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Article: Functional genomic analyses of exopolysaccharide-producing streptococcus thermophilus ASCC 1275 in response to milk fermentation conditions

TitleFunctional genomic analyses of exopolysaccharide-producing streptococcus thermophilus ASCC 1275 in response to milk fermentation conditions
Authors
KeywordsExopolysaccharide
PH
Proteome
Streptococcus thermophilus
Transcriptome
Issue Date2019
PublisherFrontiers Research Foundation.
Citation
Frontiers in Microbiology - Food Microbiology, 2019, v. 10 n. Aug, article no. 1975 How to Cite?
AbstractExopolysaccharide (EPS) produced from dairy bacteria improves texture and functionalities of fermented dairy foods. Our previous study showed improved EPS production from Streptococcus thermophilus ASCC1275 (ST1275) by simple alteration of fermentation conditions such as pH decrease (pH 6.5 → pH 5.5), temperature increase (37◦ C → 40◦ C) and/or whey protein isolate (WPI) supplementation. The iTRAQ-based proteomics in combination with transcriptomics were applied to understand cellular protein expression in ST1275 in response to above shifts during milk fermentation. The pH decrease induced the most differentially expressed proteins (DEPs) that are involved in cellular metabolic responses including glutamate catabolism, arginine biosynthesis, cysteine catabolism, purine metabolism, lactose uptake, and fatty acid biosynthesis. Temperature increase and WPI supplementation did not induce much changes in global protein express profiles of ST1275 between comparisons of pH 5.5 conditions. Comparative proteomic analyses from pairwise comparisons demonstrated enhanced glutamate catabolism and purine metabolism under pH 5.5 conditions (Cd2, Cd3, and Cd4) compared to that of pH 6.5 condition (Cd1). Concordance analysis for differential expressed genes (DEGs) and DEPs highlighted down-regulated glutamate catabolism and up-regulated arginine biosynthesis in pH 5.5 conditions. Down regulation of glutamate catabolism was also confirmed by pathway enrichment analysis. Down-regulation of EpsB involved in EPS assembly was observed at both mRNA and protein level in pH 5.5 conditions compared to that in pH 6.5 condition. Medium pH decreased to mild acidic level induced cellular changes associated with glutamate catabolism, arginine biosynthesis and regulation of EPS assembly in ST1275. © 2019 Wu, Chu, Padmanabhan and Shah.
Persistent Identifierhttp://hdl.handle.net/10722/277593
ISSN
2017 Impact Factor: 4.019
2015 SCImago Journal Rankings: 1.970
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWu, Q-
dc.contributor.authorPraveul, AP-
dc.contributor.authorShah, N-
dc.date.accessioned2019-09-20T08:53:59Z-
dc.date.available2019-09-20T08:53:59Z-
dc.date.issued2019-
dc.identifier.citationFrontiers in Microbiology - Food Microbiology, 2019, v. 10 n. Aug, article no. 1975-
dc.identifier.issn1664-302X-
dc.identifier.urihttp://hdl.handle.net/10722/277593-
dc.description.abstractExopolysaccharide (EPS) produced from dairy bacteria improves texture and functionalities of fermented dairy foods. Our previous study showed improved EPS production from Streptococcus thermophilus ASCC1275 (ST1275) by simple alteration of fermentation conditions such as pH decrease (pH 6.5 → pH 5.5), temperature increase (37◦ C → 40◦ C) and/or whey protein isolate (WPI) supplementation. The iTRAQ-based proteomics in combination with transcriptomics were applied to understand cellular protein expression in ST1275 in response to above shifts during milk fermentation. The pH decrease induced the most differentially expressed proteins (DEPs) that are involved in cellular metabolic responses including glutamate catabolism, arginine biosynthesis, cysteine catabolism, purine metabolism, lactose uptake, and fatty acid biosynthesis. Temperature increase and WPI supplementation did not induce much changes in global protein express profiles of ST1275 between comparisons of pH 5.5 conditions. Comparative proteomic analyses from pairwise comparisons demonstrated enhanced glutamate catabolism and purine metabolism under pH 5.5 conditions (Cd2, Cd3, and Cd4) compared to that of pH 6.5 condition (Cd1). Concordance analysis for differential expressed genes (DEGs) and DEPs highlighted down-regulated glutamate catabolism and up-regulated arginine biosynthesis in pH 5.5 conditions. Down regulation of glutamate catabolism was also confirmed by pathway enrichment analysis. Down-regulation of EpsB involved in EPS assembly was observed at both mRNA and protein level in pH 5.5 conditions compared to that in pH 6.5 condition. Medium pH decreased to mild acidic level induced cellular changes associated with glutamate catabolism, arginine biosynthesis and regulation of EPS assembly in ST1275. © 2019 Wu, Chu, Padmanabhan and Shah.-
dc.languageeng-
dc.publisherFrontiers Research Foundation.-
dc.relation.ispartofFrontiers in Microbiology - Food Microbiology-
dc.rightsThis Document is Protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectExopolysaccharide-
dc.subjectPH-
dc.subjectProteome-
dc.subjectStreptococcus thermophilus-
dc.subjectTranscriptome-
dc.titleFunctional genomic analyses of exopolysaccharide-producing streptococcus thermophilus ASCC 1275 in response to milk fermentation conditions-
dc.typeArticle-
dc.identifier.emailShah, N: npshah@hku.hk-
dc.identifier.authorityShah, N=rp01571-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3389/fmicb.2019.01975-
dc.identifier.pmid31507577-
dc.identifier.scopuseid_2-s2.0-85071920263-
dc.identifier.hkuros305582-
dc.identifier.volume10-
dc.identifier.issueAug-
dc.identifier.spagearticle no. 1975-
dc.identifier.epagearticle no. 1975-
dc.identifier.isiWOS:000482414100001-
dc.publisher.placeSwitzerland-

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