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- Publisher Website: 10.1007/s00604-018-2909-0
- Scopus: eid_2-s2.0-85050072058
- PMID: 30008087
- WOS: WOS:000438876300001
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Article: RNase H Meets Molecular Beacons: An Ultrasensitive Fluorometric Assay for Nucleic Acids
Title | RNase H Meets Molecular Beacons: An Ultrasensitive Fluorometric Assay for Nucleic Acids |
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Authors | |
Keywords | DNA Detection limit Exonuclease III Sensitivity Selectivity T7 exonuclease Signal amplification |
Issue Date | 2018 |
Publisher | Springer-Verlag Wien. The Journal's web site is located at http://www.springer.at/mikro |
Citation | Microchimica Acta, 2018, v. 185 n. 8, article no. 375 How to Cite? |
Abstract | An innovative signal amplification strategy assisted by RNase H is described here for the detection of DNA targets in a universal fashion. A tailor-made RNA molecular beacon (RMB) conjugated with a fluorescence resonance energy transfer (FRET) pair (fluorophore and quencher) was designed, characterized, and combined with the employment of RNase H. Its performance is compared to that of other nucleases including Exonuclease III and T7 exonuclease. Fluorometry, performed best at excitation/emission wavelengths of 490/520 nm, gives an amazingly low detection limit of 23 fM for target DNA. The method was verified by the determination of human hemochromatosis (HFE) gene. It is perceived that the method represents a versatile tool for the detection of a wide range of targets. |
Persistent Identifier | http://hdl.handle.net/10722/275452 |
ISSN | 2023 Impact Factor: 5.3 2023 SCImago Journal Rankings: 0.848 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Wang, S | - |
dc.contributor.author | Liu, K | - |
dc.contributor.author | Lou, Y | - |
dc.contributor.author | Wang, S | - |
dc.contributor.author | Peng, Y | - |
dc.contributor.author | Chen, J | - |
dc.contributor.author | Huang, J | - |
dc.contributor.author | Xie, S | - |
dc.contributor.author | Cui, L | - |
dc.contributor.author | Wang, X | - |
dc.date.accessioned | 2019-09-10T02:42:50Z | - |
dc.date.available | 2019-09-10T02:42:50Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Microchimica Acta, 2018, v. 185 n. 8, article no. 375 | - |
dc.identifier.issn | 0026-3672 | - |
dc.identifier.uri | http://hdl.handle.net/10722/275452 | - |
dc.description.abstract | An innovative signal amplification strategy assisted by RNase H is described here for the detection of DNA targets in a universal fashion. A tailor-made RNA molecular beacon (RMB) conjugated with a fluorescence resonance energy transfer (FRET) pair (fluorophore and quencher) was designed, characterized, and combined with the employment of RNase H. Its performance is compared to that of other nucleases including Exonuclease III and T7 exonuclease. Fluorometry, performed best at excitation/emission wavelengths of 490/520 nm, gives an amazingly low detection limit of 23 fM for target DNA. The method was verified by the determination of human hemochromatosis (HFE) gene. It is perceived that the method represents a versatile tool for the detection of a wide range of targets. | - |
dc.language | eng | - |
dc.publisher | Springer-Verlag Wien. The Journal's web site is located at http://www.springer.at/mikro | - |
dc.relation.ispartof | Microchimica Acta | - |
dc.subject | DNA | - |
dc.subject | Detection limit | - |
dc.subject | Exonuclease III | - |
dc.subject | Sensitivity | - |
dc.subject | Selectivity | - |
dc.subject | T7 exonuclease | - |
dc.subject | Signal amplification | - |
dc.title | RNase H Meets Molecular Beacons: An Ultrasensitive Fluorometric Assay for Nucleic Acids | - |
dc.type | Article | - |
dc.identifier.email | Chen, J: abchen@hkucc.hku.hk | - |
dc.identifier.authority | Chen, J=rp01316 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1007/s00604-018-2909-0 | - |
dc.identifier.pmid | 30008087 | - |
dc.identifier.scopus | eid_2-s2.0-85050072058 | - |
dc.identifier.hkuros | 304896 | - |
dc.identifier.hkuros | 305029 | - |
dc.identifier.volume | 185 | - |
dc.identifier.issue | 8 | - |
dc.identifier.spage | article no. 375 | - |
dc.identifier.epage | article no. 375 | - |
dc.identifier.isi | WOS:000438876300001 | - |
dc.publisher.place | Austria | - |
dc.identifier.issnl | 0026-3672 | - |