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Article: On‐Demand Droplet Collection for Capturing Single Cells

TitleOn‐Demand Droplet Collection for Capturing Single Cells
Authors
KeywordsDroplet microfluidics
Quantitative collection
Single cell
Issue Date2019
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jabout/107640323/2421_info.html
Citation
Small, 2019 How to Cite?
AbstractDroplet‐based microfluidic techniques are extensively used in efficient manipulation and genome‐wide analysis of individual cells, probing the heterogeneity among populations of individuals. However, the extraction and isolation of single cells from individual droplets remains difficult due to the inevitable sample loss during processing. Herein, an automated system for accurate collection of defined numbers of droplets containing single cells is presented. Based on alternate sorting and dispensing in three branch channels, the droplet number can be precisely controlled down to single‐droplet resolution. While encapsulating single cells and reserving one branch as a waste channel, sorting can be seamlessly integrated to enable on‐demand collection of single cells. Combined with a lossless recovery strategy, this technique achieves capture and culture of individual cells with a harvest rate of over 95%. The on‐demand droplet collection technique has great potential to realize quantitative processing and analysis of single cells for elucidating the role of cell‐to‐cell variations.
Persistent Identifierhttp://hdl.handle.net/10722/277488
ISSN
2017 Impact Factor: 9.598
2015 SCImago Journal Rankings: 3.401
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorNan, L-
dc.contributor.authorLai, MYA-
dc.contributor.authorTang, MYH-
dc.contributor.authorChan, YK-
dc.contributor.authorPoon, LLM-
dc.contributor.authorShum, HC-
dc.date.accessioned2019-09-20T08:52:01Z-
dc.date.available2019-09-20T08:52:01Z-
dc.date.issued2019-
dc.identifier.citationSmall, 2019-
dc.identifier.issn1613-6810-
dc.identifier.urihttp://hdl.handle.net/10722/277488-
dc.description.abstractDroplet‐based microfluidic techniques are extensively used in efficient manipulation and genome‐wide analysis of individual cells, probing the heterogeneity among populations of individuals. However, the extraction and isolation of single cells from individual droplets remains difficult due to the inevitable sample loss during processing. Herein, an automated system for accurate collection of defined numbers of droplets containing single cells is presented. Based on alternate sorting and dispensing in three branch channels, the droplet number can be precisely controlled down to single‐droplet resolution. While encapsulating single cells and reserving one branch as a waste channel, sorting can be seamlessly integrated to enable on‐demand collection of single cells. Combined with a lossless recovery strategy, this technique achieves capture and culture of individual cells with a harvest rate of over 95%. The on‐demand droplet collection technique has great potential to realize quantitative processing and analysis of single cells for elucidating the role of cell‐to‐cell variations.-
dc.languageeng-
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jabout/107640323/2421_info.html-
dc.relation.ispartofSmall-
dc.subjectDroplet microfluidics-
dc.subjectQuantitative collection-
dc.subjectSingle cell-
dc.titleOn‐Demand Droplet Collection for Capturing Single Cells-
dc.typeArticle-
dc.identifier.emailTang, MYH: sdmt99@connect.hku.hk-
dc.identifier.emailChan, YK: josephyk@connect.hku.hk-
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hk-
dc.identifier.emailShum, HC: ashum@hku.hk-
dc.identifier.authorityChan, YK=rp02536-
dc.identifier.authorityPoon, LLM=rp00484-
dc.identifier.authorityShum, HC=rp01439-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/smll.201902889-
dc.identifier.pmid31448532-
dc.identifier.scopuseid_2-s2.0-85071110480-
dc.identifier.hkuros305917-
dc.identifier.isiWOS:000482639800001-
dc.publisher.placeGermany-

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