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Article: RNase H Meets Molecular Beacons: An Ultrasensitive Fluorometric Assay for Nucleic Acids

TitleRNase H Meets Molecular Beacons: An Ultrasensitive Fluorometric Assay for Nucleic Acids
Authors
KeywordsDNA
Detection limit
Exonuclease III
Sensitivity
Selectivity
T7 exonuclease
Signal amplification
Issue Date2018
PublisherSpringer-Verlag Wien. The Journal's web site is located at http://www.springer.at/mikro
Citation
Microchimica Acta, 2018, v. 185 n. 8, article no. 375 How to Cite?
AbstractAn innovative signal amplification strategy assisted by RNase H is described here for the detection of DNA targets in a universal fashion. A tailor-made RNA molecular beacon (RMB) conjugated with a fluorescence resonance energy transfer (FRET) pair (fluorophore and quencher) was designed, characterized, and combined with the employment of RNase H. Its performance is compared to that of other nucleases including Exonuclease III and T7 exonuclease. Fluorometry, performed best at excitation/emission wavelengths of 490/520 nm, gives an amazingly low detection limit of 23 fM for target DNA. The method was verified by the determination of human hemochromatosis (HFE) gene. It is perceived that the method represents a versatile tool for the detection of a wide range of targets.
Persistent Identifierhttp://hdl.handle.net/10722/275452
ISSN
2017 Impact Factor: 5.705
2015 SCImago Journal Rankings: 1.116
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWang, S-
dc.contributor.authorLiu, K-
dc.contributor.authorLou, Y-
dc.contributor.authorWang, S-
dc.contributor.authorPeng, Y-
dc.contributor.authorChen, J-
dc.contributor.authorHuang, J-
dc.contributor.authorXie, S-
dc.contributor.authorCui, L-
dc.contributor.authorWang, X-
dc.date.accessioned2019-09-10T02:42:50Z-
dc.date.available2019-09-10T02:42:50Z-
dc.date.issued2018-
dc.identifier.citationMicrochimica Acta, 2018, v. 185 n. 8, article no. 375-
dc.identifier.issn0026-3672-
dc.identifier.urihttp://hdl.handle.net/10722/275452-
dc.description.abstractAn innovative signal amplification strategy assisted by RNase H is described here for the detection of DNA targets in a universal fashion. A tailor-made RNA molecular beacon (RMB) conjugated with a fluorescence resonance energy transfer (FRET) pair (fluorophore and quencher) was designed, characterized, and combined with the employment of RNase H. Its performance is compared to that of other nucleases including Exonuclease III and T7 exonuclease. Fluorometry, performed best at excitation/emission wavelengths of 490/520 nm, gives an amazingly low detection limit of 23 fM for target DNA. The method was verified by the determination of human hemochromatosis (HFE) gene. It is perceived that the method represents a versatile tool for the detection of a wide range of targets.-
dc.languageeng-
dc.publisherSpringer-Verlag Wien. The Journal's web site is located at http://www.springer.at/mikro-
dc.relation.ispartofMicrochimica Acta-
dc.subjectDNA-
dc.subjectDetection limit-
dc.subjectExonuclease III-
dc.subjectSensitivity-
dc.subjectSelectivity-
dc.subjectT7 exonuclease-
dc.subjectSignal amplification-
dc.titleRNase H Meets Molecular Beacons: An Ultrasensitive Fluorometric Assay for Nucleic Acids-
dc.typeArticle-
dc.identifier.emailChen, J: abchen@hkucc.hku.hk-
dc.identifier.authorityChen, J=rp01316-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00604-018-2909-0-
dc.identifier.pmid30008087-
dc.identifier.scopuseid_2-s2.0-85050072058-
dc.identifier.hkuros304896-
dc.identifier.hkuros305029-
dc.identifier.volume185-
dc.identifier.issue8-
dc.identifier.spagearticle no. 375-
dc.identifier.epagearticle no. 375-
dc.identifier.isiWOS:000438876300001-
dc.publisher.placeAustria-

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