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Article: Palmitate and stearate are increased in the plasma in a 6-OHDA model of Parkinson’s disease

TitlePalmitate and stearate are increased in the plasma in a 6-OHDA model of Parkinson’s disease
Authors
KeywordsParkinson’s disease
6-OHDA
GC-MS
plasma
midbrain
Issue Date2019
PublisherMDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/metabolites
Citation
Metabolites, 2019, v. 9 n. 2, p. article no. 31 How to Cite?
AbstractIntroduction: Parkinson’s disease (PD) is the second most common neurodegenerative disorder, without any widely available curative therapy. Metabolomics is a powerful tool which can be used to identify unexpected pathway-related disease progression and pathophysiological mechanisms. In this study, metabolomics in brain, plasma and liver was investigated in an experimental PD model, to discover small molecules that are associated with dopaminergic cell loss. Methods: Sprague Dawley (SD) rats were injected unilaterally with 6-hydroxydopamine (6-OHDA) or saline for the vehicle control group into the medial forebrain bundle (MFB) to induce loss of dopaminergic neurons in the substantia nigra pars compacta. Plasma, midbrain and liver samples were collected for metabolic profiling. Multivariate and univariate analyses revealed metabolites that were altered in the PD group. Results: In plasma, palmitic acid (q = 3.72 × 10−2, FC = 1.81) and stearic acid (q = 3.84 × 10−2, FC = 2.15), were found to be increased in the PD group. Palmitic acid (q = 3.5 × 10−2) and stearic acid (q = 2.7 × 10−2) correlated with test scores indicative of motor dysfunction. Monopalmitin (q = 4.8 × 10−2, FC = −11.7), monostearin (q = 3.72 × 10−2, FC = −15.1) and myo-inositol (q = 3.81 × 10−2, FC = −3.32), were reduced in the midbrain. The liver did not have altered levels of these molecules. Conclusion: Our results show that saturated free fatty acids, their monoglycerides and myo-inositol metabolism in the midbrain and enteric circulation are associated with 6-OHDA-induced PD pathology.
Persistent Identifierhttp://hdl.handle.net/10722/276233
ISSN
2015 SCImago Journal Rankings: 0.124
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorSHAH, A-
dc.contributor.authorHan, P-
dc.contributor.authorWong, MY-
dc.contributor.authorChang, RCC-
dc.contributor.authorLegido-Quigley, C-
dc.date.accessioned2019-09-10T02:58:42Z-
dc.date.available2019-09-10T02:58:42Z-
dc.date.issued2019-
dc.identifier.citationMetabolites, 2019, v. 9 n. 2, p. article no. 31-
dc.identifier.issn2218-1989-
dc.identifier.urihttp://hdl.handle.net/10722/276233-
dc.description.abstractIntroduction: Parkinson’s disease (PD) is the second most common neurodegenerative disorder, without any widely available curative therapy. Metabolomics is a powerful tool which can be used to identify unexpected pathway-related disease progression and pathophysiological mechanisms. In this study, metabolomics in brain, plasma and liver was investigated in an experimental PD model, to discover small molecules that are associated with dopaminergic cell loss. Methods: Sprague Dawley (SD) rats were injected unilaterally with 6-hydroxydopamine (6-OHDA) or saline for the vehicle control group into the medial forebrain bundle (MFB) to induce loss of dopaminergic neurons in the substantia nigra pars compacta. Plasma, midbrain and liver samples were collected for metabolic profiling. Multivariate and univariate analyses revealed metabolites that were altered in the PD group. Results: In plasma, palmitic acid (q = 3.72 × 10−2, FC = 1.81) and stearic acid (q = 3.84 × 10−2, FC = 2.15), were found to be increased in the PD group. Palmitic acid (q = 3.5 × 10−2) and stearic acid (q = 2.7 × 10−2) correlated with test scores indicative of motor dysfunction. Monopalmitin (q = 4.8 × 10−2, FC = −11.7), monostearin (q = 3.72 × 10−2, FC = −15.1) and myo-inositol (q = 3.81 × 10−2, FC = −3.32), were reduced in the midbrain. The liver did not have altered levels of these molecules. Conclusion: Our results show that saturated free fatty acids, their monoglycerides and myo-inositol metabolism in the midbrain and enteric circulation are associated with 6-OHDA-induced PD pathology.-
dc.languageeng-
dc.publisherMDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/metabolites-
dc.relation.ispartofMetabolites-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectParkinson’s disease-
dc.subject6-OHDA-
dc.subjectGC-MS-
dc.subjectplasma-
dc.subjectmidbrain-
dc.titlePalmitate and stearate are increased in the plasma in a 6-OHDA model of Parkinson’s disease-
dc.typeArticle-
dc.identifier.emailChang, RCC: rccchang@hku.hk-
dc.identifier.authorityChang, RCC=rp00470-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3390/metabo9020031-
dc.identifier.pmid30781729-
dc.identifier.pmcidPMC6409985-
dc.identifier.scopuseid_2-s2.0-85071053925-
dc.identifier.hkuros303972-
dc.identifier.volume9-
dc.identifier.issue2-
dc.identifier.spagearticle no. 31-
dc.identifier.epagearticle no. 31-
dc.publisher.placeSwitzerland-

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