Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1186/s12985-018-1059-7
- Scopus: eid_2-s2.0-85054104020
- PMID: 30261891
- WOS: WOS:000445762400001
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus
Title | Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus |
---|---|
Authors | |
Keywords | Human adenovirus Real-time PCR RealStar® Adenovirus PCR Kit |
Issue Date | 2018 |
Publisher | BioMed Central Ltd. The Journal's web site is located at http://www.virologyj.com/home/ |
Citation | Virology Journal, 2018, v. 15 n. 1, p. article no. 149 How to Cite? |
Abstract | Background: Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods: The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results: Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 log 10 (plasma) and 2.94 to 9 log 10 (viral transport medium) copies/mL, with the coefficient of determination (R 2 ) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 log 10 and 2.60 log 10 copies/mL and those for viral transport medium were 2.31 log 10 and 2.94 log 10 copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit (R 2 = 0.984), with an average bias of - 0.16 log 10 copies/mL. Conclusions: The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit. © 2018 The Author(s). |
Persistent Identifier | http://hdl.handle.net/10722/276335 |
ISSN | 2023 Impact Factor: 4.0 2023 SCImago Journal Rankings: 1.016 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wong, SSY | - |
dc.contributor.author | Yip, CY | - |
dc.contributor.author | Sridhar, S | - |
dc.contributor.author | Leung, KH | - |
dc.contributor.author | Cheng, AKW | - |
dc.contributor.author | Fung, AMY | - |
dc.contributor.author | Lam, HY | - |
dc.contributor.author | Chan, KH | - |
dc.contributor.author | Chan, JFW | - |
dc.contributor.author | Cheng, CCV | - |
dc.contributor.author | Tang, BSF | - |
dc.contributor.author | Yuen, KY | - |
dc.date.accessioned | 2019-09-10T03:01:01Z | - |
dc.date.available | 2019-09-10T03:01:01Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Virology Journal, 2018, v. 15 n. 1, p. article no. 149 | - |
dc.identifier.issn | 1743-422X | - |
dc.identifier.uri | http://hdl.handle.net/10722/276335 | - |
dc.description.abstract | Background: Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods: The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results: Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 log 10 (plasma) and 2.94 to 9 log 10 (viral transport medium) copies/mL, with the coefficient of determination (R 2 ) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 log 10 and 2.60 log 10 copies/mL and those for viral transport medium were 2.31 log 10 and 2.94 log 10 copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit (R 2 = 0.984), with an average bias of - 0.16 log 10 copies/mL. Conclusions: The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit. © 2018 The Author(s). | - |
dc.language | eng | - |
dc.publisher | BioMed Central Ltd. The Journal's web site is located at http://www.virologyj.com/home/ | - |
dc.relation.ispartof | Virology Journal | - |
dc.rights | Virology Journal. Copyright © BioMed Central Ltd. | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Human adenovirus | - |
dc.subject | Real-time PCR | - |
dc.subject | RealStar® Adenovirus PCR Kit | - |
dc.title | Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus | - |
dc.type | Article | - |
dc.identifier.email | Wong, SSY: samsonsy@hku.hk | - |
dc.identifier.email | Yip, CY: yipcyril@hku.hk | - |
dc.identifier.email | Sridhar, S: sid8998@hku.hk | - |
dc.identifier.email | Leung, KH: khl17@hku.hk | - |
dc.identifier.email | Chan, KH: chankh2@hkucc.hku.hk | - |
dc.identifier.email | Chan, JFW: jfwchan@hku.hk | - |
dc.identifier.email | Cheng, CCV: vcccheng@hkucc.hku.hk | - |
dc.identifier.email | Yuen, KY: kyyuen@hkucc.hku.hk | - |
dc.identifier.authority | Wong, SSY=rp00395 | - |
dc.identifier.authority | Yip, CY=rp01721 | - |
dc.identifier.authority | Sridhar, S=rp02249 | - |
dc.identifier.authority | Chan, KH=rp01921 | - |
dc.identifier.authority | Chan, JFW=rp01736 | - |
dc.identifier.authority | Yuen, KY=rp00366 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1186/s12985-018-1059-7 | - |
dc.identifier.pmid | 30261891 | - |
dc.identifier.scopus | eid_2-s2.0-85054104020 | - |
dc.identifier.hkuros | 303322 | - |
dc.identifier.volume | 15 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | article no. 149 | - |
dc.identifier.epage | article no. 149 | - |
dc.identifier.isi | WOS:000445762400001 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 1743-422X | - |