File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Grass carp prolactin gene: Structural characterization and signal transduction for PACAP-induced prolactin promoter activity

TitleGrass carp prolactin gene: Structural characterization and signal transduction for PACAP-induced prolactin promoter activity
Authors
Keywordsanimal
carp
drug effect
gene expression regulation
genetic transcription
Issue Date2018
PublisherNature Research (part of Springer Nature): Fully open access journals. The Journal's web site is located at http://www.nature.com/srep/index.html
Citation
Scientific Reports, 2018, v. 8 n. 1, p. article no. 4655 How to Cite?
AbstractIn this study, structural analysis of grass carp prolactin (PRL) gene was performed and the signaling mechanisms for pituitary adenylate cyclase-activating peptide (PACAP) regulation of PRL promoter activity were investigated. In αT3-1 cells, PRL promoter activity could be induced by oPACAP38 which was blocked by PACAP antagonist but not the VIP antagonist. The stimulatory effect of oPACAP38 was mimicked by activation of AC/cAMP and voltage-sensitive Ca2+ channel (VSCC) signaling, or induction of Ca2+ entry. In parallel, PACAP-induced PRL promoter activity was negated or inhibited by suppressing cAMP production, inhibiting PKA activity, removal of extracellular Ca2+, VSCC blockade, calmodulin (CaM) antagonism, and inactivation of CaM kinase II. Similar sensitivity to L-type VSCC, CaM and CaM kinase II inhibition were also observed by substituting cAMP analog for oPACAP38 as the stimulant for PRL promoter activity. Moreover, PACAP-induced PRL promoter activity was also blocked by inhibition of PLC signaling, attenuation of [Ca2+]i immobilization via IP3 receptors, and blockade of PI3K/P70S6K pathway. The PACAP-induced PRL promoter activation may involve transactivation of the transcription factor CREB. These results suggest that PACAP can stimulate PRL promoter activation by PAC1 mediated functional coupling of the Ca2+/CaM/CaM kinase II cascades with the AC/cAMP/PKA pathway. Apparently, other signaling pathways, including PLC/IP3 and PI3K/P70S6K cascades, may also be involved in PACAP induction of PRL gene transcription.
Persistent Identifierhttp://hdl.handle.net/10722/277899
ISSN
2017 Impact Factor: 4.122
2015 SCImago Journal Rankings: 2.073
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorLin, C-
dc.contributor.authorBai, J-
dc.contributor.authorHe, M-
dc.contributor.authorWong, AOL-
dc.date.accessioned2019-10-04T08:03:33Z-
dc.date.available2019-10-04T08:03:33Z-
dc.date.issued2018-
dc.identifier.citationScientific Reports, 2018, v. 8 n. 1, p. article no. 4655-
dc.identifier.issn2045-2322-
dc.identifier.urihttp://hdl.handle.net/10722/277899-
dc.description.abstractIn this study, structural analysis of grass carp prolactin (PRL) gene was performed and the signaling mechanisms for pituitary adenylate cyclase-activating peptide (PACAP) regulation of PRL promoter activity were investigated. In αT3-1 cells, PRL promoter activity could be induced by oPACAP38 which was blocked by PACAP antagonist but not the VIP antagonist. The stimulatory effect of oPACAP38 was mimicked by activation of AC/cAMP and voltage-sensitive Ca2+ channel (VSCC) signaling, or induction of Ca2+ entry. In parallel, PACAP-induced PRL promoter activity was negated or inhibited by suppressing cAMP production, inhibiting PKA activity, removal of extracellular Ca2+, VSCC blockade, calmodulin (CaM) antagonism, and inactivation of CaM kinase II. Similar sensitivity to L-type VSCC, CaM and CaM kinase II inhibition were also observed by substituting cAMP analog for oPACAP38 as the stimulant for PRL promoter activity. Moreover, PACAP-induced PRL promoter activity was also blocked by inhibition of PLC signaling, attenuation of [Ca2+]i immobilization via IP3 receptors, and blockade of PI3K/P70S6K pathway. The PACAP-induced PRL promoter activation may involve transactivation of the transcription factor CREB. These results suggest that PACAP can stimulate PRL promoter activation by PAC1 mediated functional coupling of the Ca2+/CaM/CaM kinase II cascades with the AC/cAMP/PKA pathway. Apparently, other signaling pathways, including PLC/IP3 and PI3K/P70S6K cascades, may also be involved in PACAP induction of PRL gene transcription.-
dc.languageeng-
dc.publisherNature Research (part of Springer Nature): Fully open access journals. The Journal's web site is located at http://www.nature.com/srep/index.html-
dc.relation.ispartofScientific Reports-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectanimal-
dc.subjectcarp-
dc.subjectdrug effect-
dc.subjectgene expression regulation-
dc.subjectgenetic transcription-
dc.titleGrass carp prolactin gene: Structural characterization and signal transduction for PACAP-induced prolactin promoter activity-
dc.typeArticle-
dc.identifier.emailHe, M: hemu@hkucc.hku.hk-
dc.identifier.emailWong, AOL: olwong@hku.hk-
dc.identifier.authorityWong, AOL=rp00806-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/s41598-018-23092-0-
dc.identifier.pmid29545542-
dc.identifier.pmcidPMC5854708-
dc.identifier.scopuseid_2-s2.0-85044176827-
dc.identifier.hkuros306668-
dc.identifier.volume8-
dc.identifier.issue1-
dc.identifier.spagearticle no. 4655-
dc.identifier.epagearticle no. 4655-
dc.publisher.placeUnited Kingdom-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats