. Even under a low dose of 2 μg mL−1, the photothermal effect assisted gentamicin can achieve an antibacterial efficacy of 96.86% within 5 min. In vitro cell culture shows that the modified surface can facilitate cell adhesion, spreading and proliferation. The 7 day subcutaneous infection model confirms that the prepared surface system can exhibit a much faster sterilization and tissue reconstruction than the control group with light assistance. Compared with the traditional drug release system, this photothermy controlled drug-loaded implant surface system can not only provide rapid and high-efficiency in situ sterilization, but also offer long-term prevention of local bacterial infection.
Persistent Identifierhttp://hdl.handle.net/10722/277992
ISSN
2019 Impact Factor: 6.183
2015 SCImago Journal Rankings: 1.347

 

DC FieldValueLanguage
dc.contributor.authorMA, M-
dc.contributor.authorLIU, X-
dc.contributor.authorTAN, L-
dc.contributor.authorCUI, Z-
dc.contributor.authorYANG, X-
dc.contributor.authorLIANG, Y-
dc.contributor.authorLI, Z-
dc.contributor.authorZHENG, Y-
dc.contributor.authorYeung, KWK-
dc.contributor.authorWU, S-
dc.date.accessioned2019-10-04T08:05:20Z-
dc.date.available2019-10-04T08:05:20Z-
dc.date.issued2019-
dc.identifier.citationBiomaterials Science, 2019, v. 7 n. 4, p. 1437-1447-
dc.identifier.issn2047-4830-
dc.identifier.urihttp://hdl.handle.net/10722/277992-
dc.description.abstractImplant materials are prone to bacterial infections and cause serious consequences, while traditional antibiotic therapy has a long treatment cycle and even causes bacterial resistance. In this work, a photothermal therapy (PTT) assisted drug release system has been developed on the implant surface for in situ rapid disinfection under 808 nm light irradiation within a short time, in which gentamicin (Gent) is loaded by polyethylene glycol (PEG) modified molybdenum disulfide (MoS2) on Ti surface, and then encapsulated with chitosan (CS) (CS/Gent/PEG/MoS2-Ti). The hyperthermia produced by the coatings irradiated by 808 nm near-infrared (NIR) light can not only accelerate the local release of Gent, but also reduce the activity of bacteria, which makes it easy for these locally released drugs to enter the interior of the bacteria to inhibit the protein synthesis and destroy the cell membrane. When maintained at 50 °C for 5 min under NIR irradiation, this system can achieve an antibacterial efficacy of 99.93% and 99.19% against Escherichia coli and Staphylococcus aureus, respectively. By contrast, even after treatment for 120 min, only a 93.79% antibacterial ratio can be obtained for Gent alone. This is because hyperthermia produced from the coatings during irradiation can assist antibiotics in killing bacteria in a short time. Even under a low dose of 2 μg mL−1, the photothermal effect assisted gentamicin can achieve an antibacterial efficacy of 96.86% within 5 min. In vitro cell culture shows that the modified surface can facilitate cell adhesion, spreading and proliferation. The 7 day subcutaneous infection model confirms that the prepared surface system can exhibit a much faster sterilization and tissue reconstruction than the control group with light assistance. Compared with the traditional drug release system, this photothermy controlled drug-loaded implant surface system can not only provide rapid and high-efficiency in situ sterilization, but also offer long-term prevention of local bacterial infection.-
dc.languageeng-
dc.publisherRoyal Society of Chemistry. The Journal's web site is located at http://pubs.rsc.org/en/Journals/JournalIssues/bm#!recentarticles&all-
dc.relation.ispartofBiomaterials Science-
dc.subjectAntibiotics-
dc.subjectBiosynthesis-
dc.subjectCell adhesion-
dc.subjectCell culture-
dc.subjectCoatings-
dc.titleEnhancing the antibacterial efficacy of low-dose gentamicin with 5 minute assistance of photothermy at 50 °C-
dc.typeArticle-
dc.identifier.emailYeung, KWK: wkkyeung@hku.hk-
dc.identifier.authorityYeung, KWK=rp00309-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1039/C8BM01539B-
dc.identifier.pmid30666993-
dc.identifier.scopuseid_2-s2.0-85063443375-
dc.identifier.hkuros306967-
dc.identifier.volume7-
dc.identifier.issue4-
dc.identifier.spage1437-
dc.identifier.epage1447-
dc.publisher.placeUnited Kingdom-

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